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Protein

Endonuclease III

Gene

nth

Organism
Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi187Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi194Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi197Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi203Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotation, GlycosidaseUniRule annotation, Hydrolase, LyaseUniRule annotationImported
Biological processDNA damage, DNA repairUniRule annotation
Ligand4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciNEUR228410:G1G1I-2353-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease IIIUniRule annotation (EC:4.2.99.18UniRule annotation)
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
Gene namesi
Name:nthUniRule annotationImported
Ordered Locus Names:NE2223Imported
OrganismiNitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)Imported
Taxonomic identifieri228410 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas
Proteomesi

Interactioni

Protein-protein interaction databases

STRINGi228410.NE2223

Structurei

3D structure databases

ProteinModelPortaliQ820I4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 185ENDO3cInterPro annotationAdd BLAST148

Sequence similaritiesi

Belongs to the Nth/MutY family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CSM Bacteria
COG0177 LUCA
HOGENOMiHOG000252206
KOiK10773
OMAiKAKNPLC
OrthoDBiPOG091H021U
PhylomeDBiQ820I4

Family and domain databases

CDDicd00056 ENDO3c, 1 hit
Gene3Di1.10.1670.10, 2 hits
HAMAPiMF_00942 Nth, 1 hit
InterProiView protein in InterPro
IPR011257 DNA_glycosylase
IPR004036 Endonuclease-III-like_CS2
IPR003651 Endonuclease3_FeS-loop_motif
IPR004035 Endouclease-III_FeS-bd_BS
IPR003265 HhH-GPD_domain
IPR000445 HhH_motif
IPR023170 HTH_base_excis_C
IPR005759 Nth
PfamiView protein in Pfam
PF10576 EndIII_4Fe-2S, 1 hit
PF00633 HHH, 1 hit
PF00730 HhH-GPD, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SM00525 FES, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
TIGRFAMsiTIGR01083 nth, 1 hit
PROSITEiView protein in PROSITE
PS00764 ENDONUCLEASE_III_1, 1 hit
PS01155 ENDONUCLEASE_III_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q820I4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTTKRREIF TRFRAANPRP TTELEYQTPF QLLIAVILSA QATDKSVNLA
60 70 80 90 100
TRKLFLVADT PEKILQLGET GLSPFIQRIG LFRTKTRNIL ATCQLLIEQY
110 120 130 140 150
NGEVPRTRTE LEKLPGVGRK TASVILNTAF GEPTIAVDTH IFRVANRIGI
160 170 180 190 200
APGKNVLEVE RKLLKVVPDE FRHDAHHWLI LHGRYICKAR KPLCHQCLIV
210 220
DLCEFKEKNL EGTASSLDMK QLT
Length:223
Mass (Da):25,299
Last modified:June 1, 2003 - v1
Checksum:iE99B10A3009DFD50
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL954747 Genomic DNA Translation: CAD86135.1
RefSeqiWP_011112716.1, NC_004757.1

Genome annotation databases

EnsemblBacteriaiCAD86135; CAD86135; NE2223
KEGGineu:NE2223

Similar proteinsi

Entry informationi

Entry nameiQ820I4_NITEU
AccessioniPrimary (citable) accession number: Q820I4
Entry historyiIntegrated into UniProtKB/TrEMBL: June 1, 2003
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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