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Q81ZS7 (SYQ_NITEU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine--tRNA ligase

EC=6.1.1.18
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS
Gene names
Name:glnS1
Ordered Locus Names:NE2356
AND
Name:glnS2
Ordered Locus Names:NE2363
OrganismNitrosomonas europaea (strain ATCC 19718 / NBRC 14298) [Complete proteome] [HAMAP]
Taxonomic identifier228410 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas

Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00126

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00126.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutaminyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

glutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 556556Glutamine--tRNA ligase HAMAP-Rule MF_00126
PRO_0000195840

Regions

Motif34 – 4411"HIGH" region HAMAP-Rule MF_00126
Motif270 – 2745"KMSKS" region HAMAP-Rule MF_00126

Sites

Binding site2731ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q81ZS7 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 2AAA9D841E04BF8C

FASTA55664,605
        10         20         30         40         50         60 
MNTPSSPAHF IRNIIEEDNR TGKWNGRVET RFPPEPNGYL HIGHAKSICL NFGLALEYGG 

        70         80         90        100        110        120 
VCHLRFDDTN PEKEAQEYVD AIIESVRWLG FDWKEHLYYA SDYFDQLYEF AEYLITQGKA 

       130        140        150        160        170        180 
YVDSLSADEI RRLRGTLTEA GTNSPYRDRS AEENLDLFRR MRAGEFPDGV HVLRARIDMA 

       190        200        210        220        230        240 
SPNINLRDPV IYRIRHIHHQ RTGDKWCIYP MYDYTHCISD ALERITHSLC TLEFEDHRPL 

       250        260        270        280        290        300 
YDWVLDQLAE KIPCHPQQIE FARLNLTYSV MSKRKLIDLV ENKLVDGWND PRMNTLAGLR 

       310        320        330        340        350        360 
RRGYTPESIR LFAERIGISK ADSWIDMTIL EDCLREDLNE RALRRIAVLD PVSLIIDNFP 

       370        380        390        400        410        420 
DGHEETCYAP NHPQKPELGT RELRLTKQLY IDREDFMEIP NKGFFRLAPG AEVRLRYAFI 

       430        440        450        460        470        480 
IKCTHVVKDD QGKILEIHCV YDPDTKSGTA GAETRKVRGN IHWLSATYAK AVEIRLYDRL 

       490        500        510        520        530        540 
FIDSHPDTEG KDFKISLNPN SKEVITGYVE PSLCEAQPEQ RFQFERHGYF VADLADTGPG 

       550 
KPIFNRTVSL RNTWKK 

« Hide

References

[1]"Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea."
Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.
J. Bacteriol. 185:2759-2773(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19718 / NBRC 14298.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL954747 Genomic DNA. Translation: CAD86268.1.
AL954747 Genomic DNA. Translation: CAD86275.1.
RefSeqNP_842353.1. NC_004757.1.
NP_842358.1. NC_004757.1.

3D structure databases

ProteinModelPortalQ81ZS7.
SMRQ81ZS7. Positions 9-553.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING228410.NE2363.

Proteomic databases

PRIDEQ81ZS7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD86268; CAD86268; NE2356.
CAD86275; CAD86275; NE2363.
GeneID1083323.
1083330.
KEGGneu:NE2356.
neu:NE2363.
PATRIC22716122. VBINitEur56163_2638.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000259232.
KOK01886.
OMAKSDSWID.
OrthoDBEOG6DRPF7.
PhylomeDBQ81ZS7.

Enzyme and pathway databases

BioCycNEUR228410:GJNO-2401-MONOMER.
NEUR228410:GJNO-2408-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPMF_00126. Gln_tRNA_synth.
InterProIPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00440. glnS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYQ_NITEU
AccessionPrimary (citable) accession number: Q81ZS7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries