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Protein

Proline--tRNA ligase 1

Gene

proS1

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.UniRule annotation

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligase 1UniRule annotation (EC:6.1.1.15UniRule annotation)
Alternative name(s):
Prolyl-tRNA synthetase 1UniRule annotation
Short name:
ProRS 1UniRule annotation
Gene namesi
Name:proS1UniRule annotation
Ordered Locus Names:BA_3957, GBAA_3957, BAS3670
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome
  • UP000000427 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002486421 – 566Proline--tRNA ligase 1Add BLAST566

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi260799.BAS3670

Structurei

3D structure databases

ProteinModelPortaliQ81WL6
SMRiQ81WL6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C90 Bacteria
COG0442 LUCA
HOGENOMiHOG000076894
KOiK01881
OMAiIQPAELW

Family and domain databases

CDDicd00779 ProRS_core_prok, 1 hit
Gene3Di3.40.50.800, 2 hits
3.90.960.10, 1 hit
HAMAPiMF_01569 Pro_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR006195 aa-tRNA-synth_II
IPR004154 Anticodon-bd
IPR036621 Anticodon-bd_dom_sf
IPR002316 Pro-tRNA-ligase_IIa
IPR004500 Pro-tRNA-synth_IIa_bac-type
IPR023717 Pro-tRNA-Synthase_IIa_type1
IPR033730 ProRS_core_prok
IPR036754 YbaK/aa-tRNA-synt-asso_dom_sf
IPR007214 YbaK/aa-tRNA-synth-assoc-dom
PfamiView protein in Pfam
PF03129 HGTP_anticodon, 1 hit
PF00587 tRNA-synt_2b, 1 hit
PF04073 tRNA_edit, 1 hit
PIRSFiPIRSF001535 ProRS_1, 1 hit
PRINTSiPR01046 TRNASYNTHPRO
SUPFAMiSSF55826 SSF55826, 1 hit
TIGRFAMsiTIGR00409 proS_fam_II, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

Q81WL6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQSMVFSPT LREVPADAEI KSHQLLLRAG FMRQNASGIY SFLPFGLKVL
60 70 80 90 100
HKVERIVREE MERAGAVELL MPAMQAAELW QESGRWYSYG SELMRMKDRN
110 120 130 140 150
AREFALGATH EEVITDLVRD EVKSYKKLPL TLYQIQTKFR DEQRPRFGLL
160 170 180 190 200
RGREFLMKDA YSFHATQESL DEVYDRLYKA YSNIFARCGL NFRAVIADSG
210 220 230 240 250
AMGGKDTHEF MVLSDVGEDT IAYSDTSDYA ANIEMAPVVA TYTKSDEAEK
260 270 280 290 300
ELEKVATPDQ KAIEEVSAFL NIEADKCIKS MVFKVDEKLV VVLVRGDHEV
310 320 330 340 350
NDVKVKNVYG ASVVELASHE EVKELLNCEV GSLGPIGVNG DIEIIADHAV
360 370 380 390 400
ASIVNGCSGA NEEGFHYVNV NPERDFKVSQ YTDLRFIQEG DQSPDGNGTI
410 420 430 440 450
LFARGIEVGH VFKLGTRYSE AMNATFLDEN GKTQPLIMGC YGIGVSRTVA
460 470 480 490 500
AIAEQFNDEN GLVWPKAVAP FHVHVIPVNM KSDAQREMGE NIYNSLQEQG
510 520 530 540 550
YEVLLDDRAE RAGVKFADAD LFGLPVRVTV GKKADEGIVE VKVRATGESE
560
EVKVEELQTY IANILK
Length:566
Mass (Da):63,179
Last modified:June 1, 2003 - v1
Checksum:i5953A84E3E4C1108
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA Translation: AAP27686.1
AE017225 Genomic DNA Translation: AAT55972.1
AE017334 Genomic DNA Translation: AAT33071.1
RefSeqiNP_846200.1, NC_003997.3
WP_000814312.1, NZ_PPES01000005.1
YP_029921.1, NC_005945.1

Genome annotation databases

EnsemblBacteriaiAAP27686; AAP27686; BA_3957
AAT33071; AAT33071; GBAA_3957
AAT55972; AAT55972; BAS3670
GeneIDi1086582
2852158
KEGGiban:BA_3957
bar:GBAA_3957
bat:BAS3670
PATRICifig|198094.11.peg.3927

Similar proteinsi

Entry informationi

Entry nameiSYP1_BACAN
AccessioniPrimary (citable) accession number: Q81WL6
Secondary accession number(s): Q6HUR7, Q6KNZ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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