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Q81XR2

- DAPF_BACAN

UniProt

Q81XR2 - DAPF_BACAN

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Protein
Diaminopimelate epimerase
Gene
dapF, BA_5170, GBAA_5170, BAS4806
Organism
Bacillus anthracis
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan By similarity.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141Substrate By similarity
Binding sitei67 – 671Substrate By similarity
Active sitei76 – 761Proton donor/acceptor By similarity
Binding sitei166 – 1661Substrate By similarity
Sitei168 – 1681Important for catalytic activity Reviewed prediction
Binding sitei199 – 1991Substrate By similarity
Sitei217 – 2171Important for catalytic activity Reviewed prediction
Active sitei226 – 2261Proton donor/acceptor By similarity

GO - Molecular functioni

  1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciANTHRA:DAPF-MONOMER.
BANT260799:GJAJ-4884-MONOMER.
BANT261594:GJ7F-5046-MONOMER.
UniPathwayiUPA00034; UER00025.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimerase (EC:5.1.1.7)
Short name:
DAP epimerase
Gene namesi
Name:dapF
Ordered Locus Names:BA_5170, GBAA_5170, BAS4806
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000000427: Chromosome, UP000000594: Chromosome, UP000005639: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288Diaminopimelate epimeraseUniRule annotation
PRO_1000011837Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi76 ↔ 226 By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiQ81XR2. 3 interactions.
STRINGi198094.BA_5170.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1 – 1111
Beta strandi14 – 207
Turni21 – 233
Helixi28 – 303
Helixi31 – 399
Turni41 – 433
Beta strandi48 – 547
Beta strandi57 – 6711
Turni76 – 794
Helixi80 – 8910
Beta strandi94 – 1029
Beta strandi105 – 12622
Helixi132 – 1343
Beta strandi143 – 15210
Beta strandi155 – 17218
Helixi176 – 1783
Turni181 – 1833
Helixi184 – 1896
Beta strandi199 – 2079
Beta strandi210 – 2167
Beta strandi218 – 2203
Helixi227 – 23913
Beta strandi249 – 2524
Beta strandi257 – 2626
Beta strandi268 – 2736
Beta strandi275 – 28612

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OTNX-ray2.40A/B1-288[»]
ProteinModelPortaliQ81XR2.
SMRiQ81XR2. Positions 1-287.

Miscellaneous databases

EvolutionaryTraceiQ81XR2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni11 – 122Substrate By similarity
Regioni76 – 783Substrate binding By similarity
Regioni217 – 2182Substrate binding By similarity
Regioni227 – 2282Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0253.
HOGENOMiHOG000220466.
KOiK01778.
OMAiKMRIFNN.
OrthoDBiEOG6ND0M5.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase.
InterProiIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81XR2-1 [UniParc]FASTAAdd to Basket

« Hide

MSQFSFTKMH GLGNSYIYVN MFEEQIPEED LALVAEKVSN INTGIGADGM    50
ILICPSDVAP VKMRMFNNDG SEGKSCGNGL RCVAKYAYEH KLVEDTVFTI 100
ETLAGIVTAE VTVEEGKVTL AKIDMGAPRL TRAEIPMLGE GETPFIRENF 150
LYNNHRYAFT AVSMGNPHAV IFVDDVEQAP LTTLGPVLET HEMFPERVNV 200
EFIEILNEEE MNFRVWERGS GVTQACGTGA CAAVVASILN GKMERGKEIT 250
VHLAGGDLMI AWTEEGNVLM KGPAEVICRG VYEYKIEA 288
Length:288
Mass (Da):31,613
Last modified:June 1, 2003 - v1
Checksum:iF82364D2AC4202CA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016879 Genomic DNA. Translation: AAP28840.1.
AE017225 Genomic DNA. Translation: AAT57099.1.
AE017334 Genomic DNA. Translation: AAT70160.1.
RefSeqiNP_847354.1. NC_003997.3.
YP_031049.1. NC_005945.1.
YP_052652.1. NC_007530.2.

Genome annotation databases

EnsemblBacteriaiAAP28840; AAP28840; BA_5170.
AAT57099; AAT57099; BAS4806.
AAT70160; AAT70160; GBAA_5170.
GeneIDi1084119.
2821103.
2848459.
KEGGiban:BA_5170.
bar:GBAA_5170.
bat:BAS4806.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016879 Genomic DNA. Translation: AAP28840.1 .
AE017225 Genomic DNA. Translation: AAT57099.1 .
AE017334 Genomic DNA. Translation: AAT70160.1 .
RefSeqi NP_847354.1. NC_003997.3.
YP_031049.1. NC_005945.1.
YP_052652.1. NC_007530.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2OTN X-ray 2.40 A/B 1-288 [» ]
ProteinModelPortali Q81XR2.
SMRi Q81XR2. Positions 1-287.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q81XR2. 3 interactions.
STRINGi 198094.BA_5170.

Protocols and materials databases

DNASUi 1084119.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP28840 ; AAP28840 ; BA_5170 .
AAT57099 ; AAT57099 ; BAS4806 .
AAT70160 ; AAT70160 ; GBAA_5170 .
GeneIDi 1084119.
2821103.
2848459.
KEGGi ban:BA_5170.
bar:GBAA_5170.
bat:BAS4806.

Phylogenomic databases

eggNOGi COG0253.
HOGENOMi HOG000220466.
KOi K01778.
OMAi KMRIFNN.
OrthoDBi EOG6ND0M5.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00025 .
BioCyci ANTHRA:DAPF-MONOMER.
BANT260799:GJAJ-4884-MONOMER.
BANT261594:GJ7F-5046-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q81XR2.

Family and domain databases

HAMAPi MF_00197. DAP_epimerase.
InterProi IPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view ]
PANTHERi PTHR31689. PTHR31689. 1 hit.
Pfami PF01678. DAP_epimerase. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR00652. DapF. 1 hit.
PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
    Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
    , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
    Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames / isolate Porton.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames ancestor.
  3. "Complete genome sequence of Bacillus anthracis Sterne."
    Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sterne.
  4. "Crystal structure of the catalytically active form of diamin epimerase from Bacillus anthracis."
    Matho M.H., Fukuda K., Santelli E., Jaroszewski L., Liddington R.C., Roper D.
    Submitted (FEB-2007) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.49 ANGSTROMS), SUBUNIT.

Entry informationi

Entry nameiDAPF_BACAN
AccessioniPrimary (citable) accession number: Q81XR2
Secondary accession number(s): Q6F086, Q6HRI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2003
Last modified: June 11, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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