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Q81WH6 (PRKC_BACAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase PrkC
Short name=Ser/Thr-protein kinase PrkC
EC=2.7.11.1
Gene names
Name:prkC
Ordered Locus Names:BA_4000, GBAA_4000, BAS3713
OrganismBacillus anthracis [Reference proteome] [HAMAP]
Taxonomic identifier1392 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length657 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein kinase that is responsible for triggering spore germination in response to muropeptides, signaling bacteria to exit dormancy. PrkC is thus a germination receptor that binds peptidoglycan fragments containing m-Dpm (meso-diaminopimelate), which act as spore germinants. Probably autophosphorylates and phosphorylates FusA (EF-G, elongation factor G); the latter modification is likely necessary for germination in response to peptidoglycan. Ref.4

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Homodimer By similarity.

Subcellular location

Spore core membrane; Single-pass type II membrane protein. Note: Is associated with the inner membrane of the spore By similarity.

Domain

The C-terminal extracellular domain containing the PASTA repeats binds peptidoglycan By similarity.

Disruption phenotype

Spores lacking this gene fail to germinate in the presence of peptidoglycan fragments. Ref.4

Miscellaneous

Ref.4 shows that peptidoglycan fragments serve as a novel mechanism of interspecies bacterial signaling that likely indicates the presence of growing bacteria and thus serve as a signal for dormant cells that growth-promoting conditions exist.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 3 PASTA domains.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 657657Serine/threonine-protein kinase PrkC
PRO_0000398661

Regions

Topological domain1 – 345345Cytoplasmic Potential
Transmembrane346 – 36621Helical; Potential
Topological domain367 – 657291Extracellular Potential
Domain11 – 282272Protein kinase
Domain365 – 43268PASTA 1
Domain433 – 49967PASTA 2
Domain500 – 56667PASTA 3
Nucleotide binding17 – 259ATP By similarity

Sites

Active site1341Proton acceptor By similarity
Binding site401ATP By similarity
Site401Required for activity By similarity

Amino acid modifications

Modified residue1621Phosphothreonine; by autocatalysis By similarity
Modified residue1631Phosphothreonine; by autocatalysis By similarity
Modified residue1651Phosphothreonine; by autocatalysis By similarity
Modified residue1671Phosphothreonine; by autocatalysis By similarity
Modified residue2141Phosphoserine; by autocatalysis By similarity
Modified residue2901Phosphothreonine; by autocatalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
Q81WH6 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 20788151AFAF5503

FASTA65774,153
        10         20         30         40         50         60 
MLIGKRLNDR YKLLKMIGGG GMANVYLAHD DILGRDVAVK ILRLDYSNNE EFIKRFHREA 

        70         80         90        100        110        120 
QSVTTLSHPN IVNMYDVGEE DGIYYLVMEY VPGQTLKQYI IERGMLPIGE ALDIMEQLTS 

       130        140        150        160        170        180 
AMAHAHHFEI VHRDIKPHNI LIRADGVIKV TDFGIATATS ATTITHTNSV LGSVHYLSPE 

       190        200        210        220        230        240 
QARGGIANKQ SDIYSLGIVM FELLTGRQPF SGESAVAIAL KHLQSEIPSP KRWNENIPQS 

       250        260        270        280        290        300 
VENIILKATA KDPFHRYQSA NAMKRDIETA LYPERINEQP FYIPEDMEAT KAIPIIQQEQ 

       310        320        330        340        350        360 
LFENVTDETI VLKGSKVDEQ IRKEETDLSK KKKRSNKWLK ILITTFLLLA IGITLALTVI 

       370        380        390        400        410        420 
PGFFIPKDVK VPDVAGMKYT TAVNTLVEKG FEVTEPNIVY TDDVETGDVI KTDPVAGRVV 

       430        440        450        460        470        480 
KENSKITIYQ SGGKKKSKMI DFTGKDLESI RTELEEKYKQ VTVYYIEDDR PKGAIVEQIP 

       490        500        510        520        530        540 
TSDQMVVEAE QELKIWVSKG PYQIRPGDFS RWTENSVTGY LNERKLTPDI KREYSDTVDK 

       550        560        570        580        590        600 
GLVISQSPKP GTPLKEGDKV TIIISEGPKP KVTKTVKVDN ISIPYESSII GEKKPQTIEI 

       610        620        630        640        650 
YKEDMQQKMD RPIETRTISE SATISLEFVI QEDTKGRYKI VRDGVTIIDK EVPYPTQ

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. expand/collapse author list , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Ames / isolate Porton.
[2]"Complete genome sequence of Bacillus anthracis Sterne."
Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sterne.
[3]"The complete genome sequence of Bacillus anthracis Ames 'Ancestor'."
Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D., Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.
J. Bacteriol. 191:445-446(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Ames ancestor.
[4]"A eukaryotic-like Ser/Thr kinase signals bacteria to exit dormancy in response to peptidoglycan fragments."
Shah I.M., Laaberki M.H., Popham D.L., Dworkin J.
Cell 135:486-496(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN PEPTIDOGLYCAN-DEPENDENT GERMINATION, DISRUPTION PHENOTYPE.
Strain: Sterne.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE016879 Genomic DNA. Translation: AAP27728.1.
AE017334 Genomic DNA. Translation: AAT33115.2.
AE017225 Genomic DNA. Translation: AAT56015.1.
RefSeqNP_846242.1. NC_003997.3.
YP_020640.2. NC_007530.2.
YP_029964.1. NC_005945.1.

3D structure databases

HSSPHSSP built from PDB template 1O6Y based on UniProtKB P71584.
ProteinModelPortalQ81WH6.
ModBaseSearch...

Protein-protein interaction databases

IntActQ81WH6. 1 interaction.
STRING198094.BA_4000.

PTM databases

PhosSiteP1004930.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP27728; AAP27728; BA_4000.
AAT33115; AAT33115; GBAA_4000.
AAT56015; AAT56015; BAS3713.
GeneID1086759.
2815918.
2848195.
KEGGban:BA_4000.
bar:GBAA_4000.
bat:BAS3713.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000037186.
KOK08884.
OMAVIQEGTK.
ProtClustDBCLSK887290.

Enzyme and pathway databases

BioCycBANT260799:GJAJ-3771-MONOMER.
BANT261594:GJ7F-3888-MONOMER.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR005543. PASTA.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF03793. PASTA. 3 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00740. PASTA. 3 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. Kinase_like. 1 hit.
PROSITEPS51178. PASTA. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePRKC_BACAN
AccessionPrimary (citable) accession number: Q81WH6
Secondary accession number(s): Q6HUM4, Q6KNV8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: June 1, 2003
Last modified: May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents