ID   NSP3_ROTHW              Reviewed;         313 AA.
AC   Q82054; Q8B6X7; Q8B6X8;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   27-MAR-2024, entry version 76.
DE   RecName: Full=Non-structural protein 3 {ECO:0000255|HAMAP-Rule:MF_04094};
DE            Short=NSP3 {ECO:0000255|HAMAP-Rule:MF_04094};
DE   AltName: Full=NCVP4 {ECO:0000255|HAMAP-Rule:MF_04094};
DE   AltName: Full=Non-structural RNA-binding protein 34 {ECO:0000255|HAMAP-Rule:MF_04094};
DE            Short=NS34 {ECO:0000255|HAMAP-Rule:MF_04094};
OS   Rotavirus A (strain RVA/Human/United States/Wa/1974/G1P1A[8]) (RV-A).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Sedoreoviridae; Rotavirus; Rotavirus A.
OX   NCBI_TaxID=10962;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=7871749; DOI=10.1006/viro.1995.1087;
RA   Rao C.D., Das M., Ilango P., Lalwani R., Rao B.S., Gowda K.;
RT   "Comparative nucleotide and amino acid sequence analysis of the sequence-
RT   specific RNA-binding rotavirus nonstructural protein NSP3.";
RL   Virology 207:327-333(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 4-61 AND 279-313.
RX   PubMed=1339264; DOI=10.1007/bf01309651;
RA   Mendez E., Arias C.F., Lopez S.;
RT   "Genomic rearrangements in human rotavirus strain Wa; analysis of
RT   rearranged RNA segment 7.";
RL   Arch. Virol. 125:331-338(1992).
CC   -!- FUNCTION: Plays an important role in stimulating the translation of
CC       viral mRNAs. These mRNAs are capped but not polyadenylated, instead
CC       terminating in a conserved sequence 'GACC' at the 3' that is recognized
CC       by NSP3, which competes with host PABPC1 for EIF4G1 binding. The
CC       interaction between NSP3 and host EIF4G1 stabilizes the EIF4E-EIF4G1
CC       interaction, thereby facilitating the initiation of capped mRNA
CC       translation. {ECO:0000255|HAMAP-Rule:MF_04094}.
CC   -!- SUBUNIT: Homodimer. Interacts (via the coiled-coil region) with host
CC       ZC3H7B (via LD motif). Interacts with host EIF4G1. {ECO:0000255|HAMAP-
CC       Rule:MF_04094}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000255|HAMAP-Rule:MF_04094}.
CC   -!- SIMILARITY: Belongs to the rotavirus NSP3 family. {ECO:0000255|HAMAP-
CC       Rule:MF_04094}.
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DR   EMBL; X81434; CAA57193.1; -; mRNA.
DR   EMBL; AH003952; AAN15754.1; -; Genomic_RNA.
DR   EMBL; AH003952; AAN15755.1; -; Genomic_RNA.
DR   PIR; S51731; S51731.
DR   SMR; Q82054; -.
DR   Proteomes; UP000006581; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-UniRule.
DR   CDD; cd20714; NSP3_rotavirus; 1.
DR   Gene3D; 3.30.70.1610; -; 1.
DR   Gene3D; 1.20.5.970; Nonstructural RNA-binding protein; 1.
DR   Gene3D; 6.10.280.20; Rotavirus non-structural protein NSP3, N-terminal domain; 1.
DR   HAMAP; MF_04094; ROTA_A_NSP3; 1.
DR   HAMAP; MF_04090; ROTA_NSP3; 1.
DR   InterPro; IPR042519; NSP3_N_rotavirus.
DR   InterPro; IPR036082; NSP3_sf.
DR   InterPro; IPR002873; Rotavirus_NSP3.
DR   Pfam; PF01665; Rota_NSP3; 1.
DR   SUPFAM; SSF69903; NSP3 homodimer; 1.
DR   SUPFAM; SSF58030; Rotavirus nonstructural proteins; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; Host cytoplasm; Host-virus interaction; RNA-binding;
KW   Translation regulation.
FT   CHAIN           1..313
FT                   /note="Non-structural protein 3"
FT                   /id="PRO_0000369458"
FT   REGION          1..149
FT                   /note="RNA-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04094"
FT   REGION          150..206
FT                   /note="Dimerization"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04094"
FT   REGION          170..234
FT                   /note="Interaction with host ZC3H7B"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04094"
FT   REGION          208..313
FT                   /note="Interaction with host EIF4G1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04094"
FT   COILED          166..237
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04094"
FT   CONFLICT        30..35
FT                   /note="LELMGI -> FEFMGF (in Ref. 2; AAN15755)"
FT   CONFLICT        58
FT                   /note="G -> C (in Ref. 2; AAN15755)"
FT   CONFLICT        284
FT                   /note="L -> F (in Ref. 2; AAN15754)"
SQ   SEQUENCE   313 AA;  36023 MW;  658BA1623464F66C CRC64;
     MLKMESTQQM VSSIINTSFE AAVVAATSTL ELMGIQYDYN EVFTRVKSKF DYVMDDSGVK
     NNLLGKAITI AQALNGKFGS AIRNRNWMTD SKTVAKLDED VSKLRMTLSS KGIDQKMRVL
     NACFSVKRIP GKSSSIIKCT RLMKDKIEGG EVEVDDSYVD EKMEIDTIDW KSRYDQLEKR
     FEALKQRVNE KYNTWVQKAK KVNENMYSLQ NVISQQQNQI SDLQQYCNKL EADLQGKFSS
     LVSSVEWYLR SMELPDDVKN DIEQQLNSID AINPINAIDD IESLIRNLIQ DYDRTFLMLK
     GLLKQCNYEY AYE
//