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Q82053

- NSP3_ROTHT

UniProt

Q82053 - NSP3_ROTHT

Protein

Non-structural protein 3

Gene
N/A
Organism
Rotavirus A (strain Human/United Kingdom/ST3/1975 G4-P2A[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) (Rotavirus A (strain St. Thomas 3))
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Involved in translation of viral mRNAs and shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection By similarity.By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. regulation of translation Source: UniProtKB-KW
    2. suppression by virus of host gene expression Source: UniProtKB-KW

    Keywords - Biological processi

    Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Translation regulation

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-structural protein 3
    Short name:
    NSP3
    Alternative name(s):
    NCVP4
    Non-structural RNA-binding protein 34
    Short name:
    NS34
    OrganismiRotavirus A (strain Human/United Kingdom/ST3/1975 G4-P2A[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) (Rotavirus A (strain St. Thomas 3))
    Taxonomic identifieri10960 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000007048: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 313313Non-structural protein 3PRO_0000369453Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ82053.
    SMRiQ82053. Positions 7-163, 206-312.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni4 – 149146RNA-bindingBy similarityAdd
    BLAST
    Regioni150 – 20657DimerizationBy similarityAdd
    BLAST
    Regioni170 – 23465Interaction with ZC3H7BBy similarityAdd
    BLAST
    Regioni208 – 313106Interaction with EIF4G1By similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili166 – 23772Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the rotavirus A NSP3 family.Curated

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    InterProiIPR002873. Rotavirus_NSP3.
    [Graphical view]
    PfamiPF01665. Rota_NSP3. 1 hit.
    [Graphical view]
    SUPFAMiSSF69903. SSF69903. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q82053-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKMESTQQM VSSIINTSFE AAVVAATSTL ELMGIQYDYN EVFTRVKSKF    50
    DYVMDDSGVK NNLLGKAITI AQALNGKFGS AIRNRNWMSD SKTVAKLDED 100
    VNKLRMTLSS KGIDQKMRVL NACFSVKRIP GKSSSIIKCT RLMKDKIERG 150
    EVEVDDSYVD EKMEIDTIDW KSRYDQLEKR FESLKQRVNE KYNTWVQKAK 200
    KVNENMYSLQ NVISQQQNQI ADLQQYCNKL EADLQGKFSS LVSSVEWYLR 250
    SMELPDDVKT DIEQQLNSID LINPINAIDD IESLIRNLIQ DYDRTFLMLK 300
    GLLKQCNYEY AYE 313
    Length:313
    Mass (Da):36,164
    Last modified:November 1, 1996 - v1
    Checksum:i75F9C921A304765E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti71 – 711A → D in ABV53294. (PubMed:18786998)Curated
    Sequence conflicti89 – 891S → T in ABV53294. (PubMed:18786998)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X81436 mRNA. Translation: CAA57195.1.
    EF672614 Genomic RNA. Translation: ABV53294.1.
    PIRiS51730.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X81436 mRNA. Translation: CAA57195.1 .
    EF672614 Genomic RNA. Translation: ABV53294.1 .
    PIRi S51730.

    3D structure databases

    ProteinModelPortali Q82053.
    SMRi Q82053. Positions 7-163, 206-312.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR002873. Rotavirus_NSP3.
    [Graphical view ]
    Pfami PF01665. Rota_NSP3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69903. SSF69903. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Comparative nucleotide and amino acid sequence analysis of the sequence-specific RNA-binding rotavirus nonstructural protein NSP3."
      Rao C.D., Das M., Ilango P., Lalwani R., Rao B.S., Gowda K.
      Virology 207:327-333(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions."
      Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T., Patton J.T.
      J. Virol. 82:11106-11116(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 4-313.

    Entry informationi

    Entry nameiNSP3_ROTHT
    AccessioniPrimary (citable) accession number: Q82053
    Secondary accession number(s): B3SRW9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3