Reviewed, UniProtKB/Swiss-Prot Q81YW9 (UGTP_BACAN)

Names and origin

Protein names

Recommended name:
    Processive diacylglycerol glucosyltransferase
    EC=2.4.1.-
Alternative name(s):
    UDP glucosyltransferase
    UDP-glucose:1,2-diacylglycerol-3-beta-D-glucosyltransferase
    Monoglucosyldiacylglycerol synthase
      Short name=MGlcDAG synthase
    Diglucosyldiacylglycerol synthase
    Beta-gentiobiosyldiacylglycerol synthase
    DGlcDAG synthase
    Glc2-DAG synthase
    Triglucosyldiacylglycerol synthase
      Short name=TGlcDAG synthase

Gene names

Name:	ugtP
Ordered Locus Names:	BA_0511, GBAA_0511, BAS0483

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is a processive glucosyltransferase involved in the synthesis of both bilayer- and non-bilayer-forming membrane glycolipids. Is able to successively transfer up to three glucosyl residues to diacylglycerol (DAG), thereby catalyzing the formation of mono-, di- and triglucosyldiacylglycerol. Diglucosyldiacylglycerol (Glc2-DAG) is the predominant glycolipid found in Bacillales and is also used as a membrane anchor for lipoteichoic acid (LTA) By similarity.
Catalytic activity	UDP-glucose + 1,2-diacylglycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl)-sn-glycerol. HAMAP MF_01280 UDP-glucose + 1,2-diacyl-3-(O-beta-D-glucopyranosyl)-sn-glycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol. HAMAP MF_01280 UDP-glucose + 1,2-diacyl-3-(O-beta-D-glucopyranosyl(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl(1->6)-O-beta-D-glucopyranosyl(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol.
Pathway	Glycolipid metabolism; diglucosyl-diacylglycerol biosynthesis. HAMAP MF_01280
Sequence similarities	Belongs to the glycosyltransferase 1 family. UgtP subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW glycolipid biosynthetic process Inferred from electronic annotation. Source: HAMAP lipid glycosylation Inferred from electronic annotation. Source: InterPro
Molecular function	UDP-glucosyltransferase activity Inferred from electronic annotation. Source: HAMAP carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Processive diacylglycerol glucosyltransferase HAMAP MF_01280		PRO_0000308447

Sequences

	Sequence		Length	Mass (Da)
	Q81YW9-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 258505FF5A850662	FASTA	388	43,761
	10 20 30 40 50 60 MIKNPKVLIL TAHYGNGHVQ VAKTLEQTFR QKGIKDVIVC DLFGESHPVI TDITKYLYLK 70 80 90 100 110 120 SYTIGKELYR LFYYGVEKIY DKKIASWYAN FGRKRLKLLL QAEKPDIVIN TFPIIAVPEL 130 140 150 160 170 180 KKQTGISIPV YNVLTDFCVH KIWIHREVDR YFVATDHVKK VMVDIGVPAE QIVETGIPIR 190 200 210 220 230 240 SSFELKINPD IIYNKYQLCK NKKILLIVAG AHGVLGSVKE LCQSFMSVPD LQVVVVCGKN 250 260 270 280 290 300 EALKQDLVGV QETNPDALKV FGYVENIDEL FRVTSCMITK PGGITLSEAA ALQVPVILYK 310 320 330 340 350 360 PVPGQENENA MYFERKGAAV VIRDDSEVFA KTEALLQDDM KLLQMKEAMK SIYRPEPADH 370 380 IVDTILAENH VEPNHIPIKS PALAQSFT

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.
[3]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016879 Genomic DNA. Translation: AAP24533.1. AE017334 Genomic DNA. Translation: AAT29605.1. AE017225 Genomic DNA. Translation: AAT52814.1.
RefSeq	NP_843047.1. YP_017130.1. YP_026763.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT28. Glycosyltransferase Family 28.
Genome annotation databases
GeneID	1087727. 2817605. 2850475.
GenomeReviews	Gene locus BA_0511 in contig AE016879_GR. Gene locus BAS0483 in contig AE017225_GR. Gene locus GBAA0511 in contig AE017334_GR.
KEGG	ban:BA0511. bar:GBAA0511. bat:BAS0483.
TIGR	BA_0511. GBAA_0511.
Phylogenomic databases
HOGENOM	Q81YW9.
OMA	Q81YW9. PDVIICT.
Enzyme and pathway databases
BioCyc	BANT260799:BAS0483-MON. BANT261594:GBAA0511-MON.
Family and domain databases
HAMAP	MF_01280. [Tree]
InterPro	IPR007235. Glyco_trans_28_C. IPR009695. MGDG_synth. [Graphical view]
Pfam	PF04101. Glyco_tran_28_C. 1 hit. PF06925. MGDG_synth. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UGTP_BACAN

Accession

Primary (citable) accession number: Q81YW9
Secondary accession number(s): Q6I3R7, Q6KXI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)