Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

N5-carboxyaminoimidazole ribonucleotide synthase

Gene

purK

Organism
Bacillus anthracis
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO3- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).UniRule annotation
Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).UniRule annotation

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. N5-carboxyaminoimidazole ribonucleotide synthase (purK), N5-carboxyaminoimidazole ribonucleotide synthase (purK), N5-carboxyaminoimidazole ribonucleotide synthase (purK), N5-carboxyaminoimidazole ribonucleotide synthase (purK)
  2. N5-carboxyaminoimidazole ribonucleotide mutase (purE), N5-carboxyaminoimidazole ribonucleotide mutase (purE), N5-carboxyaminoimidazole ribonucleotide mutase (purE)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107ATPUniRule annotation1
Binding sitei147ATPUniRule annotation1
Binding sitei190ATPUniRule annotation1
Binding sitei213ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi152 – 158ATPUniRule annotation7
Nucleotide bindingi182 – 185ATPUniRule annotation4
Nucleotide bindingi267 – 268ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotation, LyaseImported
Biological processPurine biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00942.

Names & Taxonomyi

Protein namesi
Recommended name:
N5-carboxyaminoimidazole ribonucleotide synthaseUniRule annotation (EC:6.3.4.18UniRule annotation)
Short name:
N5-CAIR synthaseUniRule annotation
Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide synthetaseUniRule annotation
Gene namesi
Name:purKUniRule annotationImported
Ordered Locus Names:GBAA_0289Imported
ORF Names:BASH2_05529Imported, BF27_1667Imported, BVB96_01675Imported, BZG08_01680Imported, DH23_23440Imported
OrganismiBacillus anthracisImported
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000187792 Componenti: Chromosome
  • UP000000594 Componenti: Chromosome
  • UP000185489 Componenti: Chromosome
  • UP000186718 Componenti: Unassembled WGS sequence
  • UP000031920 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi198094.BA_0289.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini111 – 297ATP-graspInterPro annotationAdd BLAST187

Sequence similaritiesi

Belongs to the PurK/PurT family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CY8. Bacteria.
COG0026. LUCA.
HOGENOMiHOG000034029.
KOiK01589.
OMAiDSPCGQV.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
HAMAPiMF_01928. PurK. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006140. D-isomer_DH_NAD-bd.
IPR016185. PreATP-grasp_dom.
IPR005875. PurK.
IPR011054. Rudment_hybrid_motif.
PfamiView protein in Pfam
PF02826. 2-Hacid_dh_C. 1 hit.
PF02222. ATP-grasp. 1 hit.
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01161. purK. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q81ZH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRIILPGKT IGIIGGGQLG RMMALAAKEM GYKIAVLDPT KNSPCAQVAD
60 70 80 90 100
IEIVASYDDL KAIQHLAEIS DVVTYEFENI DYRCLQWLEK HAYLPQGSQL
110 120 130 140 150
LSKTQNRFTE KNAIEKAGLP VATYRLVQNQ EQLTEAIAEL SYPSVLKTTT
160 170 180 190 200
GGYDGKGQVV LRSEADVDEA RKLANAAECI LEKWVPFEKE VSVIVIRSVS
210 220 230 240 250
GETKVFPVAE NIHVNNILHE SIVPARITEE LSQKAIAYAK VLADELELVG
260 270 280 290 300
TLAVEMFATA DGEIYINELA PRPHNSGHYT QDACETSQFG QHIRAICNLP
310 320 330 340 350
LGETNLLKPV VMVNILGEHI EGVLRQVNRL TGCYLHLYGK EEAKAQRKMG
360 370 380
HVNILNDNIE VALEKAKSLH IWDHQEQLLE GKR
Length:383
Mass (Da):42,517
Last modified:June 1, 2003 - v1
Checksum:iBE0E5E1A56F88287
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017334 Genomic DNA. Translation: AAT29376.2.
CP009902 Genomic DNA. Translation: AJH86860.1.
CP018903 Genomic DNA. Translation: APT23932.1.
CP019588 Genomic DNA. Translation: AQM44372.1.
AP014833 Genomic DNA. Translation: BAR78936.1.
MJAW01000042 Genomic DNA. Translation: OKA48696.1.
RefSeqiWP_000196795.1. NZ_MLJX01000002.1.

Genome annotation databases

EnsemblBacteriaiAAT29376; AAT29376; GBAA_0289.
AJH86860; AJH86860; BF27_1667.
KOM62801; KOM62801; AB168_06515.
KOM67663; KOM67663; AB166_09945.
KOM70161; KOM70161; AB165_26240.
KOM77872; KOM77872; AB167_15040.
KOM81131; KOM81131; AB164_27110.
KOM92610; KOM92610; AB169_13265.
KON03265; KON03265; AB170_23235.
KON16492; KON16492; AB171_19460.
KON25587; KON25587; AB163_03480.
KOR61507; KOR61507; ADT21_09145.
KOR65703; KOR65703; ADT20_21210.
KEGGibanh:HYU01_01585.
bar:GBAA_0289.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017334 Genomic DNA. Translation: AAT29376.2.
CP009902 Genomic DNA. Translation: AJH86860.1.
CP018903 Genomic DNA. Translation: APT23932.1.
CP019588 Genomic DNA. Translation: AQM44372.1.
AP014833 Genomic DNA. Translation: BAR78936.1.
MJAW01000042 Genomic DNA. Translation: OKA48696.1.
RefSeqiWP_000196795.1. NZ_MLJX01000002.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198094.BA_0289.

Protocols and materials databases

DNASUi1085688.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT29376; AAT29376; GBAA_0289.
AJH86860; AJH86860; BF27_1667.
KOM62801; KOM62801; AB168_06515.
KOM67663; KOM67663; AB166_09945.
KOM70161; KOM70161; AB165_26240.
KOM77872; KOM77872; AB167_15040.
KOM81131; KOM81131; AB164_27110.
KOM92610; KOM92610; AB169_13265.
KON03265; KON03265; AB170_23235.
KON16492; KON16492; AB171_19460.
KON25587; KON25587; AB163_03480.
KOR61507; KOR61507; ADT21_09145.
KOR65703; KOR65703; ADT20_21210.
KEGGibanh:HYU01_01585.
bar:GBAA_0289.

Phylogenomic databases

eggNOGiENOG4105CY8. Bacteria.
COG0026. LUCA.
HOGENOMiHOG000034029.
KOiK01589.
OMAiDSPCGQV.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00942.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
HAMAPiMF_01928. PurK. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006140. D-isomer_DH_NAD-bd.
IPR016185. PreATP-grasp_dom.
IPR005875. PurK.
IPR011054. Rudment_hybrid_motif.
PfamiView protein in Pfam
PF02826. 2-Hacid_dh_C. 1 hit.
PF02222. ATP-grasp. 1 hit.
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01161. purK. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQ81ZH7_BACAN
AccessioniPrimary (citable) accession number: Q81ZH7
Secondary accession number(s): E9QVL4
, E9QVL5, Q6I4C4, Q6KY26
Entry historyiIntegrated into UniProtKB/TrEMBL: June 1, 2003
Last sequence update: June 1, 2003
Last modified: April 12, 2017
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.