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Protein

Serine/threonine-protein kinase PrkC

Gene

prkC

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein kinase that is responsible for triggering spore germination in response to muropeptides, signaling bacteria to exit dormancy. PrkC is thus a germination receptor that binds peptidoglycan fragments containing m-Dpm (meso-diaminopimelate), which act as spore germinants. Probably autophosphorylates and phosphorylates FusA (EF-G, elongation factor G); the latter modification is likely necessary for germination in response to peptidoglycan.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40ATPPROSITE-ProRule annotation1
Sitei40Required for activityBy similarity1
Active sitei134Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 25ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • peptidoglycan binding Source: UniProtKB
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  • cellular response to peptidoglycan Source: UniProtKB
  • protein phosphorylation Source: UniProtKB
  • signal transduction Source: UniProtKB
  • spore germination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Germination

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PrkC (EC:2.7.11.1)
Short name:
Ser/Thr-protein kinase PrkC
Gene namesi
Name:prkC
Ordered Locus Names:BA_4000, GBAA_4000, BAS3713
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 345CytoplasmicSequence analysisAdd BLAST345
Transmembranei346 – 366HelicalSequence analysisAdd BLAST21
Topological domaini367 – 657ExtracellularSequence analysisAdd BLAST291

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Spores lacking this gene fail to germinate in the presence of peptidoglycan fragments.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003986611 – 657Serine/threonine-protein kinase PrkCAdd BLAST657

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei162Phosphothreonine; by autocatalysisBy similarity1
Modified residuei163Phosphothreonine; by autocatalysisBy similarity1
Modified residuei165Phosphothreonine; by autocatalysisBy similarity1
Modified residuei167Phosphothreonine; by autocatalysisBy similarity1
Modified residuei214Phosphoserine; by autocatalysisBy similarity1
Modified residuei290Phosphothreonine; by autocatalysisBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ81WH6. 1 interactor.
STRINGi198094.BA_4000.

Structurei

3D structure databases

ProteinModelPortaliQ81WH6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 282Protein kinasePROSITE-ProRule annotationAdd BLAST272
Domaini365 – 432PASTA 1PROSITE-ProRule annotationAdd BLAST68
Domaini433 – 499PASTA 2PROSITE-ProRule annotationAdd BLAST67
Domaini500 – 566PASTA 3PROSITE-ProRule annotationAdd BLAST67

Domaini

The C-terminal extracellular domain containing the PASTA repeats binds peptidoglycan.By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 3 PASTA domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D9P. Bacteria.
COG0515. LUCA.
COG2815. LUCA.
HOGENOMiHOG000037186.
KOiK12132.
OMAiDYNTGAK.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR005543. PASTA_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF03793. PASTA. 3 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00740. PASTA. 3 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51178. PASTA. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81WH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIGKRLNDR YKLLKMIGGG GMANVYLAHD DILGRDVAVK ILRLDYSNNE
60 70 80 90 100
EFIKRFHREA QSVTTLSHPN IVNMYDVGEE DGIYYLVMEY VPGQTLKQYI
110 120 130 140 150
IERGMLPIGE ALDIMEQLTS AMAHAHHFEI VHRDIKPHNI LIRADGVIKV
160 170 180 190 200
TDFGIATATS ATTITHTNSV LGSVHYLSPE QARGGIANKQ SDIYSLGIVM
210 220 230 240 250
FELLTGRQPF SGESAVAIAL KHLQSEIPSP KRWNENIPQS VENIILKATA
260 270 280 290 300
KDPFHRYQSA NAMKRDIETA LYPERINEQP FYIPEDMEAT KAIPIIQQEQ
310 320 330 340 350
LFENVTDETI VLKGSKVDEQ IRKEETDLSK KKKRSNKWLK ILITTFLLLA
360 370 380 390 400
IGITLALTVI PGFFIPKDVK VPDVAGMKYT TAVNTLVEKG FEVTEPNIVY
410 420 430 440 450
TDDVETGDVI KTDPVAGRVV KENSKITIYQ SGGKKKSKMI DFTGKDLESI
460 470 480 490 500
RTELEEKYKQ VTVYYIEDDR PKGAIVEQIP TSDQMVVEAE QELKIWVSKG
510 520 530 540 550
PYQIRPGDFS RWTENSVTGY LNERKLTPDI KREYSDTVDK GLVISQSPKP
560 570 580 590 600
GTPLKEGDKV TIIISEGPKP KVTKTVKVDN ISIPYESSII GEKKPQTIEI
610 620 630 640 650
YKEDMQQKMD RPIETRTISE SATISLEFVI QEDTKGRYKI VRDGVTIIDK

EVPYPTQ
Length:657
Mass (Da):74,153
Last modified:June 1, 2003 - v1
Checksum:i20788151AFAF5503
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP27728.1.
AE017334 Genomic DNA. Translation: AAT33115.2.
AE017225 Genomic DNA. Translation: AAT56015.1.
RefSeqiNP_846242.1. NC_003997.3.
WP_000904759.1. NZ_LHUO01000006.1.
YP_029964.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP27728; AAP27728; BA_4000.
AAT33115; AAT33115; GBAA_4000.
AAT56015; AAT56015; BAS3713.
GeneIDi1086759.
2848195.
KEGGiban:BA_4000.
bar:GBAA_4000.
bat:BAS3713.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP27728.1.
AE017334 Genomic DNA. Translation: AAT33115.2.
AE017225 Genomic DNA. Translation: AAT56015.1.
RefSeqiNP_846242.1. NC_003997.3.
WP_000904759.1. NZ_LHUO01000006.1.
YP_029964.1. NC_005945.1.

3D structure databases

ProteinModelPortaliQ81WH6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ81WH6. 1 interactor.
STRINGi198094.BA_4000.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP27728; AAP27728; BA_4000.
AAT33115; AAT33115; GBAA_4000.
AAT56015; AAT56015; BAS3713.
GeneIDi1086759.
2848195.
KEGGiban:BA_4000.
bar:GBAA_4000.
bat:BAS3713.

Phylogenomic databases

eggNOGiENOG4105D9P. Bacteria.
COG0515. LUCA.
COG2815. LUCA.
HOGENOMiHOG000037186.
KOiK12132.
OMAiDYNTGAK.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR005543. PASTA_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF03793. PASTA. 3 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00740. PASTA. 3 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51178. PASTA. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRKC_BACAN
AccessioniPrimary (citable) accession number: Q81WH6
Secondary accession number(s): Q6HUM4, Q6KNV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

PubMed:18984160 shows that peptidoglycan fragments serve as a novel mechanism of interspecies bacterial signaling that likely indicates the presence of growing bacteria and thus serve as a signal for dormant cells that growth-promoting conditions exist.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.