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Q81WH6

- PRKC_BACAN

UniProt

Q81WH6 - PRKC_BACAN

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Protein

Serine/threonine-protein kinase PrkC

Gene
prkC, BA_4000, GBAA_4000, BAS3713
Organism
Bacillus anthracis
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Protein kinase that is responsible for triggering spore germination in response to muropeptides, signaling bacteria to exit dormancy. PrkC is thus a germination receptor that binds peptidoglycan fragments containing m-Dpm (meso-diaminopimelate), which act as spore germinants. Probably autophosphorylates and phosphorylates FusA (EF-G, elongation factor G); the latter modification is likely necessary for germination in response to peptidoglycan.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401ATP By similarity
Sitei40 – 401Required for activity By similarity
Active sitei134 – 1341Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 259ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. penicillin binding Source: InterPro
  3. peptidoglycan binding Source: UniProtKB
  4. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. cellular response to peptidoglycan Source: UniProtKB
  2. protein phosphorylation Source: UniProtKB
  3. signal transduction Source: UniProtKB
  4. spore germination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Germination

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBANT260799:GJAJ-3771-MONOMER.
BANT261594:GJ7F-3888-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PrkC (EC:2.7.11.1)
Short name:
Ser/Thr-protein kinase PrkC
Gene namesi
Name:prkC
Ordered Locus Names:BA_4000, GBAA_4000, BAS3713
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000000427: Chromosome, UP000000594: Chromosome, UP000005639: Chromosome

Subcellular locationi

Spore core membrane; Single-pass type II membrane protein
Note: Is associated with the inner membrane of the spore By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 345345Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei346 – 36621Helical; Reviewed predictionAdd
BLAST
Topological domaini367 – 657291Extracellular Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Spores lacking this gene fail to germinate in the presence of peptidoglycan fragments.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 657657Serine/threonine-protein kinase PrkCPRO_0000398661Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei162 – 1621Phosphothreonine; by autocatalysis By similarity
Modified residuei163 – 1631Phosphothreonine; by autocatalysis By similarity
Modified residuei165 – 1651Phosphothreonine; by autocatalysis By similarity
Modified residuei167 – 1671Phosphothreonine; by autocatalysis By similarity
Modified residuei214 – 2141Phosphoserine; by autocatalysis By similarity
Modified residuei290 – 2901Phosphothreonine; by autocatalysis By similarity

Keywords - PTMi

Phosphoprotein

PTM databases

PhosSiteiP1004930.

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

IntActiQ81WH6. 1 interaction.
STRINGi198094.BA_4000.

Structurei

3D structure databases

ProteinModelPortaliQ81WH6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 282272Protein kinaseAdd
BLAST
Domaini365 – 43268PASTA 1Add
BLAST
Domaini433 – 49967PASTA 2Add
BLAST
Domaini500 – 56667PASTA 3Add
BLAST

Domaini

The C-terminal extracellular domain containing the PASTA repeats binds peptidoglycan By similarity.

Sequence similaritiesi

Contains 3 PASTA domains.

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000037186.
KOiK08884.
OMAiRANEAPL.
OrthoDBiEOG6B35XT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR005543. PASTA_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF03793. PASTA. 3 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00740. PASTA. 3 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51178. PASTA. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81WH6-1 [UniParc]FASTAAdd to Basket

« Hide

MLIGKRLNDR YKLLKMIGGG GMANVYLAHD DILGRDVAVK ILRLDYSNNE    50
EFIKRFHREA QSVTTLSHPN IVNMYDVGEE DGIYYLVMEY VPGQTLKQYI 100
IERGMLPIGE ALDIMEQLTS AMAHAHHFEI VHRDIKPHNI LIRADGVIKV 150
TDFGIATATS ATTITHTNSV LGSVHYLSPE QARGGIANKQ SDIYSLGIVM 200
FELLTGRQPF SGESAVAIAL KHLQSEIPSP KRWNENIPQS VENIILKATA 250
KDPFHRYQSA NAMKRDIETA LYPERINEQP FYIPEDMEAT KAIPIIQQEQ 300
LFENVTDETI VLKGSKVDEQ IRKEETDLSK KKKRSNKWLK ILITTFLLLA 350
IGITLALTVI PGFFIPKDVK VPDVAGMKYT TAVNTLVEKG FEVTEPNIVY 400
TDDVETGDVI KTDPVAGRVV KENSKITIYQ SGGKKKSKMI DFTGKDLESI 450
RTELEEKYKQ VTVYYIEDDR PKGAIVEQIP TSDQMVVEAE QELKIWVSKG 500
PYQIRPGDFS RWTENSVTGY LNERKLTPDI KREYSDTVDK GLVISQSPKP 550
GTPLKEGDKV TIIISEGPKP KVTKTVKVDN ISIPYESSII GEKKPQTIEI 600
YKEDMQQKMD RPIETRTISE SATISLEFVI QEDTKGRYKI VRDGVTIIDK 650
EVPYPTQ 657
Length:657
Mass (Da):74,153
Last modified:June 1, 2003 - v1
Checksum:i20788151AFAF5503
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016879 Genomic DNA. Translation: AAP27728.1.
AE017334 Genomic DNA. Translation: AAT33115.2.
AE017225 Genomic DNA. Translation: AAT56015.1.
RefSeqiNP_846242.1. NC_003997.3.
YP_020640.2. NC_007530.2.
YP_029964.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP27728; AAP27728; BA_4000.
AAT33115; AAT33115; GBAA_4000.
AAT56015; AAT56015; BAS3713.
GeneIDi1086759.
2815918.
2848195.
KEGGiban:BA_4000.
bar:GBAA_4000.
bat:BAS3713.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016879 Genomic DNA. Translation: AAP27728.1 .
AE017334 Genomic DNA. Translation: AAT33115.2 .
AE017225 Genomic DNA. Translation: AAT56015.1 .
RefSeqi NP_846242.1. NC_003997.3.
YP_020640.2. NC_007530.2.
YP_029964.1. NC_005945.1.

3D structure databases

ProteinModelPortali Q81WH6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q81WH6. 1 interaction.
STRINGi 198094.BA_4000.

PTM databases

PhosSitei P1004930.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP27728 ; AAP27728 ; BA_4000 .
AAT33115 ; AAT33115 ; GBAA_4000 .
AAT56015 ; AAT56015 ; BAS3713 .
GeneIDi 1086759.
2815918.
2848195.
KEGGi ban:BA_4000.
bar:GBAA_4000.
bat:BAS3713.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000037186.
KOi K08884.
OMAi RANEAPL.
OrthoDBi EOG6B35XT.

Enzyme and pathway databases

BioCyci BANT260799:GJAJ-3771-MONOMER.
BANT261594:GJ7F-3888-MONOMER.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR005543. PASTA_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF03793. PASTA. 3 hits.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00740. PASTA. 3 hits.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS51178. PASTA. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
    Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
    , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
    Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames / isolate Porton.
  2. "Complete genome sequence of Bacillus anthracis Sterne."
    Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sterne.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames ancestor.
  4. "A eukaryotic-like Ser/Thr kinase signals bacteria to exit dormancy in response to peptidoglycan fragments."
    Shah I.M., Laaberki M.H., Popham D.L., Dworkin J.
    Cell 135:486-496(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN PEPTIDOGLYCAN-DEPENDENT GERMINATION, DISRUPTION PHENOTYPE.
    Strain: Sterne.

Entry informationi

Entry nameiPRKC_BACAN
AccessioniPrimary (citable) accession number: Q81WH6
Secondary accession number(s): Q6HUM4, Q6KNV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: June 1, 2003
Last modified: September 3, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

1 Publication shows that peptidoglycan fragments serve as a novel mechanism of interspecies bacterial signaling that likely indicates the presence of growing bacteria and thus serve as a signal for dormant cells that growth-promoting conditions exist.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi