Q81ZS3 (EFTU_NITEU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor Tu
Short name=EF-Tu

Gene names

Name:	tuf1
Ordered Locus Names:	NE0399
AND
Name:	tuf2
Ordered Locus Names:	NE2052

Organism

Nitrosomonas europaea (strain ATCC 19718 / NBRC 14298) [Complete proteome] [HAMAP]

Taxonomic identifier

228410 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Nitrosomonadaceae › Nitrosomonas › Nitrosomonas europaea

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis By similarity. HAMAP-Rule MF_00118
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Elongation factor Tu HAMAP-Rule MF_00118		PRO_0000337444
Regions
	Nucleotide binding	19 – 26	8	GTP By similarity
	Nucleotide binding	81 – 85	5	GTP By similarity
	Nucleotide binding	136 – 139	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81ZS3 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 31E5873E187EE86B	FASTA	396	42,928
	10 20 30 40 50 60 MAKSKFERVK PHVNVGTIGH VDHGKTTLTA AITTILTKKF GGEAKSYDQI DSAPEERARG 70 80 90 100 110 120 ITINTSHVEY ETDKRHYAHV DCPGHADYVK NMITGAAQMD GAILVVSAAD GPMPQTREHI 130 140 150 160 170 180 LLARQVGVPY IIVFMNKADM VDDAELLELV EMEIRELLSN YDFPGDDTPI IIGSALKALE 190 200 210 220 230 240 GDKSDIGEAA ILKLAEALDS YIPEPERAID GAFIMPVEDV FSISGRGTVV TGRVERGIVK 250 260 270 280 290 300 VGDEIEIVGL KPTIKTVCTG VEMFRKLLDQ GQAGDNVGIL LRGTKREEVE RGQVLAKPGS 310 320 330 340 350 360 ILPHTKFTAE IYVLSKEEGG RHTPFFAGYR PQFYFRTTDV TGSIELPAGV EMVMPGDNIS 370 380 390 VTVNLIAPIA MDEGLRFAIR EGGRTVGAGV VAKVIE

References

[1]

"Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea." Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J. J. Bacteriol. 185:2759-2773(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19718 / NBRC 14298.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL954747 Genomic DNA. Translation: CAD84310.1. AL954747 Genomic DNA. Translation: CAD85963.1.
RefSeq	NP_840486.1. NC_004757.1. NP_842062.1. NC_004757.1.
3D structure databases
HSSP	HSSP built from PDB template 1XB2 based on UniProtKB P49410.
ProteinModelPortal	Q81ZS3.
SMR	Q81ZS3. Positions 2-395.
ModBase	Search...
Protein-protein interaction databases
STRING	228410.NE2052.
Proteomic databases
PRIDE	Q81ZS3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD84310; CAD84310; NE0399. CAD85963; CAD85963; NE2052.
GeneID	1081335. 1083013.
KEGG	neu:NE0399. neu:NE2052.
PATRIC	22711669. VBINitEur56163_0442.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0050.
HOGENOM	HOG000229290.
KO	K02358.
OMA	CEFVGYN.
ProtClustDB	PRK00049.
Enzyme and pathway databases
BioCyc	NEUR228410:GJNO-2092-MONOMER. NEUR228410:GJNO-416-MONOMER.
Family and domain databases
HAMAP	MF_00118_B. EF_Tu_B.
InterPro	IPR000795. EF_GTP-bd_dom. IPR005225. Small_GTP-bd_dom. IPR009001. Transl_elong_EF1A/Init_IF2_C. IPR004161. Transl_elong_EFTu/EF1A_2. IPR004541. Transl_elong_EFTu/EF1A_bac/org. IPR004160. Transl_elong_EFTu/EF1A_C. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF03143. GTP_EFTU_D3. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF50465. Elong_init_C. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00485. EF-Tu. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFTU_NITEU

Accession

Primary (citable) accession number: Q81ZS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program