ID   LDH3_BACAN              Reviewed;         316 AA.
AC   Q81XJ7; Q6HRC7; Q6KKP6;
DT   21-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   16-JUN-2009, entry version 50.
DE   RecName: Full=L-lactate dehydrogenase 3;
DE            Short=L-LDH 3;
DE            EC=1.1.1.27;
GN   Name=ldh3; Synonyms=ldh-3;
GN   OrderedLocusNames=BA_5240, GBAA_5240, BAS4869;
OS   Bacillus anthracis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1392;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ames / isolate Porton;
RX   MEDLINE=22608414; PubMed=12721629; DOI=10.1038/nature01586;
RA   Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T.,
RA   Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R.,
RA   Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M.,
RA   Kolonay J.F., Beanan M.J., Dodson R.J., Brinkac L.M., Gwinn M.L.,
RA   DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H.,
RA   Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D.,
RA   Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F.,
RA   Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C.,
RA   Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O.,
RA   Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M.,
RA   Hanna P.C., Kolstoe A.-B., Fraser C.M.;
RT   "The genome sequence of Bacillus anthracis Ames and comparison to
RT   closely related bacteria.";
RL   Nature 423:81-86(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ames ancestor;
RA   Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B.,
RA   Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L.,
RA   Fraser C.M.;
RT   "Bacillus anthracis comparative genomics.";
RL   Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sterne;
RA   Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K.,
RA   Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R.,
RA   Richardson P., Rubin E., Tice H.;
RT   "Complete genome sequence of Bacillus anthracis Sterne.";
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: (S)-lactate + NAD(+) = pyruvate + NADH.
CC   -!- PATHWAY: Fermentation; pyruvate fermentation to lactate; (S)-
CC       lactate from pyruvate: step 1/1.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. LDH family.
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DR   EMBL; AE016879; AAP28905.1; -; Genomic_DNA.
DR   EMBL; AE017334; AAT34368.1; -; Genomic_DNA.
DR   EMBL; AE017225; AAT57161.1; -; Genomic_DNA.
DR   RefSeq; NP_847419.1; -.
DR   RefSeq; YP_021893.1; -.
DR   RefSeq; YP_031111.1; -.
DR   HSSP; P00344; 1LDB.
DR   GeneID; 1084686; -.
DR   GeneID; 2814538; -.
DR   GeneID; 2848635; -.
DR   GenomeReviews; AE016879_GR; BA_5240.
DR   GenomeReviews; AE017225_GR; BAS4869.
DR   GenomeReviews; AE017334_GR; GBAA5240.
DR   KEGG; ban:BA5240; -.
DR   KEGG; bar:GBAA5240; -.
DR   KEGG; bat:BAS4869; -.
DR   TIGR; BA_5240; -.
DR   TIGR; GBAA_5240; -.
DR   HOGENOM; Q81XJ7; -.
DR   OMA; Q81XJ7; DIITYQV.
DR   BioCyc; BANT260799:BAS4869-MON; -.
DR   BioCyc; BANT261594:GBAA5240-MON; -.
DR   BRENDA; 1.1.1.27; 267517.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004459; F:L-lactate dehydrogenase activity; IEA:HAMAP.
DR   GO; GO:0019642; P:anaerobic glycolysis; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_00488; -; 1.
DR   InterPro; IPR001557; L-lactate/malate_DH.
DR   InterPro; IPR011304; L-lactate_DH.
DR   InterPro; IPR018177; L-lactate_DH_AS.
DR   InterPro; IPR001236; Lactate/malate_DH.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.90.110.10; lact_mal_DH; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   PRINTS; PR00086; LLDHDRGNASE.
DR   TIGRFAMs; TIGR01771; L-LDH-NAD; 1.
DR   PROSITE; PS00064; L_LDH; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Glycolysis; NAD; Oxidoreductase.
FT   CHAIN         1    316       L-lactate dehydrogenase 3.
FT                                /FTId=PRO_0000168315.
FT   NP_BIND      14     42       NAD (By similarity).
FT   ACT_SITE    178    178       Proton acceptor (By similarity).
FT   BINDING      91     91       Substrate (By similarity).
FT   BINDING     123    123       NAD or substrate (By similarity).
FT   BINDING     154    154       Substrate (By similarity).
FT   BINDING     233    233       Substrate (By similarity).
SQ   SEQUENCE   316 AA;  34771 MW;  575157416E72F03E CRC64;
     MKRHTRKIAI IGTGLVGSSC AYSIVNQGIC EELLLIDINH ERAVGEAMDL SHCINFTNTR
     TKVYAGSYED CKDMDIVIIT AGPAPKPGQS RLDTLGASAK IMESVVGGVM ESGFDGIFLL
     ASNPVDIITY QVWKLSGLPR NRVIGTGTSL DSSRLRTILS EMLHVDPRSI HGYSLGEHGD
     SQMVAWSHVT VGGKPILQIL EEQKERFGEI DLDEIVEKTA KAGWEIYKRK GTTYYGIGNS
     LAYIASSIFN DDHRVIAVSA ILDGEYGEYD ICTGVPAIIT RDGIREIVEL NLTEDEESRF
     AKSNDILRDY MKTIGY
//