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Protein

N5-carboxyaminoimidazole ribonucleotide synthase

Gene

purK

Organism
Bacillus anthracis
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO3- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).UniRule annotation
Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO3- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).UniRule annotation

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. N5-carboxyaminoimidazole ribonucleotide synthase (purK), N5-carboxyaminoimidazole ribonucleotide synthase (purK), N5-carboxyaminoimidazole ribonucleotide synthase (purK), N5-carboxyaminoimidazole ribonucleotide synthase (purK), N5-carboxyaminoimidazole ribonucleotide synthase (purK), N5-carboxyaminoimidazole ribonucleotide synthase (purK)
  2. N5-carboxyaminoimidazole ribonucleotide mutase (purE), N5-carboxyaminoimidazole ribonucleotide mutase (purE), N5-carboxyaminoimidazole ribonucleotide mutase (purE), N5-carboxyaminoimidazole ribonucleotide mutase (purE)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei76ATP; via carbonyl oxygenCombined sources1
Binding sitei107ATPUniRule annotationCombined sources1
Binding sitei147ATPUniRule annotationCombined sources1
Binding sitei190ATPUniRule annotationCombined sources1
Binding sitei213ATPUniRule annotation1
Metal bindingi255CalciumCombined sources1
Metal bindingi268CalciumCombined sources1
Binding sitei340ATPCombined sources1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi152 – 158ATPUniRule annotation7
Nucleotide bindingi153 – 158ATPCombined sources6
Nucleotide bindingi182 – 185ATPUniRule annotationCombined sources4
Nucleotide bindingi213 – 216ATPCombined sources4
Nucleotide bindingi267 – 268ATPUniRule annotationCombined sources2
Nucleotide bindingi272 – 275ATPCombined sources4
Nucleotide bindingi347 – 348ATPCombined sources2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotation, LyaseImported
Biological processPurine biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, CalciumCombined sources, Metal-bindingCombined sources, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00942.

Names & Taxonomyi

Protein namesi
Recommended name:
N5-carboxyaminoimidazole ribonucleotide synthaseUniRule annotation (EC:6.3.4.18UniRule annotation)
Short name:
N5-CAIR synthaseUniRule annotation
Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide synthetaseUniRule annotation
Gene namesi
Name:purKUniRule annotationImported
Ordered Locus Names:GBAA_0289Imported
ORF Names:BASH2_05529Imported
OrganismiBacillus anthracisImported
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi260799.BAS0276.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DLKX-ray2.02A/B1-380[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini111 – 297ATP-graspInterPro annotationAdd BLAST187

Sequence similaritiesi

Belongs to the PurK/PurT family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CY8. Bacteria.
COG0026. LUCA.
HOGENOMiHOG000034029.
KOiK01589.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
HAMAPiMF_01928. PurK. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR006140. D-isomer_DH_NAD-bd.
IPR016185. PreATP-grasp_dom_sf.
IPR005875. PurK.
IPR011054. Rudment_hybrid_motif.
PfamiView protein in Pfam
PF02826. 2-Hacid_dh_C. 1 hit.
PF02222. ATP-grasp. 1 hit.
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01161. purK. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q81ZH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRIILPGKT IGIIGGGQLG RMMALAAKEM GYKIAVLDPT KNSPCAQVAD
60 70 80 90 100
IEIVASYDDL KAIQHLAEIS DVVTYEFENI DYRCLQWLEK HAYLPQGSQL
110 120 130 140 150
LSKTQNRFTE KNAIEKAGLP VATYRLVQNQ EQLTEAIAEL SYPSVLKTTT
160 170 180 190 200
GGYDGKGQVV LRSEADVDEA RKLANAAECI LEKWVPFEKE VSVIVIRSVS
210 220 230 240 250
GETKVFPVAE NIHVNNILHE SIVPARITEE LSQKAIAYAK VLADELELVG
260 270 280 290 300
TLAVEMFATA DGEIYINELA PRPHNSGHYT QDACETSQFG QHIRAICNLP
310 320 330 340 350
LGETNLLKPV VMVNILGEHI EGVLRQVNRL TGCYLHLYGK EEAKAQRKMG
360 370 380
HVNILNDNIE VALEKAKSLH IWDHQEQLLE GKR
Length:383
Mass (Da):42,517
Last modified:June 1, 2003 - v1
Checksum:iBE0E5E1A56F88287
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017334 Genomic DNA. Translation: AAT29376.2.
AP014833 Genomic DNA. Translation: BAR78936.1.
RefSeqiWP_000196795.1. NZ_MVOA01000002.1.

Genome annotation databases

EnsemblBacteriaiAAT29376; AAT29376; GBAA_0289.
ANH84699; ANH84699; A8C77_01640.
KEGGibanh:HYU01_01585.
bar:GBAA_0289.
PATRICifig|1392.230.peg.276.

Similar proteinsi

Entry informationi

Entry nameiQ81ZH7_BACAN
AccessioniPrimary (citable) accession number: Q81ZH7
Secondary accession number(s): E9QVL4
, E9QVL5, Q6I4C4, Q6KY26
Entry historyiIntegrated into UniProtKB/TrEMBL: June 1, 2003
Last sequence update: June 1, 2003
Last modified: November 22, 2017
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported