Reviewed, UniProtKB/Swiss-Prot Q81ZS7 (SYQ_NITEU)

Names and origin

Protein names

Recommended name:
    Glutaminyl-tRNA synthetase
    EC=6.1.1.18
Alternative name(s):
    Glutamine--tRNA ligase
      Short name=GlnRS

Gene names

Name:	glnS1
Ordered Locus Names:	NE2356
AND
Name:	glnS2
Ordered Locus Names:	NE2363

Organism

Nitrosomonas europaea [Complete proteome] [HAMAP]

Taxonomic identifier

915 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Nitrosomonadaceae › Nitrosomonas

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00126
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Glutaminyl-tRNA synthetase HAMAP MF_00126		PRO_0000195840
Regions
	Motif	34 – 44	11	"HIGH" region HAMAP MF_00126
	Motif	270 – 274	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	273	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81ZS7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2AAA9D841E04BF8C	FASTA	556	64,605
	10 20 30 40 50 60 MNTPSSPAHF IRNIIEEDNR TGKWNGRVET RFPPEPNGYL HIGHAKSICL NFGLALEYGG 70 80 90 100 110 120 VCHLRFDDTN PEKEAQEYVD AIIESVRWLG FDWKEHLYYA SDYFDQLYEF AEYLITQGKA 130 140 150 160 170 180 YVDSLSADEI RRLRGTLTEA GTNSPYRDRS AEENLDLFRR MRAGEFPDGV HVLRARIDMA 190 200 210 220 230 240 SPNINLRDPV IYRIRHIHHQ RTGDKWCIYP MYDYTHCISD ALERITHSLC TLEFEDHRPL 250 260 270 280 290 300 YDWVLDQLAE KIPCHPQQIE FARLNLTYSV MSKRKLIDLV ENKLVDGWND PRMNTLAGLR 310 320 330 340 350 360 RRGYTPESIR LFAERIGISK ADSWIDMTIL EDCLREDLNE RALRRIAVLD PVSLIIDNFP 370 380 390 400 410 420 DGHEETCYAP NHPQKPELGT RELRLTKQLY IDREDFMEIP NKGFFRLAPG AEVRLRYAFI 430 440 450 460 470 480 IKCTHVVKDD QGKILEIHCV YDPDTKSGTA GAETRKVRGN IHWLSATYAK AVEIRLYDRL 490 500 510 520 530 540 FIDSHPDTEG KDFKISLNPN SKEVITGYVE PSLCEAQPEQ RFQFERHGYF VADLADTGPG 550 KPIFNRTVSL RNTWKK

References

[1]

"Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea." Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J. J. Bacteriol. 185:2759-2773(2003) [PubMed: 12700255] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19718 / IFO 14298.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL954747 Genomic DNA. Translation: CAD86268.1. AL954747 Genomic DNA. Translation: CAD86275.1.
RefSeq	NP_842353.1. NP_842358.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1083323. 1083330.
GenomeReviews	Gene locus NE2356 in contig AL954747_GR. Gene locus NE2363 in contig AL954747_GR.
KEGG	neu:NE2356. neu:NE2363.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q81ZS7.
OMA	ILRMKQD.
Enzyme and pathway databases
BioCyc	NEUR228410:NE2356-MON. NEUR228410:NE2363-MON.
BRENDA	6.1.1.18. 444.
Family and domain databases
HAMAP	MF_00126. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ic. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ic_codon-bd. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 1 hit.
PANTHER	PTHR10119:SF3. GlnS. 1 hit. PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_NITEU

Accession

Primary (citable) accession number: Q81ZS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)