Q820I7 (PROA_NITEU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	NE2158

Organism

Nitrosomonas europaea (strain ATCC 19718 / NBRC 14298) [Complete proteome] [HAMAP]

Taxonomic identifier

228410 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Nitrosomonadaceae › Nitrosomonas › Nitrosomonas europaea

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity. HAMAP-Rule MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP-Rule MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP-Rule MF_00412
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Gamma-glutamyl phosphate reductase HAMAP-Rule MF_00412		PRO_0000189757

Sequences

	Sequence		Length	Mass (Da)
	Q820I7 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 96D624B0DAC10931	FASTA	422	45,013
	10 20 30 40 50 60 MEKTEKIQAD MQALGRAARA AARIVAKADT AVKNHALIAM ARAIRCHEAS LLAANAADVA 70 80 90 100 110 120 QARNKGLEPA MIDRLTLTPK GIASMAAGLE QIAALSDPIG AVTDLDYRPS GIQVGRMRVP 130 140 150 160 170 180 LGVIAIIYEA RPNVTADAAG LCLKAGNAAI LRGGSEAIQS NQAIAACVQE GLRSAGLPEH 190 200 210 220 230 240 AVQVVETTDR AAVGELITMS EYVDMVVPRG GKGLIERIAN EARVPVIKHL DGVCHVYVDL 250 260 270 280 290 300 SADLEKAVRV ADNAKTQRYG TCNTMETLLV HAGIAERFLP RICKILLEKG VELRGDEAAR 310 320 330 340 350 360 ALVAGIKPAV EEDWYAEYLA PVLSVRIVED IDQAITHIAT YGSQHTDAIV TEDYSRARQF 370 380 390 400 410 420 LREVDSSSVM INASTRFADG FEYGLGAEIG ISTDKLHARG PVGLEGLTSQ KFIVLGDGHI RE

References

[1]

"Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea." Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J. J. Bacteriol. 185:2759-2773(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19718 / NBRC 14298.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL954747 Genomic DNA. Translation: CAD86069.1.
RefSeq	NP_842162.1. NC_004757.1.
3D structure databases
ProteinModelPortal	Q820I7.
ModBase	Search...
Protein-protein interaction databases
STRING	228410.NE2158.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD86069; CAD86069; NE2158.
GeneID	1083120.
KEGG	neu:NE2158.
PATRIC	22715692. VBINitEur56163_2427.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
HOGENOM	HOG000246356.
KO	K00147.
OMA	ISMPEYV.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	NEUR228410:GJNO-2199-MONOMER.
UniPathway	UPA00098; UER00360.
Family and domain databases
Gene3D	3.40.309.10. 1 hit. 3.40.605.10. 2 hits.
HAMAP	MF_00412. ProA.
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
PANTHER	PTHR11063:SF1. PTHR11063:SF1. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_NITEU

Accession

Primary (citable) accession number: Q820I7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program