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Protein

Phosphoglycerate kinase

Gene

pgk

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk), Phosphoglycerate kinase (pgk), Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA1)
  4. Enolase (eno), Enolase (eno)
  5. Pyruvate kinase (pykA1), Pyruvate kinase (pyk), Pyruvate kinase (pyk2), Pyruvate kinase (ABW01_15615), Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36SubstrateUniRule annotation1
Binding sitei118SubstrateUniRule annotation1
Binding sitei151SubstrateUniRule annotation1
Binding sitei201ATPUniRule annotation1
Binding sitei292ATP; via carbonyl oxygenUniRule annotation1
Binding sitei323ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi350 – 353ATPUniRule annotation4

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphoglycerate kinase activity Source: TIGR

GO - Biological processi

  • glycolytic process Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciANTHRA:GFG-4116-MONOMER.
UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinaseUniRule annotation (EC:2.7.2.3UniRule annotation)
Gene namesi
Name:pgkUniRule annotation
Ordered Locus Names:BA_5367, GBAA_5367, BAS4988
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001458991 – 394Phosphoglycerate kinaseAdd BLAST394

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei183PhosphoserineUniRule annotation1
Modified residuei299PhosphothreonineUniRule annotation1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

IntActiQ81X75. 2 interactors.
STRINGi198094.BA_5367.

Structurei

Secondary structure

1394
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 8Combined sources3
Beta strandi15 – 19Combined sources5
Helixi35 – 39Combined sources5
Helixi41 – 49Combined sources9
Beta strandi53 – 57Combined sources5
Helixi69 – 71Combined sources3
Helixi74 – 84Combined sources11
Beta strandi94 – 96Combined sources3
Helixi97 – 104Combined sources8
Beta strandi111 – 113Combined sources3
Helixi117 – 119Combined sources3
Helixi121 – 125Combined sources5
Helixi128 – 135Combined sources8
Beta strandi139 – 143Combined sources5
Helixi146 – 148Combined sources3
Turni154 – 157Combined sources4
Helixi158 – 161Combined sources4
Beta strandi165 – 167Combined sources3
Helixi169 – 183Combined sources15
Beta strandi187 – 193Combined sources7
Helixi198 – 208Combined sources11
Turni209 – 211Combined sources3
Beta strandi213 – 217Combined sources5
Helixi222 – 228Combined sources7
Helixi240 – 242Combined sources3
Helixi243 – 256Combined sources14
Beta strandi259 – 261Combined sources3
Beta strandi264 – 271Combined sources8
Beta strandi278 – 282Combined sources5
Helixi283 – 285Combined sources3
Beta strandi291 – 295Combined sources5
Helixi297 – 308Combined sources12
Beta strandi311 – 317Combined sources7
Helixi325 – 327Combined sources3
Helixi329 – 340Combined sources12
Beta strandi345 – 348Combined sources4
Helixi351 – 359Combined sources9
Helixi363 – 365Combined sources3
Beta strandi367 – 369Combined sources3
Helixi374 – 380Combined sources7
Helixi386 – 389Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UWDX-ray1.68A1-394[»]
ProteinModelPortaliQ81X75.
SMRiQ81X75.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 23Substrate bindingUniRule annotation3
Regioni59 – 62Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105BZA. Bacteria.
COG0126. LUCA.
HOGENOMiHOG000227108.
KOiK00927.
OMAiEVLMVEN.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81X75-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKKSIRDVD LKGKRVFCRV DFNVPMKEGK ITDETRIRAA LPTIQYLVEQ
60 70 80 90 100
GAKVILASHL GRPKGQAVEE LRLTPVAARL GELLGKDVKK ADEAFGPVAQ
110 120 130 140 150
EMVAAMNEGD VLVLENVRFY AGEEKNDAEL AKEFAALADI FVNDAFGAAH
160 170 180 190 200
RAHASTAGIA DYLPAVSGLL MEKELEVLGK ALSNPERPFT AIIGGAKVKD
210 220 230 240 250
KIGLIRHLLD KVDNLIIGGG LAYTFVKALG HEIGLSLCED DKIELAKEFM
260 270 280 290 300
QLAKEKGVNF YMPVDVVITE EFSETATTKI VGIDSIPSNW EGVDIGPKTR
310 320 330 340 350
EIYADVIKNS KLVVWNGPMG VFEMTPFAEG TKAVGQALAD AEGTYSVIGG
360 370 380 390
GDSAAAVEKF GMADKMSHIS TGGGASLEFM EGKELPGVVC LNDK
Length:394
Mass (Da):42,298
Last modified:June 1, 2003 - v1
Checksum:i0C383DEAA759658A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP29027.1.
AE017334 Genomic DNA. Translation: AAT34502.1.
AE017225 Genomic DNA. Translation: AAT57277.1.
RefSeqiNP_847541.1. NC_003997.3.
WP_001036337.1. NZ_LHUO01000001.1.
YP_031227.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP29027; AAP29027; BA_5367.
AAT34502; AAT34502; GBAA_5367.
AAT57277; AAT57277; BAS4988.
GeneIDi1084905.
2852195.
KEGGiban:BA_5367.
bar:GBAA_5367.
bat:BAS4988.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP29027.1.
AE017334 Genomic DNA. Translation: AAT34502.1.
AE017225 Genomic DNA. Translation: AAT57277.1.
RefSeqiNP_847541.1. NC_003997.3.
WP_001036337.1. NZ_LHUO01000001.1.
YP_031227.1. NC_005945.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UWDX-ray1.68A1-394[»]
ProteinModelPortaliQ81X75.
SMRiQ81X75.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ81X75. 2 interactors.
STRINGi198094.BA_5367.

Protocols and materials databases

DNASUi1084905.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP29027; AAP29027; BA_5367.
AAT34502; AAT34502; GBAA_5367.
AAT57277; AAT57277; BAS4988.
GeneIDi1084905.
2852195.
KEGGiban:BA_5367.
bar:GBAA_5367.
bat:BAS4988.

Phylogenomic databases

eggNOGiENOG4105BZA. Bacteria.
COG0126. LUCA.
HOGENOMiHOG000227108.
KOiK00927.
OMAiEVLMVEN.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.
BioCyciANTHRA:GFG-4116-MONOMER.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGK_BACAN
AccessioniPrimary (citable) accession number: Q81X75
Secondary accession number(s): Q6HR11, Q6KKD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.