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Q81ZS7

- SYQ_NITEU

UniProt

Q81ZS7 - SYQ_NITEU

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Protein
Glutamine--tRNA ligase
Gene
glnS1, NE2356
glnS2, NE2363
Organism
Nitrosomonas europaea (strain ATCC 19718 / NBRC 14298)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei273 – 2731ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glutaminyl-tRNA aminoacylation Source: InterPro
  2. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNEUR228410:GJNO-2401-MONOMER.
NEUR228410:GJNO-2408-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--tRNA ligase (EC:6.1.1.18)
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name:
GlnRS
Gene namesi
Name:glnS1
Ordered Locus Names:NE2356
AND
Name:glnS2
Ordered Locus Names:NE2363
OrganismiNitrosomonas europaea (strain ATCC 19718 / NBRC 14298)
Taxonomic identifieri228410 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas
ProteomesiUP000001416: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Glutamine--tRNA ligaseUniRule annotation
PRO_0000195840Add
BLAST

Proteomic databases

PRIDEiQ81ZS7.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi228410.NE2363.

Structurei

3D structure databases

ProteinModelPortaliQ81ZS7.
SMRiQ81ZS7. Positions 9-553.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi34 – 4411"HIGH" regionUniRule annotation
Add
BLAST
Motifi270 – 2745"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000259232.
KOiK01886.
OMAiKSDSWID.
OrthoDBiEOG6DRPF7.
PhylomeDBiQ81ZS7.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPiMF_00126. Gln_tRNA_synth.
InterProiIPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81ZS7-1 [UniParc]FASTAAdd to Basket

« Hide

MNTPSSPAHF IRNIIEEDNR TGKWNGRVET RFPPEPNGYL HIGHAKSICL    50
NFGLALEYGG VCHLRFDDTN PEKEAQEYVD AIIESVRWLG FDWKEHLYYA 100
SDYFDQLYEF AEYLITQGKA YVDSLSADEI RRLRGTLTEA GTNSPYRDRS 150
AEENLDLFRR MRAGEFPDGV HVLRARIDMA SPNINLRDPV IYRIRHIHHQ 200
RTGDKWCIYP MYDYTHCISD ALERITHSLC TLEFEDHRPL YDWVLDQLAE 250
KIPCHPQQIE FARLNLTYSV MSKRKLIDLV ENKLVDGWND PRMNTLAGLR 300
RRGYTPESIR LFAERIGISK ADSWIDMTIL EDCLREDLNE RALRRIAVLD 350
PVSLIIDNFP DGHEETCYAP NHPQKPELGT RELRLTKQLY IDREDFMEIP 400
NKGFFRLAPG AEVRLRYAFI IKCTHVVKDD QGKILEIHCV YDPDTKSGTA 450
GAETRKVRGN IHWLSATYAK AVEIRLYDRL FIDSHPDTEG KDFKISLNPN 500
SKEVITGYVE PSLCEAQPEQ RFQFERHGYF VADLADTGPG KPIFNRTVSL 550
RNTWKK 556
Length:556
Mass (Da):64,605
Last modified:June 1, 2003 - v1
Checksum:i2AAA9D841E04BF8C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL954747 Genomic DNA. Translation: CAD86268.1.
AL954747 Genomic DNA. Translation: CAD86275.1.
RefSeqiNP_842353.1. NC_004757.1.
NP_842358.1. NC_004757.1.
WP_011112836.1. NC_004757.1.

Genome annotation databases

EnsemblBacteriaiCAD86268; CAD86268; NE2356.
CAD86275; CAD86275; NE2363.
GeneIDi1083323.
1083330.
KEGGineu:NE2356.
neu:NE2363.
PATRICi22716122. VBINitEur56163_2638.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL954747 Genomic DNA. Translation: CAD86268.1 .
AL954747 Genomic DNA. Translation: CAD86275.1 .
RefSeqi NP_842353.1. NC_004757.1.
NP_842358.1. NC_004757.1.
WP_011112836.1. NC_004757.1.

3D structure databases

ProteinModelPortali Q81ZS7.
SMRi Q81ZS7. Positions 9-553.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 228410.NE2363.

Proteomic databases

PRIDEi Q81ZS7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD86268 ; CAD86268 ; NE2356 .
CAD86275 ; CAD86275 ; NE2363 .
GeneIDi 1083323.
1083330.
KEGGi neu:NE2356.
neu:NE2363.
PATRICi 22716122. VBINitEur56163_2638.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000259232.
KOi K01886.
OMAi KSDSWID.
OrthoDBi EOG6DRPF7.
PhylomeDBi Q81ZS7.

Enzyme and pathway databases

BioCyci NEUR228410:GJNO-2401-MONOMER.
NEUR228410:GJNO-2408-MONOMER.

Family and domain databases

Gene3Di 1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPi MF_00126. Gln_tRNA_synth.
InterProi IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF50715. SSF50715. 1 hit.
TIGRFAMsi TIGR00440. glnS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea."
    Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.
    J. Bacteriol. 185:2759-2773(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 19718 / NBRC 14298.

Entry informationi

Entry nameiSYQ_NITEU
AccessioniPrimary (citable) accession number: Q81ZS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2003
Last modified: September 3, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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