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Protein

DNA topoisomerase 3

Gene

topB

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9Magnesium; catalyticUniRule annotation1
Metal bindingi105Magnesium; catalyticUniRule annotation1
Active sitei310O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IIIUniRule annotation
Gene namesi
Name:topBUniRule annotation
Ordered Locus Names:BA_0375, GBAA_0375, BAS0361
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome
  • UP000000427 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002863591 – 729DNA topoisomerase 3Add BLAST729

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei61Interaction with DNAUniRule annotation1
Sitei168Interaction with DNAUniRule annotation1
Sitei176Interaction with DNAUniRule annotation1
Sitei312Interaction with DNAUniRule annotation1

Protein-protein interaction databases

IntActiQ81Z97, 2 interactors
STRINGi260799.BAS0361

Structurei

3D structure databases

ProteinModelPortaliQ81Z97
SMRiQ81Z97
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 136ToprimUniRule annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni187 – 192Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9U Bacteria
COG0550 LUCA
COG0551 LUCA
HOGENOMiHOG000086847
KOiK03169
OMAiTYPRVDT

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03362 TOPRIM_TopoIA_TopoIII, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 2 hits
2.70.20.10, 2 hits
HAMAPiMF_00953 Topoisom_3_prok, 1 hit
InterProiView protein in InterPro
IPR000380 Topo_IA
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR005738 TopoIII
IPR006171 TOPRIM_domain
IPR034144 TOPRIM_TopoIII
PANTHERiPTHR11390 PTHR11390, 1 hit
PfamiView protein in Pfam
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 1 hit
TIGRFAMsiTIGR01056 topB, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

Q81Z97-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKSVVIAEK PSVARDIARV LKCDKKGNGY LEGSKYIVTW ALGHLVTLAD
60 70 80 90 100
PESYDVKYKK WNLEDLPMLP ERLKLTVIKQ TGKQFNAVKS QLLRKDVNEI
110 120 130 140 150
IVATDAGREG ELVARWIIDK VRINKPIKRL WISSVTDKAI KDGFANLKPG
160 170 180 190 200
KAYDNLYASA VARSEADWYI GLNATRALTT RFNAQLNCGR VQTPTVAMIA
210 220 230 240 250
NREDEIKNFK AQTYYGIEAQ TTNQLKLTWQ DANGNSRSFN KEKIDGIVKG
260 270 280 290 300
LDKHNATVLE IDKKQKKSFS PGLYDLTELQ RDANKKFGYS AKETLNIMQK
310 320 330 340 350
LYEQHKVLTY PRTDSRYISS DIVGTLPERL KACGVGEYRP LAHKVLQKPI
360 370 380 390 400
KANKSFVDDS KVSDHHAIIP TEGYVNFSAF TDKERKIYDL VVKRFLAVLF
410 420 430 440 450
PAFEYEQLTL RTKVGNETFI ARGKTILHAG WKEVYENRFE DDDVTDDVKE
460 470 480 490 500
QLLPRIEKGD TLTVKLIMQT SGQTKAPARF NEATLLSAME NPTKYMDTQN
510 520 530 540 550
KQLADTLKST GGLGTVATRA DIIDKLFNSF LIEKRGKDIH ITSKGRQLLD
560 570 580 590 600
LVPEELKSPT LTGEWEQKLE AIAKGKLKKE VFISEMKNYT KEIVSEIKSS
610 620 630 640 650
DKKYKHDNIS TKSCPDCGKP MLEVNGKKGK MLVCQDRECG HRKNVSRTTN
660 670 680 690 700
ARCPQCKKKL ELRGEGAGQI FACKCGYREK LSTFQERRKK ESGNKADKRD
710 720
VQKYMKQQKK EEEPLNNPFA EALKKLKFD
Length:729
Mass (Da):82,812
Last modified:June 1, 2003 - v1
Checksum:i8DEB7E647EC3CB0F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA Translation: AAP24405.1
AE017334 Genomic DNA Translation: AAT29470.1
AE017225 Genomic DNA Translation: AAT52692.1
RefSeqiNP_842919.1, NC_003997.3
WP_001140209.1, NZ_NRJB01000008.1
YP_026641.1, NC_005945.1

Genome annotation databases

EnsemblBacteriaiAAP24405; AAP24405; BA_0375
AAT29470; AAT29470; GBAA_0375
AAT52692; AAT52692; BAS0361
GeneIDi1084121
2849354
KEGGiban:BA_0375
bar:GBAA_0375
bat:BAS0361
PATRICifig|198094.11.peg.370

Similar proteinsi

Entry informationi

Entry nameiTOP3_BACAN
AccessioniPrimary (citable) accession number: Q81Z97
Secondary accession number(s): Q6I439, Q6KXU9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: June 1, 2003
Last modified: April 25, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health