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Q820I4

- Q820I4_NITEU

UniProt

Q820I4 - Q820I4_NITEU

Protein

Endonuclease III

Gene

nth

Organism
Nitrosomonas europaea (strain ATCC 19718 / NBRC 14298)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity.UniRule annotation
    DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

    Catalytic activityi

    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi187 – 1871Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi194 – 1941Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi197 – 1971Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi203 – 2031Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
    3. DNA binding Source: UniProtKB-HAMAP
    4. DNA N-glycosylase activity Source: UniProtKB-HAMAP
    5. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. base-excision repair Source: InterPro

    Keywords - Molecular functioni

    GlycosidaseUniRule annotation, Hydrolase, LyaseUniRule annotationImported

    Keywords - Biological processi

    DNA damage, DNA repairUniRule annotation

    Keywords - Ligandi

    4Fe-4SUniRule annotation, DNA-bindingUniRule annotation, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciNEUR228410:GJNO-2267-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endonuclease IIIUniRule annotation (EC:4.2.99.18UniRule annotation)
    Alternative name(s):
    DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
    Gene namesi
    Name:nthUniRule annotationImported
    Ordered Locus Names:NE2223Imported
    OrganismiNitrosomonas europaea (strain ATCC 19718 / NBRC 14298)Imported
    Taxonomic identifieri228410 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas
    ProteomesiUP000001416: Chromosome

    Interactioni

    Protein-protein interaction databases

    STRINGi228410.NE2223.

    Structurei

    3D structure databases

    ProteinModelPortaliQ820I4.
    SMRiQ820I4. Positions 1-208.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini100 – 12728HhHUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Nth/MutY family.UniRule annotation
    Contains 1 HhH domain.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000252206.
    KOiK10773.
    OMAiVDAHHWL.
    OrthoDBiEOG6H4KC5.
    PhylomeDBiQ820I4.

    Family and domain databases

    Gene3Di1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    HAMAPiMF_00942. Nth.
    InterProiIPR011257. DNA_glycosylase.
    IPR004036. Endonuclease-III-like_CS2.
    IPR004035. Endouclease-III_FeS-bd_BS.
    IPR003651. Endouclease3_FeS-loop_motif.
    IPR003265. HhH-GPD_domain.
    IPR000445. HhH_motif.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR023170. HTH_base_excis_C.
    IPR005759. Nth.
    [Graphical view]
    PfamiPF10576. EndIII_4Fe-2S. 1 hit.
    PF00633. HHH. 1 hit.
    PF00730. HhH-GPD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001435. Nth. 1 hit.
    SMARTiSM00478. ENDO3c. 1 hit.
    SM00525. FES. 1 hit.
    SM00278. HhH1. 1 hit.
    [Graphical view]
    SUPFAMiSSF48150. SSF48150. 1 hit.
    TIGRFAMsiTIGR01083. nth. 1 hit.
    PROSITEiPS00764. ENDONUCLEASE_III_1. 1 hit.
    PS01155. ENDONUCLEASE_III_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q820I4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNTTKRREIF TRFRAANPRP TTELEYQTPF QLLIAVILSA QATDKSVNLA    50
    TRKLFLVADT PEKILQLGET GLSPFIQRIG LFRTKTRNIL ATCQLLIEQY 100
    NGEVPRTRTE LEKLPGVGRK TASVILNTAF GEPTIAVDTH IFRVANRIGI 150
    APGKNVLEVE RKLLKVVPDE FRHDAHHWLI LHGRYICKAR KPLCHQCLIV 200
    DLCEFKEKNL EGTASSLDMK QLT 223
    Length:223
    Mass (Da):25,299
    Last modified:June 1, 2003 - v1
    Checksum:iE99B10A3009DFD50
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL954747 Genomic DNA. Translation: CAD86135.1.
    RefSeqiNP_842225.1. NC_004757.1.

    Genome annotation databases

    EnsemblBacteriaiCAD86135; CAD86135; NE2223.
    GeneIDi1083188.
    KEGGineu:NE2223.
    PATRICi22715833. VBINitEur56163_2495.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL954747 Genomic DNA. Translation: CAD86135.1 .
    RefSeqi NP_842225.1. NC_004757.1.

    3D structure databases

    ProteinModelPortali Q820I4.
    SMRi Q820I4. Positions 1-208.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 228410.NE2223.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD86135 ; CAD86135 ; NE2223 .
    GeneIDi 1083188.
    KEGGi neu:NE2223.
    PATRICi 22715833. VBINitEur56163_2495.

    Phylogenomic databases

    HOGENOMi HOG000252206.
    KOi K10773.
    OMAi VDAHHWL.
    OrthoDBi EOG6H4KC5.
    PhylomeDBi Q820I4.

    Enzyme and pathway databases

    BioCyci NEUR228410:GJNO-2267-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    HAMAPi MF_00942. Nth.
    InterProi IPR011257. DNA_glycosylase.
    IPR004036. Endonuclease-III-like_CS2.
    IPR004035. Endouclease-III_FeS-bd_BS.
    IPR003651. Endouclease3_FeS-loop_motif.
    IPR003265. HhH-GPD_domain.
    IPR000445. HhH_motif.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR023170. HTH_base_excis_C.
    IPR005759. Nth.
    [Graphical view ]
    Pfami PF10576. EndIII_4Fe-2S. 1 hit.
    PF00633. HHH. 1 hit.
    PF00730. HhH-GPD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001435. Nth. 1 hit.
    SMARTi SM00478. ENDO3c. 1 hit.
    SM00525. FES. 1 hit.
    SM00278. HhH1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48150. SSF48150. 1 hit.
    TIGRFAMsi TIGR01083. nth. 1 hit.
    PROSITEi PS00764. ENDONUCLEASE_III_1. 1 hit.
    PS01155. ENDONUCLEASE_III_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea."
      Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.
      J. Bacteriol. 185:2759-2773(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 19718 / NBRC 14298Imported.

    Entry informationi

    Entry nameiQ820I4_NITEU
    AccessioniPrimary (citable) accession number: Q820I4
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 1, 2003
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3