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Q82050 (NSP3_ROTHK) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-structural protein 3

Short name=NSP3
Alternative name(s):
NCVP4
Non-structural RNA-binding protein 34
Short name=NS34
OrganismRotavirus A (strain Human/Japan/KU/1995 G1-P1A[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) [Complete proteome]
Taxonomic identifier10952 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in translation of viral mRNAs and shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection By similarity.

Subunit structure

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 By similarity.

Sequence similarities

Belongs to the rotavirus A NSP3 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313Non-structural protein 3
PRO_0000369450

Regions

Region4 – 149146RNA-binding By similarity
Region150 – 20657Dimerization By similarity
Region170 – 23465Interaction with ZC3H7B By similarity
Region208 – 313106Interaction with EIF4G1 By similarity
Coiled coil166 – 23772 Potential

Experimental info

Sequence conflict1 – 33Missing in BAA84968. Ref.2
Sequence conflict71 – 766DQALNG → GPACNE in BAA84968. Ref.2
Sequence conflict2761N → G in BAA84968. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q82050 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 71185ADCE4276F58

FASTA31336,218
        10         20         30         40         50         60 
MLKMESTQQM VSSIINTSFE AAVVAATSTL ELMGIQYDYN EVFTRVKSKF DYVMDDSGVK 

        70         80         90        100        110        120 
NNLLGKAITI DQALNGKFGS AIRNRNWMID SKTVAKLDED VNKLRMTLSS KGIDQKMRVL 

       130        140        150        160        170        180 
NACFSVKRIP GKSSSIIKCT RLMKDKLERG EVEVDDSYVD EKMEIDTIDW KSRYDQLEKR 

       190        200        210        220        230        240 
FESLKQRVNE KYNAWVQKAK KVNENMYSLQ NVISQQQNQI ADLQQYCNKL EVDLQGKFSS 

       250        260        270        280        290        300 
LVSSVEWYLR SMELPDDVKT DVEQQLNSID LINPINAIDD IESLIRNLIQ DYDRTFLMLK 

       310 
GLLKQCNYEY AYE 

« Hide

References

[1]"Comparative nucleotide and amino acid sequence analysis of the sequence-specific RNA-binding rotavirus nonstructural protein NSP3."
Rao C.D., Das M., Ilango P., Lalwani R., Rao B.S., Gowda K.
Virology 207:327-333(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Taniguchi K.
Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X81435 mRNA. Translation: CAA57194.1.
AB022771 mRNA. Translation: BAA84968.1.
PIRS51727.

3D structure databases

ProteinModelPortalQ82050.
SMRQ82050. Positions 7-163, 206-312.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMSSF69903. SSF69903. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNSP3_ROTHK
AccessionPrimary (citable) accession number: Q82050
Secondary accession number(s): Q9QNA7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families