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Q81ZU6 (GLGB_NITEU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:NE2029
OrganismNitrosomonas europaea (strain ATCC 19718 / NBRC 14298) [Complete proteome] [HAMAP]
Taxonomic identifier228410 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas

Protein attributes

Sequence length734 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7347341,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188720

Sites

Active site4131Nucleophile By similarity
Active site4661Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q81ZU6 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: AFB28499C3A49EE4

FASTA73484,102
        10         20         30         40         50         60 
MKSLSSSSPG SSEDSAQSLL LTARLYDPCS FLGMHAAPNG RLIRVFQPYM SRVWLHTLSG 

        70         80         90        100        110        120 
YQPMRSVHQD GIFEWEGEGV MHPYLLRMEN TATGVIEERH DPYAFPMQIS GHDLYLFNEG 

       130        140        150        160        170        180 
RLLQAYHMLG AHRVRNHGVT GTRFAVWAPN AERVSVVGDF NRWDGRVYPM MVHGHSGVWE 

       190        200        210        220        230        240 
LFIPDLPEGA IYKYEIRNRI SGEILLKTDP YATTYELRPN NAALTPIEQK YDWKDDDWIA 

       250        260        270        280        290        300 
RRKGWDWLHA PLNIYELHVG SWKRHPDGRF YSYHELADHL IPYLQDMGYS HVELLPISEH 

       310        320        330        340        350        360 
PLDESWGYQA TGYFAVTSRY GSPEAFMSFV DRCHQAGIGV ILDWVPAHFP QDSFSLARFD 

       370        380        390        400        410        420 
GTALYEHEDP RLGYHHDWGT YIFNYGRNEV KSFLLSSAHY WLSAFHIDGL RVDAVASMLY 

       430        440        450        460        470        480 
LNYSRKEGEW LPNRYGGHEN LEAIEFLRAL NTMVHGEFPG ALTFAEESTS WPAVSRPAYL 

       490        500        510        520        530        540 
GGLGFSMKWN MGWMNDTLSY MQHDPVHRRY HHNELTFNQL YAYTENFVLP LSHDEVVHGK 

       550        560        570        580        590        600 
KSMLDKMPGD GWQKFANLRL LFTYQMTCPG KKINFMGNEL GQGHEWRVGH ELDWYLLERD 

       610        620        630        640        650        660 
PHRGIQALTR DLNHLYLNTP ALHELDFFAE GFSWIDCHDV EQSVISYQRH ARDGSFVLVV 

       670        680        690        700        710        720 
LNFTPILRTG YRVGIPGSSA YQEVFNSDSI YYDGSNAGNA GKISPTGQPW SGQPDSIIIT 

       730 
LPPLAGVILK AADG 

« Hide

References

[1]"Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea."
Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.
J. Bacteriol. 185:2759-2773(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19718 / NBRC 14298.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL954747 Genomic DNA. Translation: CAD85940.1.
RefSeqNP_842039.1. NC_004757.1.

3D structure databases

ProteinModelPortalQ81ZU6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING228410.NE2029.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD85940; CAD85940; NE2029.
GeneID1082989.
KEGGneu:NE2029.
PATRIC22715383. VBINitEur56163_2275.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMALSYMQHD.
OrthoDBEOG6JX7GT.
PhylomeDBQ81ZU6.

Enzyme and pathway databases

BioCycNEUR228410:GJNO-2068-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_NITEU
AccessionPrimary (citable) accession number: Q81ZU6
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries