amyS

Functionⁱ

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	132	Calcium 1UniRule annotation	1
Metal bindingⁱ	189	Calcium 2UniRule annotation	1
Metal bindingⁱ	211	Calcium 2; via carbonyl oxygenUniRule annotation	1
Metal bindingⁱ	213	Calcium 2UniRule annotation	1
Metal bindingⁱ	224	Calcium 1UniRule annotation	1
Metal bindingⁱ	230	Calcium 1UniRule annotation	1
Metal bindingⁱ	232	Calcium 2UniRule annotation	1
Active siteⁱ	261	NucleophileUniRule annotation	1
Metal bindingⁱ	265	Calcium 1; via carbonyl oxygenUniRule annotation	1
Active siteⁱ	291	Proton donorUniRule annotation	1
Metal bindingⁱ	330	Calcium 3; via carbonyl oxygenUniRule annotation	1
Metal bindingⁱ	437	Calcium 3UniRule annotation	1
Metal bindingⁱ	460	Calcium 3UniRule annotation	1

GO - Molecular functionⁱ

alpha-amylase activity
Source: TIGR
Inferred from sequence or structural similarityⁱ
- 12721629
alpha-amylase activity (releasing maltohexaose) Source: UniProtKB-EC
calcium ion binding Source: InterPro

Complete GO annotation...

GO - Biological processⁱ

carbohydrate metabolic process
Source: TIGR
Inferred from sequence or structural similarityⁱ
- 12721629

Complete GO annotation...

Keywordsⁱ

Molecular function	GlycosidaseImported, Hydrolase
Ligand	CalciumUniRule annotation, Metal-bindingUniRule annotation

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GH13. Glycoside Hydrolase Family 13.

CAZyⁱ

GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyⁱ

Protein namesⁱ	Submitted name: Alpha-amylaseImported (EC:3.2.1.1Imported)
Gene namesⁱ	Name:amySImported Ordered Locus Names:GBAA_3551Imported ORF Names:BASH2_02375Imported
Organismⁱ	Bacillus anthracisImported
Taxonomic identifierⁱ	1392 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Terrabacteria group › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group
Proteomesⁱ	UP000000594 Componentⁱ: Chromosome

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 27	Sequence analysisAdd BLAST		27
ChainⁱPRO_5010976184	28 – 513	Sequence analysisAdd BLAST		486

Interactionⁱ

Protein-protein interaction databases

IntActⁱ	Q81YJ4. 1 interactor.
STRINGⁱ	260799.BAS3291.

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	33 – 421	AamyInterPro annotationAdd BLAST		389

Keywords - Domainⁱ

SignalSequence analysis

Phylogenomic databases

eggNOGⁱ	ENOG4105E5K. Bacteria. COG0366. LUCA.
HOGENOMⁱ	HOG000094847.
KEGG Orthology (KO) More...KOⁱ	K01176.

Family and domain databases

InterProⁱ	View protein in InterPro IPR013776. A-amylase_thermo. IPR015237. Alpha-amylase_C_pro. IPR006047. Glyco_hydro_13_cat_dom. IPR017853. Glycoside_hydrolase_SF.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00128. Alpha-amylase. 1 hit. PF09154. DUF1939. 1 hit.
PIRSFⁱ	PIRSF001021. Alph-amls_thrmst. 1 hit.
SMARTⁱ	View protein in SMART SM00642. Aamy. 1 hit.
SUPFAMⁱ	SSF51445. SSF51445. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q81YJ4-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MFKKITIVGL SVVLFLPSIY GESKVYADTI NNGTLMQYFE WYAPSDRNHW 
        60         70         80         90        100
NRLRTDAENL AQKGITSVWI PPAYKGTTQN DVGYGAYDLY DLGEFNQKGT 
       110        120        130        140        150
VRTKYGTKAQ LKSAIEALHK QNIDVYGDVV MNHKGGADYT ETVTAVEVDR 
       160        170        180        190        200
NNRNVEVSGD YEISAWTGFN FPGRGDNYSN FKWKWYHFDG TDWDEGRKLN 
       210        220        230        240        250
RIYKFRGIGK AWDWEVSSEN GNYDYLMYAD LDFDHPDVAN EMKNWGTWYA 
       260        270        280        290        300
NELNLDGFRL DAVKHIDHEY LRDWVNHVRQ QTGKEMFTVA EYWQNDIQTL 
       310        320        330        340        350
NNYLAKVNYN QSVFDAPLHY NFHYASKGNG NYDMRNILNG TVMQNHPALA 
       360        370        380        390        400
VTLVENHDSQ PGQSLESVVS PWFKPLAYAF ILTRAEGYPS VFYGDYYGTS 
       410        420        430        440        450
GNSSYEIPAL KDKIDPILTA RKNFAYGTQR DYLDHPDVIG WTREGDSVHA 
       460        470        480        490        500
NSGLATLISD GPGGSKWMDV GKNNAGEVWH DMTGNQTNTV TINKDGWGQF 
       510 
HVSGGSVSIY VQQ

Length:513

Mass (Da):58,446

Last modified:June 1, 2003 - v1

Checksum:ⁱ558D6EF282FD159B

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE017334 Genomic DNA. Translation: AAT32659.1. AP014833 Genomic DNA. Translation: BAR75782.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_000473536.1. NZ_MVKJ01000003.1.

Genome annotation databases

EnsemblBacteriaⁱ	AAT32659; AAT32659; GBAA_3551. ANH87541; ANH87541; A8C77_17260.
KEGGⁱ	bar:GBAA_3551.
PATRICⁱ	fig\|1392.232.peg.2428.

Similar proteinsⁱ

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q81YJ4	A0A1V4BH77 UPI00098F173E	Bacillus anthracis	513	UniRef100_Q81YJ4	Cluster: Alpha-amylase	3

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q81YJ4	A0A0T8MDE0 Q9AQ54 C2N3V3 UPI00030877E0 C3E683 J8LHJ4 A0A0D1NHW9 C2WQG9 R8E3J2 A0A1B1SW15 +301	Streptococcus pneumoniae Bacillus megaterium Bacillus cereus ATCC 10876 Bacillus cereus Bacillus thuringiensis serovar pakistani str. T13001 Bacillus cereus VD154 Bacillus thuringiensis Sbt003 Bacillus cereus Rock4-2 Bacillus cereus VD133 Bacillus thuringiensis And more	513	UniRef90_A0A0T8MDE0	Cluster: Alpha-amylase	312

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q81YJ4	P06279 Q6LDN2 P70957 A0A063YP90 Q0MS47 UPI0001D589AA A0A150MM56 A0A178TZN1 UPI0000111C7C A0A0D8BWB1 +411	Geobacillus stearothermophilus (Bacillus stearothermophilus) Geobacillus sp. CAMR5420 Geobacillus sp. (strain C56-T3) Geobacillus kaustophilus Geobacillus sp. MAS1 Geobacillus sp. (strain Y412MC52) Geobacillus sp. SR74 Geobacillus stearothermophilus 10 Geobacillus thermoleovorans (Bacillus thermoleovorans) Geobacillus sp. A8 And more	549	UniRef50_P06279	Cluster: Alpha-amylase	422

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE017334 Genomic DNA. Translation: AAT32659.1. AP014833 Genomic DNA. Translation: BAR75782.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_000473536.1. NZ_MVKJ01000003.1.

3D structure databases

ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	Q81YJ4. 1 interactor.
STRINGⁱ	260799.BAS3291.

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GH13. Glycoside Hydrolase Family 13.

CAZyⁱ

GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	1084526.
Structural Biology Knowledgebase	Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAT32659; AAT32659; GBAA_3551. ANH87541; ANH87541; A8C77_17260.
KEGGⁱ	bar:GBAA_3551.
PATRICⁱ	fig\|1392.232.peg.2428.

Phylogenomic databases

eggNOGⁱ	ENOG4105E5K. Bacteria. COG0366. LUCA.
HOGENOMⁱ	HOG000094847.
KEGG Orthology (KO) More...KOⁱ	K01176.

Family and domain databases

InterProⁱ	View protein in InterPro IPR013776. A-amylase_thermo. IPR015237. Alpha-amylase_C_pro. IPR006047. Glyco_hydro_13_cat_dom. IPR017853. Glycoside_hydrolase_SF.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00128. Alpha-amylase. 1 hit. PF09154. DUF1939. 1 hit.
PIRSFⁱ	PIRSF001021. Alph-amls_thrmst. 1 hit.
SMARTⁱ	View protein in SMART SM00642. Aamy. 1 hit.
SUPFAMⁱ	SSF51445. SSF51445. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	Q81YJ4_BACAN
Accessionⁱ	Q81YJ4Primary (citable) accession number: Q81YJ4 Secondary accession number(s): E9R8P4 , E9R8P5, Q6HVU0, Q6KQ03
Entry historyⁱ	Integrated into UniProtKB/TrEMBL:	June 1, 2003
	Last sequence update:	June 1, 2003
	Last modified:	September 27, 2017
	This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Unreviewed (UniProtKB/TrEMBL)

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteomeImported

UniProtKB - Q81YJ4 (Q81YJ4_BACAN)

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Alpha-amylase

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Functionⁱ

Sites

GO - Molecular functionⁱ

GO - Biological processⁱ

Keywordsⁱ

Protein family/group databases

Names & Taxonomyⁱ

PTM / Processingⁱ

Molecule processing

Interactionⁱ

Protein-protein interaction databases

Family & Domainsⁱ

Domains and Repeats

Keywords - Domainⁱ

Phylogenomic databases

Family and domain databases

Sequenceⁱ

Sequence databases

Genome annotation databases

Similar proteinsⁱ

Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q81YJ4 (Q81YJ4_BACAN)

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Alpha-amylase

amyS

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Family & Domainsⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ