Q81YY0 (GLSA1_BACAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutaminase 1
EC=3.5.1.2

Gene names

Name:	glsA1
Synonyms:	glsA-1
Ordered Locus Names:	BA_0499, GBAA_0499, BAS0471

Organism

Bacillus anthracis [Reference proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-glutamine + H2O = L-glutamate + NH3. HAMAP-Rule MF_00313
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the glutaminase family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	glutaminase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Glutaminase 1 HAMAP-Rule MF_00313		PRO_0000110588
Sites
	Binding site	65	1	Substrate By similarity
	Binding site	117	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	169	1	Substrate By similarity
	Binding site	193	1	Substrate By similarity
	Binding site	245	1	Substrate By similarity
	Binding site	263	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81YY0 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 56FFE294D77F0B35	FASTA	309	34,048
	10 20 30 40 50 60 MQCIETNNLQ QLLEQVKPYT KKGKLATYIP ELGNANPDDL GIAIFHKETE YIHAGNSQTL 70 80 90 100 110 120 FTLQSISKVI TLALALLDRG EEYVFSKVGM EPTGDPFNSI IKLETTSPSK PLNPMINAGA 130 140 150 160 170 180 LAITSMLAGK DNEEKMERIL HFVREITDNP TINYSSKVAN SELETAYLNR SLCYYMKQNG 190 200 210 220 230 240 IIDCDIEELM DLYTRQCAVE VNCIDLARIG LIFAMDGYDP YKKKQIIPKH ITKICKTFMV 250 260 270 280 290 300 TCGMYNESGE FAIRVGIPAK SGVAGGIFGC VKGEMGIGIF GPALDANGNS IAGFKILELL SAQEGWSIF

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"The complete genome sequence of Bacillus anthracis Ames 'Ancestor'." Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D., Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A. J. Bacteriol. 191:445-446(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP24522.1. AE017334 Genomic DNA. Translation: AAT29592.1. AE017225 Genomic DNA. Translation: AAT52802.1.
RefSeq	NP_843036.1. NC_003997.3. YP_017117.1. NC_007530.2. YP_026751.1. NC_005945.1.
3D structure databases
ProteinModelPortal	Q81YY0.
SMR	Q81YY0. Positions 5-309.
ModBase	Search...
Protein-protein interaction databases
STRING	198094.BA_0499.
Protocols and materials databases
DNASU	1086143.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAP24522; AAP24522; BA_0499. AAT29592; AAT29592; GBAA_0499. AAT52802; AAT52802; BAS0471.
GeneID	1086143. 2816157. 2848080.
KEGG	ban:BA_0499. bar:GBAA_0499. bat:BAS0471.
Phylogenomic databases
eggNOG	COG2066.
HOGENOM	HOG000216890.
KO	K01425.
OMA	DRGEDEV.
ProtClustDB	PRK00971.
Enzyme and pathway databases
BioCyc	BANT260799:GJAJ-514-MONOMER. BANT261594:GJ7F-536-MONOMER.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
HAMAP	MF_00313. Glutaminase.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR015868. Glutaminase. [Graphical view]
PANTHER	PTHR12544. PTHR12544. 1 hit.
Pfam	PF04960. Glutaminase. 1 hit. [Graphical view]
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
TIGRFAMs	TIGR03814. Gln_ase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLSA1_BACAN

Accession

Primary (citable) accession number: Q81YY0
Secondary accession number(s): Q6I3S9, Q6KXJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program