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Q81WM8

- PNP_BACAN

UniProt

Q81WM8 - PNP_BACAN

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Bacillus anthracis
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi487 – 4871MagnesiumUniRule annotation
    Metal bindingi493 – 4931MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciANTHRA:PNP-MONOMER.
    BANT260799:GJAJ-3716-MONOMER.
    BANT261594:GJ7F-3832-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:BA_3944, GBAA_3944, BAS3658
    OrganismiBacillus anthracis
    Taxonomic identifieri1392 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
    ProteomesiUP000000427: Chromosome, UP000000594: Chromosome, UP000005639: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 712712Polyribonucleotide nucleotidyltransferasePRO_0000329511Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    IntActiQ81WM8. 2 interactions.
    STRINGi198094.BA_3944.

    Structurei

    3D structure databases

    ProteinModelPortaliQ81WM8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini554 – 61360KHUniRule annotationAdd
    BLAST
    Domaini623 – 69169S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218326.
    KOiK00962.
    OMAiISMEDTH.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q81WM8-1 [UniParc]FASTAAdd to Basket

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    MSQEKQVFSI DLAGRQLTVE TGQLAKQANG AVLVRYGDTA VLSTATASKE    50
    AKNVDFFPLT VNYEERLYAV GKIPGGFIKR EGRPSEKAIL ASRLIDRPIR 100
    PLFADGFRNE VQVVNIVMSV DQDCSSEMAA MLGSSLALSI SDIPFEGPIA 150
    GATVGRINGE FVINPTVEQQ EQSDIHLVVA GTKDAINMVE AGADQVPEET 200
    MLEAIMFGHD EIKRLIAFQE EIVQAVGKEK SEVKLYEVDA DLNQAVREMA 250
    EKDMHSAIQV HEKHAREDAI NEVKKRVIEH YEAQEADADT LGQVNEILYK 300
    IVKEEVRRLI TVEKIRPDGR KGDEIRPLAS EVGILSRTHG SGLFTRGQTQ 350
    ALSICTLGAL GDVQILDGLG VEESKRFMHH YNFPSFSVGE TRPMRGPGRR 400
    EIGHGALGER ALEPVIPSEK DFPYTVRLVS EVLESNGSTS QASICGSTLA 450
    MMDAGVPLKA PVAGIAMGLV KTGEHYTILS DIQGMEDHLG DMDFKVAGTA 500
    HGVTALQMDI KIDGLSREIL EEALQQAKVG RVHILNHMLS VIAEPRTELS 550
    AYAPKIITMT INPDKIRDVI GPSGKQINKI IEETGVKIDI EQDGTVFISS 600
    INQEMNDKAK KIIEDIVREV QVGEIYEGKV KRVEKFGAFV ELFSGKDGLV 650
    HISELALERV GKVEDVVKIG DVITVKVIEI DKQGRVNLSR KVLLKEEQEK 700
    EAAKEENKQE QQ 712
    Length:712
    Mass (Da):78,208
    Last modified:June 1, 2003 - v1
    Checksum:i9E1EF0E39280DA3D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016879 Genomic DNA. Translation: AAP27674.1.
    AE017334 Genomic DNA. Translation: AAT33058.1.
    AE017225 Genomic DNA. Translation: AAT55960.1.
    RefSeqiNP_846188.1. NC_003997.3.
    YP_020583.1. NC_007530.2.
    YP_029909.1. NC_005945.1.

    Genome annotation databases

    EnsemblBacteriaiAAP27674; AAP27674; BA_3944.
    AAT33058; AAT33058; GBAA_3944.
    AAT55960; AAT55960; BAS3658.
    GeneIDi1086877.
    2814451.
    2852317.
    KEGGiban:BA_3944.
    bar:GBAA_3944.
    bat:BAS3658.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016879 Genomic DNA. Translation: AAP27674.1 .
    AE017334 Genomic DNA. Translation: AAT33058.1 .
    AE017225 Genomic DNA. Translation: AAT55960.1 .
    RefSeqi NP_846188.1. NC_003997.3.
    YP_020583.1. NC_007530.2.
    YP_029909.1. NC_005945.1.

    3D structure databases

    ProteinModelPortali Q81WM8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q81WM8. 2 interactions.
    STRINGi 198094.BA_3944.

    Protocols and materials databases

    DNASUi 1086877.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP27674 ; AAP27674 ; BA_3944 .
    AAT33058 ; AAT33058 ; GBAA_3944 .
    AAT55960 ; AAT55960 ; BAS3658 .
    GeneIDi 1086877.
    2814451.
    2852317.
    KEGGi ban:BA_3944.
    bar:GBAA_3944.
    bat:BAS3658.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218326.
    KOi K00962.
    OMAi ISMEDTH.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci ANTHRA:PNP-MONOMER.
    BANT260799:GJAJ-3716-MONOMER.
    BANT261594:GJ7F-3832-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
      Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
      , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
      Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ames / isolate Porton.
    2. "Complete genome sequence of Bacillus anthracis Sterne."
      Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
      Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sterne.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ames ancestor.

    Entry informationi

    Entry nameiPNP_BACAN
    AccessioniPrimary (citable) accession number: Q81WM8
    Secondary accession number(s): Q6HUS9, Q6KP10
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3