Reviewed, UniProtKB/Swiss-Prot Q81XJ7 (LDH3_BACAN)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase 3
      Short name=L-LDH 3
    EC=1.1.1.27

Gene names

Name:	ldh3
Synonyms:	ldh-3
Ordered Locus Names:	BA_5240, GBAA5240, BAS4869

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD(+) = pyruvate + NADH.
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	L-lactate dehydrogenase 3		PRO_0000168315
Regions
	Nucleotide binding	14 – 42	29	NAD By similarity
Sites
	Active site	178	1	Proton acceptor By similarity
	Binding site	91	1	Substrate By similarity
	Binding site	123	1	NAD or substrate By similarity
	Binding site	154	1	Substrate By similarity
	Binding site	233	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81XJ7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 575157416E72F03E	FASTA	316	34,771
	10 20 30 40 50 60 MKRHTRKIAI IGTGLVGSSC AYSIVNQGIC EELLLIDINH ERAVGEAMDL SHCINFTNTR 70 80 90 100 110 120 TKVYAGSYED CKDMDIVIIT AGPAPKPGQS RLDTLGASAK IMESVVGGVM ESGFDGIFLL 130 140 150 160 170 180 ASNPVDIITY QVWKLSGLPR NRVIGTGTSL DSSRLRTILS EMLHVDPRSI HGYSLGEHGD 190 200 210 220 230 240 SQMVAWSHVT VGGKPILQIL EEQKERFGEI DLDEIVEKTA KAGWEIYKRK GTTYYGIGNS 250 260 270 280 290 300 LAYIASSIFN DDHRVIAVSA ILDGEYGEYD ICTGVPAIIT RDGIREIVEL NLTEDEESRF 310 AKSNDILRDY MKTIGY

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016879 Genomic DNA. Translation: AAP28905.1. AE017334 Genomic DNA. Translation: AAT34368.1. AE017225 Genomic DNA. Translation: AAT57161.1.
RefSeq	NP_847419.1. YP_021893.1. YP_031111.1.
3D structure databases
HSSP	HSSP built from PDB template 1LDB based on UniProtKB P00344.
ModBase	Search...
Genome annotation databases
GeneID	1084686. 2814538. 2848635.
GenomeReviews	Gene locus BA_5240 in contig AE016879_GR. Gene locus BAS4869 in contig AE017225_GR. Gene locus GBAA5240 in contig AE017334_GR.
KEGG	ban:BA5240. bar:GBAA5240. bat:BAS4869.
TIGR	BA_5240. GBAA5240.
Phylogenomic databases
HOGENOM	Q81XJ7.
Enzyme and pathway databases
BioCyc	BANT260799:BAS4869-MON. BANT261594:GBAA5240-MON.
Family and domain databases
HAMAP	MF_00488. [Tree]
InterPro	IPR001557. L-lactate/malate_DHase. IPR011304. L-lactate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH3_BACAN

Accession

Primary (citable) accession number: Q81XJ7
Secondary accession number(s): Q6HRC7, Q6KKP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2004
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)