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Q82049

- NSP3_ROTWI

UniProt

Q82049 - NSP3_ROTWI

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Protein
Non-structural protein 3
Gene
N/A
Organism
Rotavirus A (isolate Human/United States/WI61/1983 G9-P1A[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A)
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in the shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection By similarity.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. suppression by virus of host translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 3
Short name:
NSP3
Alternative name(s):
NCVP4
Non-structural RNA-binding protein 34
Short name:
NS34
OrganismiRotavirus A (isolate Human/United States/WI61/1983 G9-P1A[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A)
Taxonomic identifieri578830 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000006580: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313Non-structural protein 3
PRO_0000369444Add
BLAST

Interactioni

Subunit structurei

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ82049.
SMRiQ82049. Positions 7-163, 206-312.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 149146RNA-binding By similarity
Add
BLAST
Regioni150 – 20657Dimerization By similarity
Add
BLAST
Regioni170 – 23465Interaction with ZC3H7B By similarity
Add
BLAST
Regioni208 – 313106Interaction with EIF4G1 By similarity
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili166 – 23772 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the rotavirus A NSP3 family.

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamiPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMiSSF69903. SSF69903. 1 hit.

Sequencei

Sequence statusi: Complete.

Q82049-1 [UniParc]FASTAAdd to Basket

« Hide

MLKMESTQQM VISVINTSFE AAVVAATSTL ELMGIQYDYN EVFTRVKSKF    50
DYVMDDSGVK NNLLGKAITI DQALNGKLGS AIRNRNWMTD SKTVAKLDED 100
VNKLRMILSS KGIDQKMRVL NACFSVKRIP GKSSSIIKCT RLMKDKIERG 150
EVEVDDSYVD EKMEIDTIDW KFRYDQLEKR FESLKQRVNE KYNTWVQKAK 200
KVNENMYSLQ NVISQQQNQI ADLQQYRNKL ETDLQGKFSS LVSSVEWYLR 250
SMELPDDVKT DIEQQLNSID LINSINAIDD IESLIRNLIQ DYDRTFLMLK 300
GLLKQCNYEY TYE 313
Length:313
Mass (Da):36,376
Last modified:November 1, 1996 - v1
Checksum:i16D63A31BFF3BD18
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121I → S in ABV53302. 1 Publication
Sequence conflicti227 – 2271R → C in ABV53302. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X81437 mRNA. Translation: CAA57196.1.
EF672621 Genomic RNA. Translation: ABV53302.1.
PIRiS51726.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X81437 mRNA. Translation: CAA57196.1 .
EF672621 Genomic RNA. Translation: ABV53302.1 .
PIRi S51726.

3D structure databases

ProteinModelPortali Q82049.
SMRi Q82049. Positions 7-163, 206-312.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002873. Rotavirus_NSP3.
[Graphical view ]
Pfami PF01665. Rota_NSP3. 1 hit.
[Graphical view ]
SUPFAMi SSF69903. SSF69903. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative nucleotide and amino acid sequence analysis of the sequence-specific RNA-binding rotavirus nonstructural protein NSP3."
    Rao C.D., Das M., Ilango P., Lalwani R., Rao B.S., Gowda K.
    Virology 207:327-333(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions."
    Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T., Patton J.T.
    J. Virol. 82:11106-11116(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 3-313.

Entry informationi

Entry nameiNSP3_ROTWI
AccessioniPrimary (citable) accession number: Q82049
Secondary accession number(s): B3SRX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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