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Q82040

- VP4_ROTHC

UniProt

Q82040 - VP4_ROTHC

Protein

Outer capsid protein VP4

Gene
N/A
Organism
Rotavirus C (isolate Human/United Kingdom/Bristol/1989) (RV-C)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors By similarity.By similarity
    Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm By similarity.By similarity
    VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei231 – 2322CleavageSequence Analysis
    Sitei247 – 2482CleavageSequence Analysis

    GO - Biological processi

    1. permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW
    2. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hemagglutinin

    Keywords - Biological processi

    Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Viral penetration via permeabilization of host membrane, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Outer capsid protein VP4
    Alternative name(s):
    Hemagglutinin
    Cleaved into the following 2 chains:
    OrganismiRotavirus C (isolate Human/United Kingdom/Bristol/1989) (RV-C)
    Taxonomic identifieri31567 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000007664: Genome

    Subcellular locationi

    Chain Outer capsid protein VP4 : Virion. Host rough endoplasmic reticulum Curated
    Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.By similarity
    Chain Outer capsid protein VP8* : Virion By similarity
    Note: Outer capsid protein.By similarity
    Chain Outer capsid protein VP5* : Virion By similarity
    Note: Outer capsid protein.By similarity

    GO - Cellular componenti

    1. host cell rough endoplasmic reticulum Source: UniProtKB-SubCell
    2. viral outer capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host endoplasmic reticulum, Outer capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 744744Outer capsid protein VP4PRO_0000369876Add
    BLAST
    Chaini1 – 231231Outer capsid protein VP8*By similarityPRO_0000369877Add
    BLAST
    Chaini248 – 744497Outer capsid protein VP5*By similarityPRO_0000369878Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi39 – 391N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi135 – 1351N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi176 – 1761N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi192 – 1921N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi261 – 2611N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi310 – 3101N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi597 – 5971N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi601 – 6011N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi639 – 6391N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi647 – 6471N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion By similarity.By similarity

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    VP4 is a homotrimer.Curated

    Structurei

    3D structure databases

    ProteinModelPortaliQ82040.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni401 – 42121Hydrophobic; possible role in virus entry into host cellSequence AnalysisAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili496 – 53035Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the rotavirus VP4 family.Curated

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    InterProiIPR000416. Haemagglutinin_VP4.
    [Graphical view]
    PfamiPF00426. VP4_haemagglut. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q82040-1 [UniParc]FASTAAdd to Basket

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    MASSLYAQLI SQNYYSLGNE ILSDQQTNKV VSDYVDAGNY TYAQLPPTTW    50
    GSGSILKSAF STPEITGPHT NTVIEWSNLI NTNTWLLYQK PLNSVRLLKH 100
    GPDTYNSNLA AFELWYGKSG TTITSVYYNT INNQNKTHDA NSDCLILFWN 150
    EGSTQLEKQV VTFNWNVGGI LIKPINSSRM RICMSGMENF NNDSFNWENW 200
    NHEFPRSNPG ININMYTEYF LASSDPYTYL KNLQQPTAKT VDMKMMKKMN 250
    DNSKLGDGPI NVSNIISKDS LWQEVQYVRD ITLQCKILSE IVKGGGWGYD 300
    YTSVTFKTVN HTYSYTRAGE NVNAHVTISF NNVKERAYGG SLPTDFKIGR 350
    FDILDTDSYV YIDYWDDSEI FKNMVYVRDV RADIGGFQYS YSSEMSYYFQ 400
    IPVGSYPGLH SSRLQLVYDR CLLSQQFTDY AALNSLRFVF RVVSTSGWFI 450
    TTGDINTRRV ASGTGFAYSD GHVANTVGTI SFISLIPSNP NYQTPIASSS 500
    TVRMDLERKI NDLRDDFNAL ASSVALSDIL SLAMSPLTFS NLLESVPAIT 550
    SSVKDVAASV MKKFRSTKMF KKAAKQNYRE FVIGDLLEDV TNVARNNNSL 600
    NYSDITSAMM VSTTNRLQIT DVDTFSEIVS RSADNFISNR SYRMIENNTV 650
    HEITPTRRFS YDIKTLQQRN FDIDKFSKLA SQSPVISAIV DFATIKAIRD 700
    TYGISDDIIY KLVASDAPTI LSFINQNNPL IRNRITNLIN QCKL 744
    Length:744
    Mass (Da):84,081
    Last modified:November 1, 1996 - v1
    Checksum:i96A9DBACBB7CD449
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79442 Genomic RNA. Translation: CAA55958.1.
    PIRiS45061.
    RefSeqiYP_392514.1. NC_007572.1.

    Genome annotation databases

    GeneIDi3773132.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79442 Genomic RNA. Translation: CAA55958.1 .
    PIRi S45061.
    RefSeqi YP_392514.1. NC_007572.1.

    3D structure databases

    ProteinModelPortali Q82040.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3773132.

    Family and domain databases

    InterProi IPR000416. Haemagglutinin_VP4.
    [Graphical view ]
    Pfami PF00426. VP4_haemagglut. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of the outer capsid spike protein (VP4) gene from human group C rotavirus."
      Fielding P.A., Lambden P.R., Caul E.O., Clarke I.N.
      Virology 204:442-446(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Whole genomic characterization of a human rotavirus strain B219 belonging to a novel group of the genus Rotavirus."
      Nagashima S., Kobayashi N., Ishino M., Alam M.M., Ahmed M.U., Paul S.K., Ganesh B., Chawla-Sarkar M., Krishnan T., Naik T.N., Wang Y.-H.
      J. Med. Virol. 80:2023-2033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PUTATIVE CLEAVAGE SITES.

    Entry informationi

    Entry nameiVP4_ROTHC
    AccessioniPrimary (citable) accession number: Q82040
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3