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Protein

Protein VP3

Gene
N/A
Organism
Rotavirus C (isolate Human/United Kingdom/Bristol/1989) (RV-C)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't (By similarity).By similarity

Catalytic activityi

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  3. mRNA guanylyltransferase activity Source: UniProtKB-EC
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. viral process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein VP3
Including the following 2 domains:
mRNA guanylyltransferase (EC:2.7.7.50)
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
OrganismiRotavirus C (isolate Human/United Kingdom/Bristol/1989) (RV-C)
Taxonomic identifieri31567 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007664: Genome

Subcellular locationi

Virion Curated
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging (Potential).Curated

GO - Cellular componenti

  1. viral nucleocapsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 693693Protein VP3PRO_0000369872Add
BLAST

Interactioni

Subunit structurei

Interacts with VP1 (Potential). Interacts with VP2.Curated

Family & Domainsi

Sequence similaritiesi

Belongs to the rotavirus VP3 family.Curated

Family and domain databases

InterProiIPR011181. VP3_Rotav.
[Graphical view]
PfamiPF06929. Rotavirus_VP3. 1 hit.
[Graphical view]
PIRSFiPIRSF004015. LigT_rotavirus. 1 hit.
PROSITEiPS51589. SAM_MT56_VP3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q82041-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLGLFERG NNLNFADTYV YTWNQQYSYH ENAFLISNQV ATTIILYLDG
60 70 80 90 100
ININEVNKAF ELLNSNGIPA LIIKPDHIGI FTSSNFTYDW QYKIVYFHEY
110 120 130 140 150
TYYKNNEFIV SDEFWLYTNI NELLPYKILY YERGMRELYA GREYTLYNTA
160 170 180 190 200
TDDDILYKYI YEKDSIMNGT DYKKLYDTNS VKNFVHFMRL LRMRFAVPFD
210 220 230 240 250
QLSNRITRSR VFSKSRIHIG LRNESIPQAL DNIHSQWINY SANGIVISEL
260 270 280 290 300
KGLGSYSEKK ISEFGIGQFK NYMNFLTLMF YIKNMKKKPS CTIIGAAPGY
310 320 330 340 350
WISSMKKYFT IVTYDNKEVD STEHHNRYFT DDDIVNVKTN GVYIDVRSEF
360 370 380 390 400
KTNDWRQRRK LIEEETIKWL EISYKLLENK RVEAILLKMT AMDGEIPDGY
410 420 430 440 450
CVHSPTTYRK SEYYLLIDKH IIKRQKIKVT KSLMYNAINT IYSDNVFISG
460 470 480 490 500
KYSLRGKTEG VLALYCLSNT INQKEKVIQY ANSFSGTCMT VRLNNTYEVD
510 520 530 540 550
KIIDFKTNSD HTFLPSDFTC SLNTILTSYR GYAGIFGYAI TKDLKSNGNN
560 570 580 590 600
HIYIIPNARD ENNFDTFGSH LGLSRYSHSK RFSESATTMS GYIFRDMVSG
610 620 630 640 650
KENMQDTDKD NYASGHVFNA IAHYRFDYTY DIVGWLRLHK TGQFKVKSDI
660 670 680 690
YKEHTDSEIR NAIESAYVYY LLDGDKVGEK YSKKMMEIWE VQV
Length:693
Mass (Da):81,316
Last modified:November 1, 1996 - v1
Checksum:iBD9AAF4E3533A175
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96697 mRNA. Translation: CAA65469.1.
RefSeqiYP_392516.1. NC_007574.1.

Genome annotation databases

GeneIDi3773133.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96697 mRNA. Translation: CAA65469.1.
RefSeqiYP_392516.1. NC_007574.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3773133.

Family and domain databases

InterProiIPR011181. VP3_Rotav.
[Graphical view]
PfamiPF06929. Rotavirus_VP3. 1 hit.
[Graphical view]
PIRSFiPIRSF004015. LigT_rotavirus. 1 hit.
PROSITEiPS51589. SAM_MT56_VP3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiVP3_ROTHC
AccessioniPrimary (citable) accession number: Q82041
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.