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Q82041

- VP3_ROTHC

UniProt

Q82041 - VP3_ROTHC

Protein

Protein VP3

Gene
N/A
Organism
Rotavirus C (isolate Human/United Kingdom/Bristol/1989) (RV-C)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.By similarity

    Catalytic activityi

    GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
    S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
    3. mRNA guanylyltransferase activity Source: UniProtKB-EC
    4. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. viral process Source: InterPro

    Keywords - Molecular functioni

    Methyltransferase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    mRNA capping, mRNA processing

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding, RNA-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein VP3
    Including the following 2 domains:
    mRNA guanylyltransferase (EC:2.7.7.50)
    mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
    OrganismiRotavirus C (isolate Human/United Kingdom/Bristol/1989) (RV-C)
    Taxonomic identifieri31567 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000007664: Genome

    Subcellular locationi

    Virion Curated
    Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Potential.Curated

    GO - Cellular componenti

    1. viral nucleocapsid Source: InterPro

    Keywords - Cellular componenti

    Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 693693Protein VP3PRO_0000369872Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with VP1 Potential. Interacts with VP2.Curated

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the rotavirus VP3 family.Curated

    Family and domain databases

    InterProiIPR011181. VP3_Rotav.
    [Graphical view]
    PfamiPF06929. Rotavirus_VP3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004015. LigT_rotavirus. 1 hit.
    PROSITEiPS51589. SAM_MT56_VP3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q82041-1 [UniParc]FASTAAdd to Basket

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    MRVLGLFERG NNLNFADTYV YTWNQQYSYH ENAFLISNQV ATTIILYLDG    50
    ININEVNKAF ELLNSNGIPA LIIKPDHIGI FTSSNFTYDW QYKIVYFHEY 100
    TYYKNNEFIV SDEFWLYTNI NELLPYKILY YERGMRELYA GREYTLYNTA 150
    TDDDILYKYI YEKDSIMNGT DYKKLYDTNS VKNFVHFMRL LRMRFAVPFD 200
    QLSNRITRSR VFSKSRIHIG LRNESIPQAL DNIHSQWINY SANGIVISEL 250
    KGLGSYSEKK ISEFGIGQFK NYMNFLTLMF YIKNMKKKPS CTIIGAAPGY 300
    WISSMKKYFT IVTYDNKEVD STEHHNRYFT DDDIVNVKTN GVYIDVRSEF 350
    KTNDWRQRRK LIEEETIKWL EISYKLLENK RVEAILLKMT AMDGEIPDGY 400
    CVHSPTTYRK SEYYLLIDKH IIKRQKIKVT KSLMYNAINT IYSDNVFISG 450
    KYSLRGKTEG VLALYCLSNT INQKEKVIQY ANSFSGTCMT VRLNNTYEVD 500
    KIIDFKTNSD HTFLPSDFTC SLNTILTSYR GYAGIFGYAI TKDLKSNGNN 550
    HIYIIPNARD ENNFDTFGSH LGLSRYSHSK RFSESATTMS GYIFRDMVSG 600
    KENMQDTDKD NYASGHVFNA IAHYRFDYTY DIVGWLRLHK TGQFKVKSDI 650
    YKEHTDSEIR NAIESAYVYY LLDGDKVGEK YSKKMMEIWE VQV 693
    Length:693
    Mass (Da):81,316
    Last modified:November 1, 1996 - v1
    Checksum:iBD9AAF4E3533A175
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96697 mRNA. Translation: CAA65469.1.
    RefSeqiYP_392516.1. NC_007574.1.

    Genome annotation databases

    GeneIDi3773133.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96697 mRNA. Translation: CAA65469.1 .
    RefSeqi YP_392516.1. NC_007574.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3773133.

    Family and domain databases

    InterProi IPR011181. VP3_Rotav.
    [Graphical view ]
    Pfami PF06929. Rotavirus_VP3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004015. LigT_rotavirus. 1 hit.
    PROSITEi PS51589. SAM_MT56_VP3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiVP3_ROTHC
    AccessioniPrimary (citable) accession number: Q82041
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3