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Q82040 (VP4_ROTHC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Outer capsid protein VP4
Alternative name(s):
Hemagglutinin

Cleaved into the following 2 chains:

  1. Outer capsid protein VP8*
  2. Outer capsid protein VP5*
OrganismRotavirus C (isolate Human/United Kingdom/Bristol/1989) (RV-C) [Reference proteome]
Taxonomic identifier31567 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length744 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors By similarity.

Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm By similarity.

VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies By similarity.

Subunit structure

VP4 is a homotrimer Potential.

Subcellular location

Outer capsid protein VP4: Virion. Host rough endoplasmic reticulum Potential. Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.

Outer capsid protein VP8*: Virion By similarity. Note: Outer capsid protein By similarity.

Outer capsid protein VP5*: Virion By similarity. Note: Outer capsid protein By similarity.

Post-translational modification

Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion By similarity.

Sequence similarities

Belongs to the rotavirus VP4 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 744744Outer capsid protein VP4
PRO_0000369876
Chain1 – 231231Outer capsid protein VP8* By similarity
PRO_0000369877
Chain248 – 744497Outer capsid protein VP5* By similarity
PRO_0000369878

Regions

Region401 – 42121Hydrophobic; possible role in virus entry into host cell Potential
Coiled coil496 – 53035 Potential

Sites

Site231 – 2322Cleavage Potential
Site247 – 2482Cleavage Potential

Amino acid modifications

Glycosylation391N-linked (GlcNAc...); by host Potential
Glycosylation1351N-linked (GlcNAc...); by host Potential
Glycosylation1761N-linked (GlcNAc...); by host Potential
Glycosylation1921N-linked (GlcNAc...); by host Potential
Glycosylation2611N-linked (GlcNAc...); by host Potential
Glycosylation3101N-linked (GlcNAc...); by host Potential
Glycosylation5971N-linked (GlcNAc...); by host Potential
Glycosylation6011N-linked (GlcNAc...); by host Potential
Glycosylation6391N-linked (GlcNAc...); by host Potential
Glycosylation6471N-linked (GlcNAc...); by host Potential

Sequences

Sequence LengthMass (Da)Tools
Q82040 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 96A9DBACBB7CD449

FASTA74484,081
        10         20         30         40         50         60 
MASSLYAQLI SQNYYSLGNE ILSDQQTNKV VSDYVDAGNY TYAQLPPTTW GSGSILKSAF 

        70         80         90        100        110        120 
STPEITGPHT NTVIEWSNLI NTNTWLLYQK PLNSVRLLKH GPDTYNSNLA AFELWYGKSG 

       130        140        150        160        170        180 
TTITSVYYNT INNQNKTHDA NSDCLILFWN EGSTQLEKQV VTFNWNVGGI LIKPINSSRM 

       190        200        210        220        230        240 
RICMSGMENF NNDSFNWENW NHEFPRSNPG ININMYTEYF LASSDPYTYL KNLQQPTAKT 

       250        260        270        280        290        300 
VDMKMMKKMN DNSKLGDGPI NVSNIISKDS LWQEVQYVRD ITLQCKILSE IVKGGGWGYD 

       310        320        330        340        350        360 
YTSVTFKTVN HTYSYTRAGE NVNAHVTISF NNVKERAYGG SLPTDFKIGR FDILDTDSYV 

       370        380        390        400        410        420 
YIDYWDDSEI FKNMVYVRDV RADIGGFQYS YSSEMSYYFQ IPVGSYPGLH SSRLQLVYDR 

       430        440        450        460        470        480 
CLLSQQFTDY AALNSLRFVF RVVSTSGWFI TTGDINTRRV ASGTGFAYSD GHVANTVGTI 

       490        500        510        520        530        540 
SFISLIPSNP NYQTPIASSS TVRMDLERKI NDLRDDFNAL ASSVALSDIL SLAMSPLTFS 

       550        560        570        580        590        600 
NLLESVPAIT SSVKDVAASV MKKFRSTKMF KKAAKQNYRE FVIGDLLEDV TNVARNNNSL 

       610        620        630        640        650        660 
NYSDITSAMM VSTTNRLQIT DVDTFSEIVS RSADNFISNR SYRMIENNTV HEITPTRRFS 

       670        680        690        700        710        720 
YDIKTLQQRN FDIDKFSKLA SQSPVISAIV DFATIKAIRD TYGISDDIIY KLVASDAPTI 

       730        740 
LSFINQNNPL IRNRITNLIN QCKL 

« Hide

References

[1]"Molecular characterization of the outer capsid spike protein (VP4) gene from human group C rotavirus."
Fielding P.A., Lambden P.R., Caul E.O., Clarke I.N.
Virology 204:442-446(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Whole genomic characterization of a human rotavirus strain B219 belonging to a novel group of the genus Rotavirus."
Nagashima S., Kobayashi N., Ishino M., Alam M.M., Ahmed M.U., Paul S.K., Ganesh B., Chawla-Sarkar M., Krishnan T., Naik T.N., Wang Y.-H.
J. Med. Virol. 80:2023-2033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PUTATIVE CLEAVAGE SITES.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X79442 Genomic RNA. Translation: CAA55958.1.
PIRS45061.
RefSeqYP_392514.1. NC_007572.1.

3D structure databases

ProteinModelPortalQ82040.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3773132.

Family and domain databases

InterProIPR000416. Haemagglutinin_VP4.
[Graphical view]
PfamPF00426. VP4_haemagglut. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameVP4_ROTHC
AccessionPrimary (citable) accession number: Q82040
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families