Q81ZH0 (PUR5_BACAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	BA_0296, GBAA_0296, BAS0283

Organism

Bacillus anthracis

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741_B
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00741_B.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 346

346

Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741_B

PRO_0000148194

Secondary structure

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346

Helix Strand Turn

Details...

Turn

19 – 26

8

Helix

27 – 29

3

Beta strand

44 – 46

3

Beta strand

53 – 63

11

Helix

68 – 74

7

Helix

80 – 93

14

Turn

94 – 96

3

Beta strand

98 – 110

13

Helix

113 – 130

18

Beta strand

133 – 141

9

Beta strand

152 – 162

11

Beta strand

177 – 182

6

Beta strand

184 – 186

3

Helix

191 – 199

9

Beta strand

211 – 213

3

Helix

217 – 221

5

Helix

229 – 238

10

Helix

251 – 255

5

Helix

257 – 259

3

Beta strand

264 – 269

6

Helix

277 – 286

10

Helix

290 – 293

4

Turn

294 – 296

3

Beta strand

301 – 307

7

Turn

309 – 311

3

Helix

312 – 322

11

Beta strand

326 – 341

16

Sequences

	Sequence		Length	Mass (Da)
	Q81ZH0 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 6A4825D9FF515A43	FASTA	346	37,256
	10 20 30 40 50 60 MANAYKQAGV DIEAGYEAVS RMKKHVQTTM RKEVLGGLGG FGGMFDLSKF ALEEPVLVSG 70 80 90 100 110 120 TDGVGTKLML AFMADKHDTI GIDAVAMCVN DIVVQGAEPL FFLDYIACGK AEPSKIENIV 130 140 150 160 170 180 KGISEGCRQA GCALIGGETA EMPGMYSTEE YDLAGFTVGI VDKKKIVTGE KIEAGHVLIG 190 200 210 220 230 240 LASSGIHSNG YSLVRKVLLE DGELSLDRIY GRLELPLGEE LLKPTKIYVK PILELLKNHE 250 260 270 280 290 300 VYGMAHITGG GFIENIPRML PEGIGAEIEL GSWKIQPIFS LLQEVGKLEE KEMFNIFNMG 310 320 330 340 IGMVVAVKEE DAKDIVRLLE EQGETARIIG RTVQGAGVTF NGGKAL

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE016879 Genomic DNA. Translation: AAP24332.1.
AE017334 Genomic DNA. Translation: AAT29383.1.
AE017225 Genomic DNA. Translation: AAT52614.1.

RefSeq

NP_842846.1. NC_003997.3.
YP_016908.1. NC_007530.2.
YP_026563.1. NC_005945.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BTU	X-ray	2.31	A/B	1-346	[»]

ProteinModelPortal

Q81ZH0.

SMR

Q81ZH0. Positions 13-341.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBBACT00000012294; EBBACP00000012050; EBBACG00000012286.
EBBACT00000014333; EBBACP00000013954; EBBACG00000014325.
EBBACT00000019389; EBBACP00000018874; EBBACG00000019380.

GeneID

1085673.
2816181.
2851164.

GenomeReviews

Gene locus BA_0296 in contig AE016879_GR.
Gene locus BAS0283 in contig AE017225_GR.
Gene locus GBAA_0296 in contig AE017334_GR.

KEGG

ban:BA_0296.
bar:GBAA_0296.
bat:BAS0283.

TIGR

BA_0296.
GBAA_0296.

Phylogenomic databases

GeneTree

EBGT00050000002660.

HOGENOM

HBG531222.

OMA

GIDMIAM.

ProtClustDB

PRK05385.

Enzyme and pathway databases

BioCyc

BANT260799:BAS0283-MONOMER.
BANT261594:GBAA0296-MONOMER.

Family and domain databases

HAMAP

MF_00741_B. AIRS_B.
[Tree]

InterPro

IPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
[Graphical view]

KO

K01933.

Pfam

PF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]

SUPFAM

SSF56042. AIR_synth_C. 1 hit.
SSF55326. PurM_N-like. 1 hit.

TIGRFAMs

TIGR00878. PurM. 1 hit.

ProtoNet

Search...

Entry information

Entry name

PUR5_BACAN

Accession

Primary (citable) accession number: Q81ZH0
Secondary accession number(s): Q6I4B7, Q6KY19

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	June 1, 2003
Last modified:	November 16, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program