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Q81Z97 (TOP3_BACAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 3

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase III
Gene names
Name:topB
Ordered Locus Names:BA_0375, GBAA_0375, BAS0361
OrganismBacillus anthracis [Reference proteome] [HAMAP]
Taxonomic identifier1392 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Protein attributes

Sequence length729 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00953

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00953

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00953

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 729729DNA topoisomerase 3 HAMAP-Rule MF_00953
PRO_0000286359

Regions

Domain3 – 136134Toprim
Region187 – 1926Interaction with DNA By similarity

Sites

Active site3101O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding91Magnesium; catalytic By similarity
Metal binding1051Magnesium; catalytic By similarity
Site611Interaction with DNA By similarity
Site1681Interaction with DNA By similarity
Site1761Interaction with DNA By similarity
Site3121Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q81Z97 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 8DEB7E647EC3CB0F

FASTA72982,812
        10         20         30         40         50         60 
MAKSVVIAEK PSVARDIARV LKCDKKGNGY LEGSKYIVTW ALGHLVTLAD PESYDVKYKK 

        70         80         90        100        110        120 
WNLEDLPMLP ERLKLTVIKQ TGKQFNAVKS QLLRKDVNEI IVATDAGREG ELVARWIIDK 

       130        140        150        160        170        180 
VRINKPIKRL WISSVTDKAI KDGFANLKPG KAYDNLYASA VARSEADWYI GLNATRALTT 

       190        200        210        220        230        240 
RFNAQLNCGR VQTPTVAMIA NREDEIKNFK AQTYYGIEAQ TTNQLKLTWQ DANGNSRSFN 

       250        260        270        280        290        300 
KEKIDGIVKG LDKHNATVLE IDKKQKKSFS PGLYDLTELQ RDANKKFGYS AKETLNIMQK 

       310        320        330        340        350        360 
LYEQHKVLTY PRTDSRYISS DIVGTLPERL KACGVGEYRP LAHKVLQKPI KANKSFVDDS 

       370        380        390        400        410        420 
KVSDHHAIIP TEGYVNFSAF TDKERKIYDL VVKRFLAVLF PAFEYEQLTL RTKVGNETFI 

       430        440        450        460        470        480 
ARGKTILHAG WKEVYENRFE DDDVTDDVKE QLLPRIEKGD TLTVKLIMQT SGQTKAPARF 

       490        500        510        520        530        540 
NEATLLSAME NPTKYMDTQN KQLADTLKST GGLGTVATRA DIIDKLFNSF LIEKRGKDIH 

       550        560        570        580        590        600 
ITSKGRQLLD LVPEELKSPT LTGEWEQKLE AIAKGKLKKE VFISEMKNYT KEIVSEIKSS 

       610        620        630        640        650        660 
DKKYKHDNIS TKSCPDCGKP MLEVNGKKGK MLVCQDRECG HRKNVSRTTN ARCPQCKKKL 

       670        680        690        700        710        720 
ELRGEGAGQI FACKCGYREK LSTFQERRKK ESGNKADKRD VQKYMKQQKK EEEPLNNPFA 


EALKKLKFD 

« Hide

References

[1]"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. expand/collapse author list , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Ames / isolate Porton.
[2]"The complete genome sequence of Bacillus anthracis Ames 'Ancestor'."
Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D., Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.
J. Bacteriol. 191:445-446(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Ames ancestor.
[3]"Complete genome sequence of Bacillus anthracis Sterne."
Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sterne.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016879 Genomic DNA. Translation: AAP24405.1.
AE017334 Genomic DNA. Translation: AAT29470.1.
AE017225 Genomic DNA. Translation: AAT52692.1.
RefSeqNP_842919.1. NC_003997.3.
YP_016995.1. NC_007530.2.
YP_026641.1. NC_005945.1.

3D structure databases

ProteinModelPortalQ81Z97.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ81Z97. 2 interactions.
STRING198094.BA_0375.

Protocols and materials databases

DNASU1084121.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP24405; AAP24405; BA_0375.
AAT29470; AAT29470; GBAA_0375.
AAT52692; AAT52692; BAS0361.
GeneID1084121.
2819802.
2849354.
KEGGban:BA_0375.
bar:GBAA_0375.
bat:BAS0361.

Phylogenomic databases

eggNOGCOG0551.
HOGENOMHOG000086847.
KOK03169.
OMASIAVGPY.
OrthoDBEOG60CWM6.

Enzyme and pathway databases

BioCycBANT260799:GJAJ-403-MONOMER.
BANT261594:GJ7F-413-MONOMER.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00953. Topoisom_3_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01056. topB. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP3_BACAN
AccessionPrimary (citable) accession number: Q81Z97
Secondary accession number(s): Q6I439, Q6KXU9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families