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Q81WL6

- SYP1_BACAN

UniProt

Q81WL6 - SYP1_BACAN

Protein

Proline--tRNA ligase 1

Gene

proS1

Organism
Bacillus anthracis
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.UniRule annotation

    Catalytic activityi

    ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. proline-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. prolyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBANT260799:GJAJ-3728-MONOMER.
    BANT261594:GJ7F-3845-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proline--tRNA ligase 1UniRule annotation (EC:6.1.1.15UniRule annotation)
    Alternative name(s):
    Prolyl-tRNA synthetase 1UniRule annotation
    Short name:
    ProRS 1UniRule annotation
    Gene namesi
    Name:proS1UniRule annotation
    Ordered Locus Names:BA_3957, GBAA_3957, BAS3670
    OrganismiBacillus anthracis
    Taxonomic identifieri1392 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
    ProteomesiUP000000427: Chromosome, UP000000594: Chromosome, UP000005639: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 566566Proline--tRNA ligase 1PRO_0000248642Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi198094.BA_3957.

    Structurei

    3D structure databases

    ProteinModelPortaliQ81WL6.
    SMRiQ81WL6. Positions 1-565.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.UniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0442.
    HOGENOMiHOG000076894.
    KOiK01881.
    OMAiCDTIEKL.
    OrthoDBiEOG6TTVMR.

    Family and domain databases

    Gene3Di3.40.50.800. 1 hit.
    3.90.960.10. 1 hit.
    HAMAPiMF_01569. Pro_tRNA_synth_type1.
    InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023717. Pro-tRNA-Synthase_IIa_type1.
    IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
    [Graphical view]
    PANTHERiPTHR11451:SF3. PTHR11451:SF3. 1 hit.
    PfamiPF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF04073. tRNA_edit. 1 hit.
    [Graphical view]
    PRINTSiPR01046. TRNASYNTHPRO.
    SUPFAMiSSF52954. SSF52954. 1 hit.
    SSF55826. SSF55826. 1 hit.
    TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q81WL6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKQSMVFSPT LREVPADAEI KSHQLLLRAG FMRQNASGIY SFLPFGLKVL    50
    HKVERIVREE MERAGAVELL MPAMQAAELW QESGRWYSYG SELMRMKDRN 100
    AREFALGATH EEVITDLVRD EVKSYKKLPL TLYQIQTKFR DEQRPRFGLL 150
    RGREFLMKDA YSFHATQESL DEVYDRLYKA YSNIFARCGL NFRAVIADSG 200
    AMGGKDTHEF MVLSDVGEDT IAYSDTSDYA ANIEMAPVVA TYTKSDEAEK 250
    ELEKVATPDQ KAIEEVSAFL NIEADKCIKS MVFKVDEKLV VVLVRGDHEV 300
    NDVKVKNVYG ASVVELASHE EVKELLNCEV GSLGPIGVNG DIEIIADHAV 350
    ASIVNGCSGA NEEGFHYVNV NPERDFKVSQ YTDLRFIQEG DQSPDGNGTI 400
    LFARGIEVGH VFKLGTRYSE AMNATFLDEN GKTQPLIMGC YGIGVSRTVA 450
    AIAEQFNDEN GLVWPKAVAP FHVHVIPVNM KSDAQREMGE NIYNSLQEQG 500
    YEVLLDDRAE RAGVKFADAD LFGLPVRVTV GKKADEGIVE VKVRATGESE 550
    EVKVEELQTY IANILK 566
    Length:566
    Mass (Da):63,179
    Last modified:June 1, 2003 - v1
    Checksum:i5953A84E3E4C1108
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016879 Genomic DNA. Translation: AAP27686.1.
    AE017225 Genomic DNA. Translation: AAT55972.1.
    AE017334 Genomic DNA. Translation: AAT33071.1.
    RefSeqiNP_846200.1. NC_003997.3.
    YP_020596.1. NC_007530.2.
    YP_029921.1. NC_005945.1.

    Genome annotation databases

    EnsemblBacteriaiAAP27686; AAP27686; BA_3957.
    AAT33071; AAT33071; GBAA_3957.
    AAT55972; AAT55972; BAS3670.
    GeneIDi1086582.
    2819521.
    2852158.
    KEGGiban:BA_3957.
    bar:GBAA_3957.
    bat:BAS3670.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016879 Genomic DNA. Translation: AAP27686.1 .
    AE017225 Genomic DNA. Translation: AAT55972.1 .
    AE017334 Genomic DNA. Translation: AAT33071.1 .
    RefSeqi NP_846200.1. NC_003997.3.
    YP_020596.1. NC_007530.2.
    YP_029921.1. NC_005945.1.

    3D structure databases

    ProteinModelPortali Q81WL6.
    SMRi Q81WL6. Positions 1-565.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198094.BA_3957.

    Protocols and materials databases

    DNASUi 1086582.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP27686 ; AAP27686 ; BA_3957 .
    AAT33071 ; AAT33071 ; GBAA_3957 .
    AAT55972 ; AAT55972 ; BAS3670 .
    GeneIDi 1086582.
    2819521.
    2852158.
    KEGGi ban:BA_3957.
    bar:GBAA_3957.
    bat:BAS3670.

    Phylogenomic databases

    eggNOGi COG0442.
    HOGENOMi HOG000076894.
    KOi K01881.
    OMAi CDTIEKL.
    OrthoDBi EOG6TTVMR.

    Enzyme and pathway databases

    BioCyci BANT260799:GJAJ-3728-MONOMER.
    BANT261594:GJ7F-3845-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.800. 1 hit.
    3.90.960.10. 1 hit.
    HAMAPi MF_01569. Pro_tRNA_synth_type1.
    InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023717. Pro-tRNA-Synthase_IIa_type1.
    IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
    [Graphical view ]
    PANTHERi PTHR11451:SF3. PTHR11451:SF3. 1 hit.
    Pfami PF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF04073. tRNA_edit. 1 hit.
    [Graphical view ]
    PRINTSi PR01046. TRNASYNTHPRO.
    SUPFAMi SSF52954. SSF52954. 1 hit.
    SSF55826. SSF55826. 1 hit.
    TIGRFAMsi TIGR00409. proS_fam_II. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
      Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
      , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
      Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ames / isolate Porton.
    2. "Complete genome sequence of Bacillus anthracis Sterne."
      Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
      Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sterne.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ames ancestor.

    Entry informationi

    Entry nameiSYP1_BACAN
    AccessioniPrimary (citable) accession number: Q81WL6
    Secondary accession number(s): Q6HUR7, Q6KNZ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3