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Q81Z97

- TOP3_BACAN

UniProt

Q81Z97 - TOP3_BACAN

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Protein
DNA topoisomerase 3
Gene
topB, BA_0375, GBAA_0375, BAS0361
Organism
Bacillus anthracis
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Magnesium; catalytic By similarity
Sitei61 – 611Interaction with DNA By similarity
Metal bindingi105 – 1051Magnesium; catalytic By similarity
Sitei168 – 1681Interaction with DNA By similarity
Sitei176 – 1761Interaction with DNA By similarity
Active sitei310 – 3101O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei312 – 3121Interaction with DNA By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBANT260799:GJAJ-403-MONOMER.
BANT261594:GJ7F-413-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase III
Gene namesi
Name:topB
Ordered Locus Names:BA_0375, GBAA_0375, BAS0361
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000000427: Chromosome, UP000000594: Chromosome, UP000005639: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 729729DNA topoisomerase 3UniRule annotation
PRO_0000286359Add
BLAST

Interactioni

Protein-protein interaction databases

IntActiQ81Z97. 2 interactions.
STRINGi198094.BA_0375.

Structurei

3D structure databases

ProteinModelPortaliQ81Z97.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 136134Toprim
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni187 – 1926Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Phylogenomic databases

eggNOGiCOG0551.
HOGENOMiHOG000086847.
KOiK03169.
OMAiSIAVGPY.
OrthoDBiEOG60CWM6.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00953. Topoisom_3_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01056. topB. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81Z97-1 [UniParc]FASTAAdd to Basket

« Hide

MAKSVVIAEK PSVARDIARV LKCDKKGNGY LEGSKYIVTW ALGHLVTLAD    50
PESYDVKYKK WNLEDLPMLP ERLKLTVIKQ TGKQFNAVKS QLLRKDVNEI 100
IVATDAGREG ELVARWIIDK VRINKPIKRL WISSVTDKAI KDGFANLKPG 150
KAYDNLYASA VARSEADWYI GLNATRALTT RFNAQLNCGR VQTPTVAMIA 200
NREDEIKNFK AQTYYGIEAQ TTNQLKLTWQ DANGNSRSFN KEKIDGIVKG 250
LDKHNATVLE IDKKQKKSFS PGLYDLTELQ RDANKKFGYS AKETLNIMQK 300
LYEQHKVLTY PRTDSRYISS DIVGTLPERL KACGVGEYRP LAHKVLQKPI 350
KANKSFVDDS KVSDHHAIIP TEGYVNFSAF TDKERKIYDL VVKRFLAVLF 400
PAFEYEQLTL RTKVGNETFI ARGKTILHAG WKEVYENRFE DDDVTDDVKE 450
QLLPRIEKGD TLTVKLIMQT SGQTKAPARF NEATLLSAME NPTKYMDTQN 500
KQLADTLKST GGLGTVATRA DIIDKLFNSF LIEKRGKDIH ITSKGRQLLD 550
LVPEELKSPT LTGEWEQKLE AIAKGKLKKE VFISEMKNYT KEIVSEIKSS 600
DKKYKHDNIS TKSCPDCGKP MLEVNGKKGK MLVCQDRECG HRKNVSRTTN 650
ARCPQCKKKL ELRGEGAGQI FACKCGYREK LSTFQERRKK ESGNKADKRD 700
VQKYMKQQKK EEEPLNNPFA EALKKLKFD 729
Length:729
Mass (Da):82,812
Last modified:June 1, 2003 - v1
Checksum:i8DEB7E647EC3CB0F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016879 Genomic DNA. Translation: AAP24405.1.
AE017334 Genomic DNA. Translation: AAT29470.1.
AE017225 Genomic DNA. Translation: AAT52692.1.
RefSeqiNP_842919.1. NC_003997.3.
YP_016995.1. NC_007530.2.
YP_026641.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP24405; AAP24405; BA_0375.
AAT29470; AAT29470; GBAA_0375.
AAT52692; AAT52692; BAS0361.
GeneIDi1084121.
2819802.
2849354.
KEGGiban:BA_0375.
bar:GBAA_0375.
bat:BAS0361.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016879 Genomic DNA. Translation: AAP24405.1 .
AE017334 Genomic DNA. Translation: AAT29470.1 .
AE017225 Genomic DNA. Translation: AAT52692.1 .
RefSeqi NP_842919.1. NC_003997.3.
YP_016995.1. NC_007530.2.
YP_026641.1. NC_005945.1.

3D structure databases

ProteinModelPortali Q81Z97.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q81Z97. 2 interactions.
STRINGi 198094.BA_0375.

Protocols and materials databases

DNASUi 1084121.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP24405 ; AAP24405 ; BA_0375 .
AAT29470 ; AAT29470 ; GBAA_0375 .
AAT52692 ; AAT52692 ; BAS0361 .
GeneIDi 1084121.
2819802.
2849354.
KEGGi ban:BA_0375.
bar:GBAA_0375.
bat:BAS0361.

Phylogenomic databases

eggNOGi COG0551.
HOGENOMi HOG000086847.
KOi K03169.
OMAi SIAVGPY.
OrthoDBi EOG60CWM6.

Enzyme and pathway databases

BioCyci BANT260799:GJAJ-403-MONOMER.
BANT261594:GJ7F-413-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00953. Topoisom_3_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01056. topB. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
    Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
    , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
    Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames / isolate Porton.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames ancestor.
  3. "Complete genome sequence of Bacillus anthracis Sterne."
    Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sterne.

Entry informationi

Entry nameiTOP3_BACAN
AccessioniPrimary (citable) accession number: Q81Z97
Secondary accession number(s): Q6I439, Q6KXU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: June 1, 2003
Last modified: September 3, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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