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Q820I4 (Q820I4_NITEU) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Endonuclease III PIRNR PIRNR001435 HAMAP-Rule MF_00942

EC=4.2.99.18 PIRNR PIRNR001435 HAMAP-Rule MF_00942
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase HAMAP-Rule MF_00942
Gene names
Name:nth HAMAP-Rule MF_00942 EMBL CAD86135.1
Ordered Locus Names:NE2223 EMBL CAD86135.1
OrganismNitrosomonas europaea (strain ATCC 19718 / NBRC 14298) [Complete proteome] [HAMAP] EMBL CAD86135.1
Taxonomic identifier228410 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas

Protein attributes

Sequence length223 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity By similarity. PIRNR PIRNR001435

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate By similarity. HAMAP-Rule MF_00942

Catalytic activity

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. PIRNR PIRNR001435 HAMAP-Rule MF_00942

Cofactor

Binds 1 4Fe-4S cluster By similarity. PIRNR PIRNR001435 HAMAP-Rule MF_00942

Sequence similarities

Belongs to the Nth/MutY family. PIRNR PIRNR001435 HAMAP-Rule MF_00942

Contains 1 HhH domain. HAMAP-Rule MF_00942

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain100 – 12728HhH By similarity HAMAP-Rule MF_00942

Sites

Metal binding1871Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_00942
Metal binding1941Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_00942
Metal binding1971Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_00942
Metal binding2031Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_00942

Sequences

Sequence LengthMass (Da)Tools
Q820I4 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: E99B10A3009DFD50

FASTA22325,299
        10         20         30         40         50         60 
MNTTKRREIF TRFRAANPRP TTELEYQTPF QLLIAVILSA QATDKSVNLA TRKLFLVADT 

        70         80         90        100        110        120 
PEKILQLGET GLSPFIQRIG LFRTKTRNIL ATCQLLIEQY NGEVPRTRTE LEKLPGVGRK 

       130        140        150        160        170        180 
TASVILNTAF GEPTIAVDTH IFRVANRIGI APGKNVLEVE RKLLKVVPDE FRHDAHHWLI 

       190        200        210        220 
LHGRYICKAR KPLCHQCLIV DLCEFKEKNL EGTASSLDMK QLT 

« Hide

References

[1]"Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea."
Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.
J. Bacteriol. 185:2759-2773(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19718 / NBRC 14298.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL954747 Genomic DNA. Translation: CAD86135.1.
RefSeqNP_842225.1. NC_004757.1.

3D structure databases

ProteinModelPortalQ820I4.
SMRQ820I4. Positions 1-208.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING228410.NE2223.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD86135; CAD86135; NE2223.
GeneID1083188.
KEGGneu:NE2223.
PATRIC22715833. VBINitEur56163_2495.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000252206.
KOK10773.
OMAVDAHHWL.
OrthoDBEOG6H4KC5.
PhylomeDBQ820I4.

Enzyme and pathway databases

BioCycNEUR228410:GJNO-2267-MONOMER.

Family and domain databases

Gene3D1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPMF_00942. Nth.
InterProIPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR004035. Endouclease-III_FeS-bd_BS.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view]
PfamPF10576. EndIII_4Fe-2S. 1 hit.
PF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
PIRSFPIRSF001435. Nth. 1 hit.
SMARTSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMSSF48150. SSF48150. 1 hit.
TIGRFAMsTIGR01083. nth. 1 hit.
PROSITEPS00764. ENDONUCLEASE_III_1. 1 hit.
PS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ820I4_NITEU
AccessionPrimary (citable) accession number: Q820I4
Entry history
Integrated into UniProtKB/TrEMBL: June 1, 2003
Last sequence update: June 1, 2003
Last modified: June 11, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)