Reviewed, UniProtKB/Swiss-Prot Q82041 (VP3_ROTHC)

Names and origin

Protein names	Recommended name:     Protein VP3 Including the following 2 domains:     1- Recommended name:             mRNA guanylyltransferase               EC=2.7.7.50     2- Recommended name:             mRNA (guanine-N(7)-)-methyltransferase               EC=2.1.1.56
Organism	Rotavirus C (isolate Human/United Kingdom/Bristol/1989) (RV-C)
Taxonomic identifier	31567 [NCBI]
Taxonomic lineage	Viruses › dsRNA viruses › Reoviridae › Sedoreovirinae › Rotavirus
Virus host	Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	693 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.
Catalytic activity	GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA. S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with VP1 Potential. Interacts with VP2.
Subcellular location	Virion Potential. Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Potential.
Sequence similarities	Belongs to the rotavirus VP3 family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Core protein Virion
Ligand	GTP-binding Nucleotide-binding RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme Virus reference strain
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW viral reproduction Inferred from electronic annotation. Source: InterPro
Cellular component	viral nucleocapsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC mRNA guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 693	693	Protein VP3		PRO_0000369872

Sequences

	Sequence		Length	Mass (Da)
	Q82041-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BD9AAF4E3533A175	FASTA	693	81,316
	10 20 30 40 50 60 MRVLGLFERG NNLNFADTYV YTWNQQYSYH ENAFLISNQV ATTIILYLDG ININEVNKAF 70 80 90 100 110 120 ELLNSNGIPA LIIKPDHIGI FTSSNFTYDW QYKIVYFHEY TYYKNNEFIV SDEFWLYTNI 130 140 150 160 170 180 NELLPYKILY YERGMRELYA GREYTLYNTA TDDDILYKYI YEKDSIMNGT DYKKLYDTNS 190 200 210 220 230 240 VKNFVHFMRL LRMRFAVPFD QLSNRITRSR VFSKSRIHIG LRNESIPQAL DNIHSQWINY 250 260 270 280 290 300 SANGIVISEL KGLGSYSEKK ISEFGIGQFK NYMNFLTLMF YIKNMKKKPS CTIIGAAPGY 310 320 330 340 350 360 WISSMKKYFT IVTYDNKEVD STEHHNRYFT DDDIVNVKTN GVYIDVRSEF KTNDWRQRRK 370 380 390 400 410 420 LIEEETIKWL EISYKLLENK RVEAILLKMT AMDGEIPDGY CVHSPTTYRK SEYYLLIDKH 430 440 450 460 470 480 IIKRQKIKVT KSLMYNAINT IYSDNVFISG KYSLRGKTEG VLALYCLSNT INQKEKVIQY 490 500 510 520 530 540 ANSFSGTCMT VRLNNTYEVD KIIDFKTNSD HTFLPSDFTC SLNTILTSYR GYAGIFGYAI 550 560 570 580 590 600 TKDLKSNGNN HIYIIPNARD ENNFDTFGSH LGLSRYSHSK RFSESATTMS GYIFRDMVSG 610 620 630 640 650 660 KENMQDTDKD NYASGHVFNA IAHYRFDYTY DIVGWLRLHK TGQFKVKSDI YKEHTDSEIR 670 680 690 NAIESAYVYY LLDGDKVGEK YSKKMMEIWE VQV

References

[1]	"The VP3 gene of human group C rotavirus." Samarbaf-Zadeh A.R., Lambden P.R., Green S.M., Deng Y., Caul E.O., Clarke I.N. Virus Genes 13:169-173(1996) [PubMed: 8972570] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X96697 mRNA. Translation: CAA65469.1.
RefSeq	YP_392516.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3773133.
Family and domain databases
InterPro	IPR011181. Pesterase_Rotav. IPR009697. Rotavirus_VP3. [Graphical view]
Pfam	PF06929. Rotavirus_VP3. 1 hit. [Graphical view]
PIRSF	PIRSF004015. LigT_rotavirus. 1 hit.
ProtoNet	Search...

Entry information

Entry name

VP3_ROTHC

Accession

Primary (citable) accession number: Q82041

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 1, 1996
Last modified:	December 15, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)