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Protein

Non-structural protein 3

Gene
N/A
Organism
Rotavirus A (isolate RVA/Human/United States/WI61/1983/G9P1A[8]) (RV-A)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Plays an important role in stimulating the translation of viral mRNAs. These mRNAs are capped but not polyadenylated, instead terminating in a conserved sequence 'GACC' at the 3' that is recognized by NSP3, which competes with host PABPC1 for EIF4G1 binding. The interaction between NSP3 and host EIF4G1 stabilizes the EIF4E-EIF4G1 interaction, thereby facilitating the initiation of capped mRNA translation.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processHost-virus interaction, Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 3UniRule annotation
Short name:
NSP3UniRule annotation
Alternative name(s):
NCVP4UniRule annotation
Non-structural RNA-binding protein 34UniRule annotation
Short name:
NS34UniRule annotation
OrganismiRotavirus A (isolate RVA/Human/United States/WI61/1983/G9P1A[8]) (RV-A)
Taxonomic identifieri578830 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000006580 Componenti: Genome

Subcellular locationi

  • Host cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003694441 – 313Non-structural protein 3Add BLAST313

Interactioni

Subunit structurei

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ82049
SMRiQ82049
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 149RNA-bindingUniRule annotationAdd BLAST149
Regioni150 – 206DimerizationUniRule annotationAdd BLAST57
Regioni170 – 234Interaction with host ZC3H7BUniRule annotationAdd BLAST65
Regioni208 – 313Interaction with host EIF4G1UniRule annotationAdd BLAST106

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili166 – 237UniRule annotationAdd BLAST72

Sequence similaritiesi

Belongs to the rotavirus NSP3 family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

OrthoDBiVOG090000G7

Family and domain databases

HAMAPiMF_04094 ROTA_A_NSP3, 1 hit
MF_04090 ROTA_NSP3, 1 hit
InterProiView protein in InterPro
IPR036082 NSP3_sf
IPR002873 Rotavirus_NSP3
PfamiView protein in Pfam
PF01665 Rota_NSP3, 1 hit
SUPFAMiSSF69903 SSF69903, 1 hit

Sequencei

Sequence statusi: Complete.

Q82049-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKMESTQQM VISVINTSFE AAVVAATSTL ELMGIQYDYN EVFTRVKSKF
60 70 80 90 100
DYVMDDSGVK NNLLGKAITI DQALNGKLGS AIRNRNWMTD SKTVAKLDED
110 120 130 140 150
VNKLRMILSS KGIDQKMRVL NACFSVKRIP GKSSSIIKCT RLMKDKIERG
160 170 180 190 200
EVEVDDSYVD EKMEIDTIDW KFRYDQLEKR FESLKQRVNE KYNTWVQKAK
210 220 230 240 250
KVNENMYSLQ NVISQQQNQI ADLQQYRNKL ETDLQGKFSS LVSSVEWYLR
260 270 280 290 300
SMELPDDVKT DIEQQLNSID LINSINAIDD IESLIRNLIQ DYDRTFLMLK
310
GLLKQCNYEY TYE
Length:313
Mass (Da):36,376
Last modified:November 1, 1996 - v1
Checksum:i16D63A31BFF3BD18
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12I → S in ABV53302 (PubMed:18786998).1
Sequence conflicti227R → C in ABV53302 (PubMed:18786998).1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81437 mRNA Translation: CAA57196.1
EF672621 Genomic RNA Translation: ABV53302.1
PIRiS51726

Similar proteinsi

Entry informationi

Entry nameiNSP3_ROTWI
AccessioniPrimary (citable) accession number: Q82049
Secondary accession number(s): B3SRX7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health