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Protein

N5-carboxyaminoimidazole ribonucleotide synthase

Gene

purK

Organism
Bacillus anthracis
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO3- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).UniRule annotation
Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).UniRule annotation

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei107 – 1071ATPUniRule annotation
Binding sitei147 – 1471ATPUniRule annotation
Binding sitei190 – 1901ATPUniRule annotation
Binding sitei213 – 2131ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi152 – 1587ATPUniRule annotation
Nucleotide bindingi182 – 1854ATPUniRule annotation
Nucleotide bindingi267 – 2682ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ligase activity Source: UniProtKB-KW
  3. metal ion binding Source: InterPro
  4. NAD binding Source: InterPro
  5. phosphoribosylaminoimidazole carboxylase activity Source: UniProtKB-EC

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. oxidation-reduction process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation, LyaseImported

Keywords - Biological processi

Purine biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciANTHRA:PURK-MONOMER.
BANT260799:GJAJ-318-MONOMER.
BANT261594:GJ7F-327-MONOMER.
UniPathwayiUPA00074; UER00942.

Names & Taxonomyi

Protein namesi
Recommended name:
N5-carboxyaminoimidazole ribonucleotide synthaseUniRule annotation (EC:6.3.4.18UniRule annotation)
Short name:
N5-CAIR synthaseUniRule annotation
Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide synthetaseUniRule annotation
Gene namesi
Name:purKUniRule annotationImported
Ordered Locus Names:BA_0289Imported, BAS0276Imported, GBAA_0289Imported
ORF Names:TM00_01640Imported
OrganismiBacillus anthracisImported
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000005639 Componenti: Chromosome UP000000594 Componenti: Chromosome UP000000427 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi198094.BA_0289.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini111 – 297187ATP-graspUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PurK/PurT family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000034029.
KOiK01589.
OMAiDSPCGQV.
OrthoDBiEOG6QZMX7.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01928. PurK.
InterProiIPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006140. D-isomer_DH_NAD-bd.
IPR016185. PreATP-grasp_dom.
IPR005875. PurK.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF02826. 2-Hacid_dh_C. 1 hit.
PF02222. ATP-grasp. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01161. purK. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81ZH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRIILPGKT IGIIGGGQLG RMMALAAKEM GYKIAVLDPT KNSPCAQVAD
60 70 80 90 100
IEIVASYDDL KAIQHLAEIS DVVTYEFENI DYRCLQWLEK HAYLPQGSQL
110 120 130 140 150
LSKTQNRFTE KNAIEKAGLP VATYRLVQNQ EQLTEAIAEL SYPSVLKTTT
160 170 180 190 200
GGYDGKGQVV LRSEADVDEA RKLANAAECI LEKWVPFEKE VSVIVIRSVS
210 220 230 240 250
GETKVFPVAE NIHVNNILHE SIVPARITEE LSQKAIAYAK VLADELELVG
260 270 280 290 300
TLAVEMFATA DGEIYINELA PRPHNSGHYT QDACETSQFG QHIRAICNLP
310 320 330 340 350
LGETNLLKPV VMVNILGEHI EGVLRQVNRL TGCYLHLYGK EEAKAQRKMG
360 370 380
HVNILNDNIE VALEKAKSLH IWDHQEQLLE GKR
Length:383
Mass (Da):42,517
Last modified:June 1, 2003 - v1
Checksum:iBE0E5E1A56F88287
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP24325.1.
AE017334 Genomic DNA. Translation: AAT29376.2.
AE017225 Genomic DNA. Translation: AAT52607.1.
CP010813 Genomic DNA. Translation: AJG27238.1.
RefSeqiNP_842839.1. NC_003997.3.
WP_000196795.1. NZ_KN050651.1.
YP_016901.2. NC_007530.2.
YP_026556.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP24325; AAP24325; BA_0289.
AAT29376; AAT29376; GBAA_0289.
AAT52607; AAT52607; BAS0276.
AIF54758; AIF54758; HYU01_01585.
AIK06399; AIK06399; DH23_0273.
AIK33274; AIK33274; DJ48_3918.
AIK51583; AIK51583; DJ45_5598.
AIK60164; AIK60164; DJ44_4149.
AIM04256; AIM04256; BACvac02_0350.
AIM09641; AIM09641; BAHan_0355.
KFJ82806; KFJ82806; DJ42_4801.
KFL76897; KFL76897; DJ49_5205.
KFL79598; KFL79598; DJ43_4835.
GeneIDi1085688.
2851232.
KEGGiban:BA_0289.
bar:GBAA_0289.
bat:BAS0276.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP24325.1.
AE017334 Genomic DNA. Translation: AAT29376.2.
AE017225 Genomic DNA. Translation: AAT52607.1.
CP010813 Genomic DNA. Translation: AJG27238.1.
RefSeqiNP_842839.1. NC_003997.3.
WP_000196795.1. NZ_KN050651.1.
YP_016901.2. NC_007530.2.
YP_026556.1. NC_005945.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198094.BA_0289.

Protocols and materials databases

DNASUi1085688.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP24325; AAP24325; BA_0289.
AAT29376; AAT29376; GBAA_0289.
AAT52607; AAT52607; BAS0276.
AIF54758; AIF54758; HYU01_01585.
AIK06399; AIK06399; DH23_0273.
AIK33274; AIK33274; DJ48_3918.
AIK51583; AIK51583; DJ45_5598.
AIK60164; AIK60164; DJ44_4149.
AIM04256; AIM04256; BACvac02_0350.
AIM09641; AIM09641; BAHan_0355.
KFJ82806; KFJ82806; DJ42_4801.
KFL76897; KFL76897; DJ49_5205.
KFL79598; KFL79598; DJ43_4835.
GeneIDi1085688.
2851232.
KEGGiban:BA_0289.
bar:GBAA_0289.
bat:BAS0276.

Phylogenomic databases

HOGENOMiHOG000034029.
KOiK01589.
OMAiDSPCGQV.
OrthoDBiEOG6QZMX7.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00942.
BioCyciANTHRA:PURK-MONOMER.
BANT260799:GJAJ-318-MONOMER.
BANT261594:GJ7F-327-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01928. PurK.
InterProiIPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006140. D-isomer_DH_NAD-bd.
IPR016185. PreATP-grasp_dom.
IPR005875. PurK.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF02826. 2-Hacid_dh_C. 1 hit.
PF02222. ATP-grasp. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01161. purK. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
    Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
    , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
    Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AmesImported and Ames / isolate PortonImported.
  2. "Complete genome sequence of Bacillus anthracis Sterne."
    Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SterneImported.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames AncestorImported and Ames ancestorImported.
  4. Joardar V., Shrivastava S., Brinkac L.M., Harkins D.M., Durkin A.S., Sutton G.
    Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: AmesImported.
  5. "Genome sequence of Bacillus anthracis Pollino isolated from a bovine anthrax-burial site in Italy."
    Fasanella A., Braun P., Grass G., Hanczaruk M., Aceti A., Serrecchia L., Marino L., Georgi E., Antwerpen M.H.
    Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: PollinoImported.

Entry informationi

Entry nameiQ81ZH7_BACAN
AccessioniPrimary (citable) accession number: Q81ZH7
Secondary accession number(s): E9QVL4
, E9QVL5, Q6I4C4, Q6KY26
Entry historyi
Integrated into UniProtKB/TrEMBL: June 1, 2003
Last sequence update: June 1, 2003
Last modified: April 29, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.