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Protein

N5-carboxyaminoimidazole ribonucleotide mutase

Gene

purE

Organism
Bacillus anthracis
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).UniRule annotation

Catalytic activityi

5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101Acetate 1; via carbonyl oxygenCombined sources
Binding sitei11 – 111SubstrateUniRule annotation
Binding sitei14 – 141Acetate 2Combined sources
Binding sitei14 – 141SubstrateUniRule annotation
Binding sitei41 – 411SubstrateUniRule annotation
Binding sitei66 – 661Acetate 2; via amide nitrogenCombined sources
Binding sitei94 – 941Acetate 4Combined sources

GO - Molecular functioni

  1. 5-(carboxyamino)imidazole ribonucleotide mutase activity Source: UniProtKB-EC
  2. lyase activity Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

IsomeraseUniRule annotation, LyaseImported

Keywords - Biological processi

Purine biosynthesisUniRule annotation

Enzyme and pathway databases

BioCyciANTHRA:PURE-MONOMER.
BANT260799:GJAJ-317-MONOMER.
BANT261594:GJ7F-326-MONOMER.
UniPathwayiUPA00074; UER00943.

Names & Taxonomyi

Protein namesi
Recommended name:
N5-carboxyaminoimidazole ribonucleotide mutaseUniRule annotation (EC:5.4.99.18UniRule annotation)
Short name:
N5-CAIR mutaseUniRule annotation
Gene namesi
Name:purEImported
Ordered Locus Names:BA_0288Imported, BAS0275Imported, GBAA_0288Imported
ORF Names:BACvac02_0349Imported, BAHan_0354Imported, DH23_0272Imported, DJ42_4802Imported, DJ43_4834Imported, DJ44_4148Imported, DJ45_5599Imported, DJ48_3919Imported, DJ49_5206Imported, HYU01_01580Imported, NX97_02285Imported, OY21_21055Imported, OY22_04990Imported, OY23_03375Imported, OY24_20135Imported, OY25_00660Imported, OY26_06910Imported, OY27_03650Imported, OY28_08860Imported, OY29_02195Imported, OY30_03455Imported, OY32_02560Imported, OY34_01335Imported, OY35_02495Imported, OY36_18070Imported, OY37_01270Imported, OY38_08675Imported, OY39_02940Imported, OY40_03995Imported
OrganismiBacillus anthracisImported
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000000427: Chromosome, UP000000594: Chromosome, UP000005639: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi198094.BA_0288.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XMPX-ray1.80A/B/C/D/E/F/G/H1-161[»]
4AY3X-ray1.76A/B/C/D1-161[»]
4AY4X-ray2.00A/B/C/D1-161[»]
4B4KX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L1-161[»]
ProteinModelPortaliQ81ZH8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ81ZH8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni38 – 414Acetate 1 bindingCombined sources
Regioni38 – 414Acetate 3 bindingCombined sources

Sequence similaritiesi

Belongs to the AIR carboxylase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000034141.
KOiK01588.
OMAiSDWATMR.
OrthoDBiEOG6Z0QH2.

Family and domain databases

Gene3Di3.40.50.7700. 1 hit.
InterProiIPR024694. N5-CAIR_mutase_PurE.
IPR000031. PurE_dom.
[Graphical view]
PfamiPF00731. AIRC. 1 hit.
[Graphical view]
PIRSFiPIRSF001338. AIR_carboxylase. 1 hit.
SMARTiSM01001. AIRC. 1 hit.
[Graphical view]
SUPFAMiSSF52255. SSF52255. 1 hit.
TIGRFAMsiTIGR01162. purE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q81ZH8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSLVGVIMG STSDWETMKY ACDILDELNI PYEKKVVSAH RTPDYMFEYA
60 70 80 90 100
ETARERGLKV IIAGAGGAAH LPGMVAAKTN LPVIGVPVQS KALNGLDSLL
110 120 130 140 150
SIVQMPGGVP VATVAIGKAG STNAGLLAAQ ILGSFHDDIH DALELRREAI
160
EKDVREGSEL V
Length:161
Mass (Da):17,042
Last modified:June 1, 2003 - v1
Checksum:i6BC2A80C2A91A94E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP24324.1.
AE017334 Genomic DNA. Translation: AAT29375.1.
AE017225 Genomic DNA. Translation: AAT52606.1.
CP008846 Genomic DNA. Translation: AIF54757.1.
CP007660 Genomic DNA. Translation: AIK06398.1.
CP007618 Genomic DNA. Translation: AIK30710.1.
CP008752 Genomic DNA. Translation: AIK50938.1.
CP007704 Genomic DNA. Translation: AIK60423.1.
CP008853 Genomic DNA. Translation: AIM04255.1.
CP008854 Genomic DNA. Translation: AIM09640.1.
JNOD01000011 Genomic DNA. Translation: KFJ82979.1.
JMPV01000005 Genomic DNA. Translation: KFL76696.1.
JMPU01000011 Genomic DNA. Translation: KFL79633.1.
JTAQ01000029 Genomic DNA. Translation: KGZ45687.1.
JTAR01000018 Genomic DNA. Translation: KGZ54553.1.
JTAS01000005 Genomic DNA. Translation: KGZ57371.1.
JSZQ01000042 Genomic DNA. Translation: KGZ65779.1.
JSZR01000003 Genomic DNA. Translation: KGZ72786.1.
JSZP01000012 Genomic DNA. Translation: KGZ73138.1.
JSZS01000029 Genomic DNA. Translation: KGZ85676.1.
JSZU01000021 Genomic DNA. Translation: KGZ86679.1.
JSZT01000017 Genomic DNA. Translation: KGZ88850.1.
JSZW01000031 Genomic DNA. Translation: KHA04014.1.
JSZV01000006 Genomic DNA. Translation: KHA05722.1.
JSZY01000010 Genomic DNA. Translation: KHA07840.1.
JTAC01000051 Genomic DNA. Translation: KHA19246.1.
JTAA01000004 Genomic DNA. Translation: KHA19459.1.
JTAB01000008 Genomic DNA. Translation: KHA20383.1.
JTAE01000015 Genomic DNA. Translation: KHA34279.1.
JTAD01000006 Genomic DNA. Translation: KHA34286.1.
JTAF01000014 Genomic DNA. Translation: KHA43999.1.
JTAG01000024 Genomic DNA. Translation: KHA45129.1.
RefSeqiNP_842838.1. NC_003997.3.
YP_016900.1. NC_007530.2.
YP_026555.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP24324; AAP24324; BA_0288.
AAT29375; AAT29375; GBAA_0288.
AAT52606; AAT52606; BAS0275.
GeneIDi1085644.
2817097.
2851322.
KEGGiban:BA_0288.
bar:GBAA_0288.
bat:BAS0275.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP24324.1.
AE017334 Genomic DNA. Translation: AAT29375.1.
AE017225 Genomic DNA. Translation: AAT52606.1.
CP008846 Genomic DNA. Translation: AIF54757.1.
CP007660 Genomic DNA. Translation: AIK06398.1.
CP007618 Genomic DNA. Translation: AIK30710.1.
CP008752 Genomic DNA. Translation: AIK50938.1.
CP007704 Genomic DNA. Translation: AIK60423.1.
CP008853 Genomic DNA. Translation: AIM04255.1.
CP008854 Genomic DNA. Translation: AIM09640.1.
JNOD01000011 Genomic DNA. Translation: KFJ82979.1.
JMPV01000005 Genomic DNA. Translation: KFL76696.1.
JMPU01000011 Genomic DNA. Translation: KFL79633.1.
JTAQ01000029 Genomic DNA. Translation: KGZ45687.1.
JTAR01000018 Genomic DNA. Translation: KGZ54553.1.
JTAS01000005 Genomic DNA. Translation: KGZ57371.1.
JSZQ01000042 Genomic DNA. Translation: KGZ65779.1.
JSZR01000003 Genomic DNA. Translation: KGZ72786.1.
JSZP01000012 Genomic DNA. Translation: KGZ73138.1.
JSZS01000029 Genomic DNA. Translation: KGZ85676.1.
JSZU01000021 Genomic DNA. Translation: KGZ86679.1.
JSZT01000017 Genomic DNA. Translation: KGZ88850.1.
JSZW01000031 Genomic DNA. Translation: KHA04014.1.
JSZV01000006 Genomic DNA. Translation: KHA05722.1.
JSZY01000010 Genomic DNA. Translation: KHA07840.1.
JTAC01000051 Genomic DNA. Translation: KHA19246.1.
JTAA01000004 Genomic DNA. Translation: KHA19459.1.
JTAB01000008 Genomic DNA. Translation: KHA20383.1.
JTAE01000015 Genomic DNA. Translation: KHA34279.1.
JTAD01000006 Genomic DNA. Translation: KHA34286.1.
JTAF01000014 Genomic DNA. Translation: KHA43999.1.
JTAG01000024 Genomic DNA. Translation: KHA45129.1.
RefSeqiNP_842838.1. NC_003997.3.
YP_016900.1. NC_007530.2.
YP_026555.1. NC_005945.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XMPX-ray1.80A/B/C/D/E/F/G/H1-161[»]
4AY3X-ray1.76A/B/C/D1-161[»]
4AY4X-ray2.00A/B/C/D1-161[»]
4B4KX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L1-161[»]
ProteinModelPortaliQ81ZH8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198094.BA_0288.

Protocols and materials databases

DNASUi1085644.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP24324; AAP24324; BA_0288.
AAT29375; AAT29375; GBAA_0288.
AAT52606; AAT52606; BAS0275.
GeneIDi1085644.
2817097.
2851322.
KEGGiban:BA_0288.
bar:GBAA_0288.
bat:BAS0275.

Phylogenomic databases

HOGENOMiHOG000034141.
KOiK01588.
OMAiSDWATMR.
OrthoDBiEOG6Z0QH2.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00943.
BioCyciANTHRA:PURE-MONOMER.
BANT260799:GJAJ-317-MONOMER.
BANT261594:GJ7F-326-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ81ZH8.

Family and domain databases

Gene3Di3.40.50.7700. 1 hit.
InterProiIPR024694. N5-CAIR_mutase_PurE.
IPR000031. PurE_dom.
[Graphical view]
PfamiPF00731. AIRC. 1 hit.
[Graphical view]
PIRSFiPIRSF001338. AIR_carboxylase. 1 hit.
SMARTiSM01001. AIRC. 1 hit.
[Graphical view]
SUPFAMiSSF52255. SSF52255. 1 hit.
TIGRFAMsiTIGR01162. purE. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
    Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
    , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
    Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AmesImported and Ames / isolate PortonImported.
  2. "Complete genome sequence of Bacillus anthracis Sterne."
    Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SterneImported.
  3. "Crystal structure of PurE (BA0288) from Bacillus anthracis at 1.8 A resolution."
    Boyle M.P., Kalliomaa A.K., Levdikov V., Blagova E., Fogg M.J., Brannigan J.A., Wilson K.S., Wilkinson A.J.
    Proteins 61:674-676(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS).
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames AncestorImported and Ames ancestorImported.
  5. Joardar V., Shrivastava S., Brinkac L.M., Harkins D.M., Durkin A.S., Sutton G.
    Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: AmesImported.
  6. "Elastic and inelastic diffraction changes upon variation of the relative humidity environment of PurE crystals."
    Oliete R., Pous J., Rodriguez-Puente S., Abad-Zapatero C., Guasch A.
    Acta Crystallogr. D 69:194-212(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.76 ANGSTROMS).
  7. Tong Y.G., Huang Y., An X.P., Li S.S., Mi Z.Q., Pei G.Q., Zhang Z.Y., Fan H.
    Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Cvac02Imported and HanImported.
  8. "Complete Genome Sequence of Bacillus anthracis HYU01, Isolated from Soil Samples in the Korean Peninsula."
    Kim S.K., Chung W.H., Kim S.H., Jung K.H., Kim N., Chai Y.G.
    Genome Announc. 2:e00769-14(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: HYU01Imported.
  9. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 2000031021Imported, A.Br.003Imported, Smith 1013Imported and Zimbabwe 89Imported.
  10. "Draft Genome Sequence of Bacillus anthracis STI."
    Okinaka R.T., Challacombe J.F., Drees K., Birdsell D.N., Janke N., Naumann A., Seymour M., O'Neill H.H., Schupp J., Sahl J., Foster J.T., Pearson T., Keim P.
    Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: STI-1Imported.
  11. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: BFVImported.
  12. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Delta SterneImported.
  13. "Centers for Disease Control and Prevention Bacillus anthracis whole genome sequencing."
    Hoffmaster A.R., Marston C.K., Gee J., Tiller R., Beesley C., Bell M., Elrod M., France M., Sammons S., Rodriguez-R L.M., Weigand M.R., Konstantinidis K.T.
    Submitted (NOV-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 2000031027Imported.
  14. "Centers for Disease Control and Prevention Bacillus anthracis whole genome sequencing."
    Hoffmaster A.R., Marston C.K., Gee J., Tiller R., Beesley C.A., Bell M., Elrod M., France M., Sammons S., Rodriguez-R L.M., Weigand M.R., Konstantinidis K.T.
    Submitted (NOV-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 2000031006Imported, 2000031023Imported, 2000031031Imported, 2000031038Imported, 2000031039Imported, 2000031052Imported, 2000031075Imported, 2000031709Imported, 2000031765Imported, 2000032819Imported, 2000032879Imported, 2000032951Imported, 2000032967Imported, 2000032968Imported, 2000032975Imported, 2000032979Imported, 2000032989Imported and 2002013094Imported.

Entry informationi

Entry nameiQ81ZH8_BACAN
AccessioniPrimary (citable) accession number: Q81ZH8
Secondary accession number(s): E9RB02
, E9RB03, Q6I4C5, Q6KY27
Entry historyi
Integrated into UniProtKB/TrEMBL: June 1, 2003
Last sequence update: June 1, 2003
Last modified: February 4, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.