Q81XS5 (AMPA_BACAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	BA_5155, GBAA_5155, BAS4792

Organism

Bacillus anthracis

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_0000165718
Sites
	Active site	272	1	Potential
	Active site	346	1	Potential
	Metal binding	260	1	Manganese 2 By similarity
	Metal binding	265	1	Manganese 1 By similarity
	Metal binding	265	1	Manganese 2 By similarity
	Metal binding	283	1	Manganese 2 By similarity
	Metal binding	342	1	Manganese 1 By similarity
	Metal binding	344	1	Manganese 1 By similarity
	Metal binding	344	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81XS5 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: D400D6CC81FA31DF	FASTA	494	53,514
	10 20 30 40 50 60 MFQVQKELAS HEAVVVALFE EEKTSSFVQE LDKAFEGQLQ VLLEEKELST KKKAISKVHS 70 80 90 100 110 120 LGKTDVKRYY FVGLGKKESY TTETLRSALG KTFKTLQAAK VQDAAILLDS FVTEKLDAID 130 140 150 160 170 180 VAHIAAEVQG LGTYELQTYK SDKKDRVELE KFTAITAEDA QEIEAALTVG YVHGRATNSA 190 200 210 220 230 240 RTLVNMPPNV LTATKLAEYA VELAEKYDMD YKVLEKEEME ELGMGALLAV NQGSVEPPKM 250 260 270 280 290 300 IALIYKGKEE WTDVIGFVGK GITYDTGGYS LKPREGMVGM KGDMGGAAAV LGAMEIIGEL 310 320 330 340 350 360 RPEQNVIAVI PSTDNVVSGT AFKPDDVITS MSGKTIEVLN TDAEGRLALA DGITYAKKLG 370 380 390 400 410 420 ANYLIDVATL TGGVIVALGN HTTGAMTNNE ELFEQVLEAS METDESIWQL PIFDRDKERV 430 440 450 460 470 480 RNSKFADLNN SPGREGHAVM AGTFIGEFAE DTPWVHLDIA GTSESSGAHD LGPAGATGAM 490 VRTLATLVER FGEE

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP28827.1. AE017334 Genomic DNA. Translation: AAT34284.1. AE017225 Genomic DNA. Translation: AAT57085.1.
RefSeq	NP_847341.1. NC_003997.3. YP_021809.1. NC_007530.2. YP_031035.1. NC_005945.1.
3D structure databases
ProteinModelPortal	Q81XS5.
ModBase	Search...
Protein family/group databases
MEROPS	M17.010.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000012243; EBBACP00000011999; EBBACG00000012235. EBBACT00000014816; EBBACP00000014437; EBBACG00000014808. EBBACT00000022736; EBBACP00000022221; EBBACG00000022727.
GeneID	1084531. 2819349. 2849928.
GenomeReviews	Gene locus BA_5155 in contig AE016879_GR. Gene locus BAS4792 in contig AE017225_GR. Gene locus GBAA_5155 in contig AE017334_GR.
KEGG	ban:BA_5155. bar:GBAA_5155. bat:BAS4792.
TIGR	BA_5155. GBAA_5155.
Phylogenomic databases
GeneTree	EBGT00050000002613.
HOGENOM	HBG742580.
OMA	FMINLAT.
ProtClustDB	PRK00913.
Enzyme and pathway databases
BioCyc	BANT260799:BAS4792-MONOMER. BANT261594:GBAA5155-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BACAN

Accession

Primary (citable) accession number: Q81XS5
Secondary accession number(s): Q6HRK3, Q6KKW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	June 1, 2003
Last modified:	November 16, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program