Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q81X75 (PGK_BACAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase
EC=2.7.2.3
Gene names
Name:pgk
Ordered Locus Names:BA_5367, GBAA_5367, BAS4988
OrganismBacillus anthracis [Reference proteome] [HAMAP]
Taxonomic identifier1392 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length394 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00145

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00145.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoglycerate kinase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 394394Phosphoglycerate kinase HAMAP-Rule MF_00145
PRO_0000145899

Regions

Nucleotide binding350 – 3534ATP By similarity
Region21 – 233Substrate binding By similarity
Region59 – 624Substrate binding By similarity

Sites

Binding site361Substrate By similarity
Binding site1181Substrate By similarity
Binding site1511Substrate By similarity
Binding site2011ATP By similarity
Binding site2921ATP; via carbonyl oxygen By similarity
Binding site3231ATP By similarity

Amino acid modifications

Modified residue1831Phosphoserine By similarity
Modified residue2991Phosphothreonine By similarity

Secondary structure

1 .............................................................................. 394
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q81X75 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 0C383DEAA759658A

FASTA39442,298
        10         20         30         40         50         60 
MNKKSIRDVD LKGKRVFCRV DFNVPMKEGK ITDETRIRAA LPTIQYLVEQ GAKVILASHL 

        70         80         90        100        110        120 
GRPKGQAVEE LRLTPVAARL GELLGKDVKK ADEAFGPVAQ EMVAAMNEGD VLVLENVRFY 

       130        140        150        160        170        180 
AGEEKNDAEL AKEFAALADI FVNDAFGAAH RAHASTAGIA DYLPAVSGLL MEKELEVLGK 

       190        200        210        220        230        240 
ALSNPERPFT AIIGGAKVKD KIGLIRHLLD KVDNLIIGGG LAYTFVKALG HEIGLSLCED 

       250        260        270        280        290        300 
DKIELAKEFM QLAKEKGVNF YMPVDVVITE EFSETATTKI VGIDSIPSNW EGVDIGPKTR 

       310        320        330        340        350        360 
EIYADVIKNS KLVVWNGPMG VFEMTPFAEG TKAVGQALAD AEGTYSVIGG GDSAAAVEKF 

       370        380        390 
GMADKMSHIS TGGGASLEFM EGKELPGVVC LNDK

« Hide

References

[1]"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. expand/collapse author list , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Ames / isolate Porton.
[2]"The complete genome sequence of Bacillus anthracis Ames 'Ancestor'."
Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D., Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.
J. Bacteriol. 191:445-446(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Ames ancestor.
[3]"Complete genome sequence of Bacillus anthracis Sterne."
Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sterne.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE016879 Genomic DNA. Translation: AAP29027.1.
AE017334 Genomic DNA. Translation: AAT34502.1.
AE017225 Genomic DNA. Translation: AAT57277.1.
RefSeqNP_847541.1. NC_003997.3.
YP_022027.1. NC_007530.2.
YP_031227.1. NC_005945.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3UWDX-ray1.68A1-394[»]
ProteinModelPortalQ81X75.
SMRQ81X75. Positions 1-394.
ModBaseSearch...

Protein-protein interaction databases

IntActQ81X75. 2 interactions.
STRING198094.BA_5367.

Protocols and materials databases

DNASU1084905.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP29027; AAP29027; BA_5367.
AAT34502; AAT34502; GBAA_5367.
AAT57277; AAT57277; BAS4988.
GeneID1084905.
2818481.
2852195.
KEGGban:BA_5367.
bar:GBAA_5367.
bat:BAS4988.

Phylogenomic databases

eggNOGCOG0126.
HOGENOMHOG000227108.
KOK00927.
OMAKFGLADK.
ProtClustDBPRK00073.

Enzyme and pathway databases

BioCycBANT260799:GJAJ-5062-MONOMER.
BANT261594:GJ7F-5238-MONOMER.
UniPathwayUPA00109; UER00185.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. PGK. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGK_BACAN
AccessionPrimary (citable) accession number: Q81X75
Secondary accession number(s): Q6HR11, Q6KKD0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2003
Last modified: May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents