Q81WN7 (Q81WN7_BACAN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Dihydrodipicolinate synthase EMBL AAP27665.1
EC=4.2.1.52 EMBL AAP27665.1

Gene names

Name:	dapA2 EMBL AAP27665.1
Ordered Locus Names:	BA_3935, BAS3650, GBAA_3935 EMBL AAT33050.1

Organism

Bacillus anthracis

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. SAAS SAAS005263
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. SAAS SAAS005263
Subcellular location	Cytoplasm By similarity SAAS SAAS005263.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis SAAS SAAS005263 Lysine biosynthesis SAAS SAAS005263
Cellular component	Cytoplasm SAAS SAAS005263
Ligand	Schiff base SAAS SAAS005263
Molecular function	Lyase SAAS SAAS005263 EMBL AAP27665.1
Technical term	3D-structure PDB 1XKY PDB 1XL9 PDB 3HIJ Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q81WN7 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 3A6C4C3C4C92F021	FASTA	292	31,233
	10 20 30 40 50 60 MIDFGTIATA MVTPFDINGN IDFAKTTKLV NYLIDNGTTA IVVGGTTGES PTLTSEEKVA 70 80 90 100 110 120 LYRHVVSVVD KRVPVIAGTG SNNTHASIDL TKKATEVGVD AVMLVAPYYN KPSQEGMYQH 130 140 150 160 170 180 FKAIAESTPL PVMLYNVPGR SIVQISVDTV VRLSEIENIV AIKDAGGDVL TMTEIIEKTA 190 200 210 220 230 240 DDFAVYSGDD GLTLPAMAVG AKGIVSVASH VIGNEMQEMI AAFQAGEFKK AQKLHQLLVR 250 260 270 280 290 VTDSLFMAPS PTPVKTALQM VGLDVGSVRL PLLPLTEEER VTLQSVMQSI PR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Crystal structure of dihydrodipicolinate synthase (BA3935) from Bacillus anthracis at 1.94 A resolution." Blagova E., Levdikov V., Milioti N., Fogg M.J., Kalliomaa A.K., Brannigan J.A., Wilson K.S., Wilkinson A.J. Proteins 62:297-301(2006) [PubMed: 16287120] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.94 ANGSTROMS).
[3]	"Substrate-mediated stabilization of a tetrameric drug target reveals Achilles heel in anthrax." Voss J.E., Scally S.W., Taylor N.L., Atkinson S.C., Griffin M.D., Hutton C.A., Parker M.W., Alderton M.R., Gerrard J.A., Dobson R.C., Dogovski C., Perugini M.A. J. Biol. Chem. 285:5188-5195(2010) [PubMed: 19948665] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS).
[4]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.
[5]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE016879 Genomic DNA. Translation: AAP27665.1.
AE017334 Genomic DNA. Translation: AAT33050.1.
AE017225 Genomic DNA. Translation: AAT55952.1.

RefSeq

NP_846179.1. NC_003997.3.
YP_020575.1. NC_007530.2.
YP_029901.1. NC_005945.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XKY	X-ray	1.94	A/B/C/D	1-292	[»]
1XL9	X-ray	2.23	A/B/C/D	1-292	[»]
3HIJ	X-ray	2.15	A/B/C/D	1-292	[»]

ProteinModelPortal

Q81WN7.

SMR

Q81WN7. Positions 1-292.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBBACT00000010661; EBBACP00000010417; EBBACG00000010653.
EBBACT00000018434; EBBACP00000018055; EBBACG00000018425.
EBBACT00000023484; EBBACP00000022969; EBBACG00000023475.

GeneID

1086559.
2814544.
2849683.

GenomeReviews

Gene locus BA_3935 in contig AE016879_GR.
Gene locus BAS3650 in contig AE017225_GR.
Gene locus GBAA_3935 in contig AE017334_GR.

KEGG

ban:BA_3935.
bar:GBAA_3935.
bat:BAS3650.

TIGR

BA_3935.
GBAA3935.

Phylogenomic databases

GeneTree

EBGT00050000000637.

HOGENOM

HBG358848.

OMA

CEMEDSN.

ProtClustDB

PRK03170.

Family and domain databases

InterPro

IPR013785. Aldolase_TIM.
IPR002220. Dihydrodipicolinate_synth-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
IPR005263. Dihydrodipicolinate_synth_DapA.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

KO

K01714.

PANTHER

PTHR12128. DHDPS. 1 hit.

Pfam

PF00701. DHDPS. 1 hit.
[Graphical view]

PIRSF

PIRSF001365. DHDPS. 1 hit.

PRINTS

PR00146. DHPICSNTHASE.

TIGRFAMs

TIGR00674. DapA. 1 hit.

PROSITE

PS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q81WN7_BACAN

Accession

Primary (citable) accession number: Q81WN7
Secondary accession number(s): E9R5Y6 , E9R5Y7, Q6HUT7, Q6KP18

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2003
Last sequence update:	June 1, 2003
Last modified:	December 14, 2011
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)