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Q81WL6

- SYP1_BACAN

UniProt

Q81WL6 - SYP1_BACAN

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Protein
Proline--tRNA ligase 1
Gene
proS1, BA_3957, GBAA_3957, BAS3670
Organism
Bacillus anthracis
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity.UniRule annotation

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. proline-tRNA ligase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. prolyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBANT260799:GJAJ-3728-MONOMER.
BANT261594:GJ7F-3845-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligase 1 (EC:6.1.1.15)
Alternative name(s):
Prolyl-tRNA synthetase 1
Short name:
ProRS 1
Gene namesi
Name:proS1
Ordered Locus Names:BA_3957, GBAA_3957, BAS3670
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000000427: Chromosome, UP000000594: Chromosome, UP000005639: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 566566Proline--tRNA ligase 1UniRule annotation
PRO_0000248642Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi198094.BA_3957.

Structurei

3D structure databases

ProteinModelPortaliQ81WL6.
SMRiQ81WL6. Positions 1-565.

Family & Domainsi

Domaini

Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0442.
HOGENOMiHOG000076894.
KOiK01881.
OMAiCDTIEKL.
OrthoDBiEOG6TTVMR.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPiMF_01569. Pro_tRNA_synth_type1.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PANTHERiPTHR11451:SF3. PTHR11451:SF3. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81WL6-1 [UniParc]FASTAAdd to Basket

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MKQSMVFSPT LREVPADAEI KSHQLLLRAG FMRQNASGIY SFLPFGLKVL    50
HKVERIVREE MERAGAVELL MPAMQAAELW QESGRWYSYG SELMRMKDRN 100
AREFALGATH EEVITDLVRD EVKSYKKLPL TLYQIQTKFR DEQRPRFGLL 150
RGREFLMKDA YSFHATQESL DEVYDRLYKA YSNIFARCGL NFRAVIADSG 200
AMGGKDTHEF MVLSDVGEDT IAYSDTSDYA ANIEMAPVVA TYTKSDEAEK 250
ELEKVATPDQ KAIEEVSAFL NIEADKCIKS MVFKVDEKLV VVLVRGDHEV 300
NDVKVKNVYG ASVVELASHE EVKELLNCEV GSLGPIGVNG DIEIIADHAV 350
ASIVNGCSGA NEEGFHYVNV NPERDFKVSQ YTDLRFIQEG DQSPDGNGTI 400
LFARGIEVGH VFKLGTRYSE AMNATFLDEN GKTQPLIMGC YGIGVSRTVA 450
AIAEQFNDEN GLVWPKAVAP FHVHVIPVNM KSDAQREMGE NIYNSLQEQG 500
YEVLLDDRAE RAGVKFADAD LFGLPVRVTV GKKADEGIVE VKVRATGESE 550
EVKVEELQTY IANILK 566
Length:566
Mass (Da):63,179
Last modified:June 1, 2003 - v1
Checksum:i5953A84E3E4C1108
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016879 Genomic DNA. Translation: AAP27686.1.
AE017225 Genomic DNA. Translation: AAT55972.1.
AE017334 Genomic DNA. Translation: AAT33071.1.
RefSeqiNP_846200.1. NC_003997.3.
YP_020596.1. NC_007530.2.
YP_029921.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP27686; AAP27686; BA_3957.
AAT33071; AAT33071; GBAA_3957.
AAT55972; AAT55972; BAS3670.
GeneIDi1086582.
2819521.
2852158.
KEGGiban:BA_3957.
bar:GBAA_3957.
bat:BAS3670.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016879 Genomic DNA. Translation: AAP27686.1 .
AE017225 Genomic DNA. Translation: AAT55972.1 .
AE017334 Genomic DNA. Translation: AAT33071.1 .
RefSeqi NP_846200.1. NC_003997.3.
YP_020596.1. NC_007530.2.
YP_029921.1. NC_005945.1.

3D structure databases

ProteinModelPortali Q81WL6.
SMRi Q81WL6. Positions 1-565.
ModBasei Search...

Protein-protein interaction databases

STRINGi 198094.BA_3957.

Protocols and materials databases

DNASUi 1086582.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP27686 ; AAP27686 ; BA_3957 .
AAT33071 ; AAT33071 ; GBAA_3957 .
AAT55972 ; AAT55972 ; BAS3670 .
GeneIDi 1086582.
2819521.
2852158.
KEGGi ban:BA_3957.
bar:GBAA_3957.
bat:BAS3670.

Phylogenomic databases

eggNOGi COG0442.
HOGENOMi HOG000076894.
KOi K01881.
OMAi CDTIEKL.
OrthoDBi EOG6TTVMR.

Enzyme and pathway databases

BioCyci BANT260799:GJAJ-3728-MONOMER.
BANT261594:GJ7F-3845-MONOMER.

Family and domain databases

Gene3Di 3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPi MF_01569. Pro_tRNA_synth_type1.
InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view ]
PANTHERi PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfami PF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view ]
PRINTSi PR01046. TRNASYNTHPRO.
SUPFAMi SSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsi TIGR00409. proS_fam_II. 1 hit.
PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
    Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
    , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
    Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames / isolate Porton.
  2. "Complete genome sequence of Bacillus anthracis Sterne."
    Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sterne.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames ancestor.

Entry informationi

Entry nameiSYP1_BACAN
AccessioniPrimary (citable) accession number: Q81WL6
Secondary accession number(s): Q6HUR7, Q6KNZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi