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Q82041

- VP3_ROTHC

UniProt

Q82041 - VP3_ROTHC

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Protein
Protein VP3
Gene
N/A
Organism
Rotavirus C (isolate Human/United Kingdom/Bristol/1989) (RV-C)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.

Catalytic activityi

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  4. mRNA guanylyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. viral process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein VP3
Including the following 2 domains:
mRNA guanylyltransferase (EC:2.7.7.50)
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
OrganismiRotavirus C (isolate Human/United Kingdom/Bristol/1989) (RV-C)
Taxonomic identifieri31567 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007664: Genome

Subcellular locationi

Virion Reviewed prediction
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Reviewed prediction.

GO - Cellular componenti

  1. viral nucleocapsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 693693Protein VP3
PRO_0000369872Add
BLAST

Interactioni

Subunit structurei

Interacts with VP1 Reviewed prediction. Interacts with VP2.

Family & Domainsi

Sequence similaritiesi

Belongs to the rotavirus VP3 family.

Family and domain databases

InterProiIPR011181. VP3_Rotav.
[Graphical view]
PfamiPF06929. Rotavirus_VP3. 1 hit.
[Graphical view]
PIRSFiPIRSF004015. LigT_rotavirus. 1 hit.
PROSITEiPS51589. SAM_MT56_VP3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q82041-1 [UniParc]FASTAAdd to Basket

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MRVLGLFERG NNLNFADTYV YTWNQQYSYH ENAFLISNQV ATTIILYLDG    50
ININEVNKAF ELLNSNGIPA LIIKPDHIGI FTSSNFTYDW QYKIVYFHEY 100
TYYKNNEFIV SDEFWLYTNI NELLPYKILY YERGMRELYA GREYTLYNTA 150
TDDDILYKYI YEKDSIMNGT DYKKLYDTNS VKNFVHFMRL LRMRFAVPFD 200
QLSNRITRSR VFSKSRIHIG LRNESIPQAL DNIHSQWINY SANGIVISEL 250
KGLGSYSEKK ISEFGIGQFK NYMNFLTLMF YIKNMKKKPS CTIIGAAPGY 300
WISSMKKYFT IVTYDNKEVD STEHHNRYFT DDDIVNVKTN GVYIDVRSEF 350
KTNDWRQRRK LIEEETIKWL EISYKLLENK RVEAILLKMT AMDGEIPDGY 400
CVHSPTTYRK SEYYLLIDKH IIKRQKIKVT KSLMYNAINT IYSDNVFISG 450
KYSLRGKTEG VLALYCLSNT INQKEKVIQY ANSFSGTCMT VRLNNTYEVD 500
KIIDFKTNSD HTFLPSDFTC SLNTILTSYR GYAGIFGYAI TKDLKSNGNN 550
HIYIIPNARD ENNFDTFGSH LGLSRYSHSK RFSESATTMS GYIFRDMVSG 600
KENMQDTDKD NYASGHVFNA IAHYRFDYTY DIVGWLRLHK TGQFKVKSDI 650
YKEHTDSEIR NAIESAYVYY LLDGDKVGEK YSKKMMEIWE VQV 693
Length:693
Mass (Da):81,316
Last modified:November 1, 1996 - v1
Checksum:iBD9AAF4E3533A175
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X96697 mRNA. Translation: CAA65469.1.
RefSeqiYP_392516.1. NC_007574.1.

Genome annotation databases

GeneIDi3773133.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X96697 mRNA. Translation: CAA65469.1 .
RefSeqi YP_392516.1. NC_007574.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3773133.

Family and domain databases

InterProi IPR011181. VP3_Rotav.
[Graphical view ]
Pfami PF06929. Rotavirus_VP3. 1 hit.
[Graphical view ]
PIRSFi PIRSF004015. LigT_rotavirus. 1 hit.
PROSITEi PS51589. SAM_MT56_VP3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiVP3_ROTHC
AccessioniPrimary (citable) accession number: Q82041
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3