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Q820I4

- Q820I4_NITEU

UniProt

Q820I4 - Q820I4_NITEU

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Protein

Endonuclease III

Gene

nth

Organism
Nitrosomonas europaea (strain ATCC 19718 / NBRC 14298)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity.UniRule annotation
DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi187 – 1871Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi194 – 1941Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi197 – 1971Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi203 – 2031Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
  3. DNA binding Source: UniProtKB-HAMAP
  4. DNA N-glycosylase activity Source: UniProtKB-HAMAP
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. base-excision repair Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotation, Hydrolase, LyaseUniRule annotationImported

Keywords - Biological processi

DNA damage, DNA repairUniRule annotation

Keywords - Ligandi

4Fe-4SUniRule annotation, DNA-bindingUniRule annotation, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciNEUR228410:GJNO-2267-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease IIIUniRule annotation (EC:4.2.99.18UniRule annotation)
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
Gene namesi
Name:nthUniRule annotationImported
Ordered Locus Names:NE2223Imported
OrganismiNitrosomonas europaea (strain ATCC 19718 / NBRC 14298)Imported
Taxonomic identifieri228410 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas
ProteomesiUP000001416: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi228410.NE2223.

Structurei

3D structure databases

ProteinModelPortaliQ820I4.
SMRiQ820I4. Positions 1-208.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini100 – 12728HhHUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the Nth/MutY family.UniRule annotation
Contains 1 HhH domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252206.
KOiK10773.
OMAiVDAHHWL.
OrthoDBiEOG6H4KC5.
PhylomeDBiQ820I4.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPiMF_00942. Nth.
InterProiIPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR004035. Endouclease-III_FeS-bd_BS.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view]
PfamiPF10576. EndIII_4Fe-2S. 1 hit.
PF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
PIRSFiPIRSF001435. Nth. 1 hit.
SMARTiSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
TIGRFAMsiTIGR01083. nth. 1 hit.
PROSITEiPS00764. ENDONUCLEASE_III_1. 1 hit.
PS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q820I4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNTTKRREIF TRFRAANPRP TTELEYQTPF QLLIAVILSA QATDKSVNLA
60 70 80 90 100
TRKLFLVADT PEKILQLGET GLSPFIQRIG LFRTKTRNIL ATCQLLIEQY
110 120 130 140 150
NGEVPRTRTE LEKLPGVGRK TASVILNTAF GEPTIAVDTH IFRVANRIGI
160 170 180 190 200
APGKNVLEVE RKLLKVVPDE FRHDAHHWLI LHGRYICKAR KPLCHQCLIV
210 220
DLCEFKEKNL EGTASSLDMK QLT
Length:223
Mass (Da):25,299
Last modified:June 1, 2003 - v1
Checksum:iE99B10A3009DFD50
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL954747 Genomic DNA. Translation: CAD86135.1.
RefSeqiNP_842225.1. NC_004757.1.

Genome annotation databases

EnsemblBacteriaiCAD86135; CAD86135; NE2223.
GeneIDi1083188.
KEGGineu:NE2223.
PATRICi22715833. VBINitEur56163_2495.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL954747 Genomic DNA. Translation: CAD86135.1 .
RefSeqi NP_842225.1. NC_004757.1.

3D structure databases

ProteinModelPortali Q820I4.
SMRi Q820I4. Positions 1-208.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 228410.NE2223.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD86135 ; CAD86135 ; NE2223 .
GeneIDi 1083188.
KEGGi neu:NE2223.
PATRICi 22715833. VBINitEur56163_2495.

Phylogenomic databases

HOGENOMi HOG000252206.
KOi K10773.
OMAi VDAHHWL.
OrthoDBi EOG6H4KC5.
PhylomeDBi Q820I4.

Enzyme and pathway databases

BioCyci NEUR228410:GJNO-2267-MONOMER.

Family and domain databases

Gene3Di 1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPi MF_00942. Nth.
InterProi IPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR004035. Endouclease-III_FeS-bd_BS.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view ]
Pfami PF10576. EndIII_4Fe-2S. 1 hit.
PF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001435. Nth. 1 hit.
SMARTi SM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view ]
SUPFAMi SSF48150. SSF48150. 1 hit.
TIGRFAMsi TIGR01083. nth. 1 hit.
PROSITEi PS00764. ENDONUCLEASE_III_1. 1 hit.
PS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea."
    Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.
    J. Bacteriol. 185:2759-2773(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 19718 / NBRC 14298Imported.

Entry informationi

Entry nameiQ820I4_NITEU
AccessioniPrimary (citable) accession number: Q820I4
Entry historyi
Integrated into UniProtKB/TrEMBL: June 1, 2003
Last sequence update: June 1, 2003
Last modified: October 1, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3