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Q82051

- NSP3_ROTH6

UniProt

Q82051 - NSP3_ROTH6

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Protein
Non-structural protein 3
Gene
N/A
Organism
Rotavirus A (strain Human/Indonesia/69M/1980 G8-P4[10]-I2-R2-C2-M2-A2-N2-T2-E2-H2) (RV-A)
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in translation of viral mRNAs and shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection By similarity.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 3
Short name:
NSP3
Alternative name(s):
NCVP4
Non-structural RNA-binding protein 34
Short name:
NS34
OrganismiRotavirus A (strain Human/Indonesia/69M/1980 G8-P4[10]-I2-R2-C2-M2-A2-N2-T2-E2-H2) (RV-A)
Taxonomic identifieri10947 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000001455: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313Non-structural protein 3
PRO_0000369448Add
BLAST

Interactioni

Subunit structurei

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ82051.
SMRiQ82051. Positions 7-163, 206-312.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 149146RNA-binding By similarity
Add
BLAST
Regioni150 – 20657Dimerization By similarity
Add
BLAST
Regioni170 – 23465Interaction with ZC3H7B By similarity
Add
BLAST
Regioni208 – 313106Interaction with EIF4G1 By similarity
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili166 – 23772 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the rotavirus A NSP3 family.

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamiPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMiSSF69903. SSF69903. 1 hit.

Sequencei

Sequence statusi: Complete.

Q82051-1 [UniParc]FASTAAdd to Basket

« Hide

MLKMESTQQM VSSIINSSFE AAVVAATSTL ELMGIQYDYN EVYTRVKSKF    50
DLVMDDSGVK NNLMGKAATI DQALNGKFSS SIRNRNWMTD SKTDARLDED 100
VNKLRLLLSS KGIDQKMRVL NACFNVKRIP GKSSSVIRCT RLMKEKIERG 150
EVEVDDAFVE EKMEVDTIDW KSRYEQLEKR FESLKQRVNE KYNNWVIKAR 200
KDNENMYSLQ NVISQQQAHI NELQIYNNKL ERDLQSKIGS VISSIEWYLR 250
SMELSDDIKS DIEQQLNSID QINPVNAYDD FESILRNLIS DYDRMFIMFK 300
GLLQQSNYIY TYE 313
Length:313
Mass (Da):36,493
Last modified:November 1, 1996 - v1
Checksum:i98022A6F7EE5514D
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 451R → K in ABV53230. 1 Publication
Sequence conflicti94 – 941D → V in ABV53230. 1 Publication
Sequence conflicti202 – 2021D → V in ABV53230. 1 Publication
Sequence conflicti207 – 2071Y → N in ABV53230. 1 Publication
Sequence conflicti278 – 2781Y → F in ABV53230. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X81425 mRNA. Translation: CAA57184.1.
EF672558 Genomic DNA. Translation: ABV53230.1.
PIRiS51728.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X81425 mRNA. Translation: CAA57184.1 .
EF672558 Genomic DNA. Translation: ABV53230.1 .
PIRi S51728.

3D structure databases

ProteinModelPortali Q82051.
SMRi Q82051. Positions 7-163, 206-312.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002873. Rotavirus_NSP3.
[Graphical view ]
Pfami PF01665. Rota_NSP3. 1 hit.
[Graphical view ]
SUPFAMi SSF69903. SSF69903. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative nucleotide and amino acid sequence analysis of the sequence-specific RNA-binding rotavirus nonstructural protein NSP3."
    Rao C.D., Das M., Ilango P., Lalwani R., Rao B.S., Gowda K.
    Virology 207:327-333(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions."
    Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T., Patton J.T.
    J. Virol. 82:11106-11116(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 3-313.

Entry informationi

Entry nameiNSP3_ROTH6
AccessioniPrimary (citable) accession number: Q82051
Secondary accession number(s): B3SRQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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