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Q82045 (NSP1_ROTHT) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-structural protein 1

Short name=NSP1
Alternative name(s):
NCVP2
Non-structural RNA-binding protein 53
Short name=NS53
OrganismRotavirus A (strain Human/United Kingdom/ST3/1975 G4-P2A[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) (Rotavirus A (strain St. Thomas 3)) [Complete proteome]
Taxonomic identifier10960 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length486 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation By similarity.

Subunit structure

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation By similarity.

Subcellular location

Host cytoplasmhost cytoskeleton By similarity.

Domain

The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity By similarity.

Sequence similarities

Belongs to the rotavirus A NSP1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 486486Non-structural protein 1
PRO_0000369086

Regions

Zinc finger42 – 7938 Potential
Region1 – 8181RNA-binding By similarity
Region82 – 17695Important for cytoskeleton localization By similarity
Region313 – 486174Interaction with IRF3 By similarity

Experimental info

Sequence conflict621D → V in ABV53293. Ref.2
Sequence conflict2641E → K in ABV53293. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q82045 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: DFE35AC1AE44252F

FASTA48657,164
        10         20         30         40         50         60 
MATFKDACYY YKRINKLNHV VLKLGVNDTW RPSPPTKYKG WCLDCCQHTD LTYCQGCTMY 

        70         80         90        100        110        120 
HDCQWCSQYG RCFLDSEPHL LRMRTFKNEV TKNDLMNLID MYNTLFPINQ KIVDKFINST 

       130        140        150        160        170        180 
RQHKCRNECM TQWYNHLLMP ITLQSLSIEL DGDVYYVFGY YDSMSDINQT PFSFANLIDI 

       190        200        210        220        230        240 
YDKLLLDNIN FNRMSFLPVA LQQEYALRYF SKSRFISEKR KCVSDLHFSA NVIENLHNPS 

       250        260        270        280        290        300 
FKIQITRNCI ELSSDWNGAC KLVEDVSAYF DMLKTSHIEF YSISTRCRVF TQHKLKMASK 

       310        320        330        340        350        360 
HIKPNYVTSN HRTSATEVHN CKWCSINNSY AVWNDFRVKK IYDNIFNFLR ALVKSNANVG 

       370        380        390        400        410        420 
HCSSQEKIYE HIEDVLDVCD DEKWKTAVTE IFNCLEPVEL DAVKYVLFNH EVNWDVINLL 

       430        440        450        460        470        480 
VQSVGKVPQI LTLNDIVIIM KSIIYEWFDI RYMRNTPMTT FTVDKLRQLC TGVKTVDYDS 


GISDVE 

« Hide

References

[1]"Genetic analysis of NSP1 genes of human rotaviruses isolated from neonates with asymptomatic infection."
Palombo E.A., Bishop R.F.
J. Gen. Virol. 75:3635-3639(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions."
Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T., Patton J.T.
J. Virol. 82:11106-11116(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U11492 mRNA. Translation: AAA75585.1.
EF672613 Genomic RNA. Translation: ABV53293.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamPF00981. Rota_NS53. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNSP1_ROTHT
AccessionPrimary (citable) accession number: Q82045
Secondary accession number(s): B3SRW8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families