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Q81XR2

- DAPF_BACAN

UniProt

Q81XR2 - DAPF_BACAN

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Bacillus anthracis
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.By similarity

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei14 – 141SubstrateBy similarity
    Binding sitei67 – 671SubstrateBy similarity
    Active sitei76 – 761Proton donor/acceptorBy similarity
    Binding sitei166 – 1661SubstrateBy similarity
    Sitei168 – 1681Important for catalytic activitySequence Analysis
    Binding sitei199 – 1991SubstrateBy similarity
    Sitei217 – 2171Important for catalytic activitySequence Analysis
    Active sitei226 – 2261Proton donor/acceptorBy similarity

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciANTHRA:DAPF-MONOMER.
    BANT260799:GJAJ-4884-MONOMER.
    BANT261594:GJ7F-5046-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimerase (EC:5.1.1.7)
    Short name:
    DAP epimerase
    Gene namesi
    Name:dapF
    Ordered Locus Names:BA_5170, GBAA_5170, BAS4806
    OrganismiBacillus anthracis
    Taxonomic identifieri1392 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
    ProteomesiUP000000427: Chromosome, UP000000594: Chromosome, UP000005639: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 288288Diaminopimelate epimerasePRO_1000011837Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi76 ↔ 226By similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    IntActiQ81XR2. 3 interactions.
    STRINGi198094.BA_5170.

    Structurei

    Secondary structure

    288
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi1 – 1111
    Beta strandi14 – 207
    Turni21 – 233
    Helixi28 – 303
    Helixi31 – 399
    Turni41 – 433
    Beta strandi48 – 547
    Beta strandi57 – 6711
    Turni76 – 794
    Helixi80 – 8910
    Beta strandi94 – 1029
    Beta strandi105 – 12622
    Helixi132 – 1343
    Beta strandi143 – 15210
    Beta strandi155 – 17218
    Helixi176 – 1783
    Turni181 – 1833
    Helixi184 – 1896
    Beta strandi199 – 2079
    Beta strandi210 – 2167
    Beta strandi218 – 2203
    Helixi227 – 23913
    Beta strandi249 – 2524
    Beta strandi257 – 2626
    Beta strandi268 – 2736
    Beta strandi275 – 28612

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2OTNX-ray2.40A/B1-288[»]
    ProteinModelPortaliQ81XR2.
    SMRiQ81XR2. Positions 1-287.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ81XR2.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni11 – 122SubstrateBy similarity
    Regioni76 – 783Substrate bindingBy similarity
    Regioni217 – 2182Substrate bindingBy similarity
    Regioni227 – 2282Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.Curated

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220466.
    KOiK01778.
    OMAiKMRIFNN.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q81XR2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQFSFTKMH GLGNSYIYVN MFEEQIPEED LALVAEKVSN INTGIGADGM    50
    ILICPSDVAP VKMRMFNNDG SEGKSCGNGL RCVAKYAYEH KLVEDTVFTI 100
    ETLAGIVTAE VTVEEGKVTL AKIDMGAPRL TRAEIPMLGE GETPFIRENF 150
    LYNNHRYAFT AVSMGNPHAV IFVDDVEQAP LTTLGPVLET HEMFPERVNV 200
    EFIEILNEEE MNFRVWERGS GVTQACGTGA CAAVVASILN GKMERGKEIT 250
    VHLAGGDLMI AWTEEGNVLM KGPAEVICRG VYEYKIEA 288
    Length:288
    Mass (Da):31,613
    Last modified:June 1, 2003 - v1
    Checksum:iF82364D2AC4202CA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016879 Genomic DNA. Translation: AAP28840.1.
    AE017225 Genomic DNA. Translation: AAT57099.1.
    AE017334 Genomic DNA. Translation: AAT70160.1.
    RefSeqiNP_847354.1. NC_003997.3.
    YP_031049.1. NC_005945.1.
    YP_052652.1. NC_007530.2.

    Genome annotation databases

    EnsemblBacteriaiAAP28840; AAP28840; BA_5170.
    AAT57099; AAT57099; BAS4806.
    AAT70160; AAT70160; GBAA_5170.
    GeneIDi1084119.
    2821103.
    2848459.
    KEGGiban:BA_5170.
    bar:GBAA_5170.
    bat:BAS4806.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016879 Genomic DNA. Translation: AAP28840.1 .
    AE017225 Genomic DNA. Translation: AAT57099.1 .
    AE017334 Genomic DNA. Translation: AAT70160.1 .
    RefSeqi NP_847354.1. NC_003997.3.
    YP_031049.1. NC_005945.1.
    YP_052652.1. NC_007530.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2OTN X-ray 2.40 A/B 1-288 [» ]
    ProteinModelPortali Q81XR2.
    SMRi Q81XR2. Positions 1-287.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q81XR2. 3 interactions.
    STRINGi 198094.BA_5170.

    Protocols and materials databases

    DNASUi 1084119.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP28840 ; AAP28840 ; BA_5170 .
    AAT57099 ; AAT57099 ; BAS4806 .
    AAT70160 ; AAT70160 ; GBAA_5170 .
    GeneIDi 1084119.
    2821103.
    2848459.
    KEGGi ban:BA_5170.
    bar:GBAA_5170.
    bat:BAS4806.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220466.
    KOi K01778.
    OMAi KMRIFNN.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci ANTHRA:DAPF-MONOMER.
    BANT260799:GJAJ-4884-MONOMER.
    BANT261594:GJ7F-5046-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q81XR2.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
      Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
      , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
      Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ames / isolate Porton.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ames ancestor.
    3. "Complete genome sequence of Bacillus anthracis Sterne."
      Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
      Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sterne.
    4. "Crystal structure of the catalytically active form of diamin epimerase from Bacillus anthracis."
      Matho M.H., Fukuda K., Santelli E., Jaroszewski L., Liddington R.C., Roper D.
      Submitted (FEB-2007) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.49 ANGSTROMS), SUBUNIT.

    Entry informationi

    Entry nameiDAPF_BACAN
    AccessioniPrimary (citable) accession number: Q81XR2
    Secondary accession number(s): Q6F086, Q6HRI9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3