Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q81WH6

- PRKC_BACAN

UniProt

Q81WH6 - PRKC_BACAN

Protein

Serine/threonine-protein kinase PrkC

Gene

prkC

Organism
Bacillus anthracis
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Protein kinase that is responsible for triggering spore germination in response to muropeptides, signaling bacteria to exit dormancy. PrkC is thus a germination receptor that binds peptidoglycan fragments containing m-Dpm (meso-diaminopimelate), which act as spore germinants. Probably autophosphorylates and phosphorylates FusA (EF-G, elongation factor G); the latter modification is likely necessary for germination in response to peptidoglycan.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei40 – 401ATPPROSITE-ProRule annotation
    Sitei40 – 401Required for activityBy similarity
    Active sitei134 – 1341Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi17 – 259ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. penicillin binding Source: InterPro
    3. peptidoglycan binding Source: UniProtKB
    4. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. cellular response to peptidoglycan Source: UniProtKB
    2. protein phosphorylation Source: UniProtKB
    3. signal transduction Source: UniProtKB
    4. spore germination Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Germination

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBANT260799:GJAJ-3771-MONOMER.
    BANT261594:GJ7F-3888-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase PrkC (EC:2.7.11.1)
    Short name:
    Ser/Thr-protein kinase PrkC
    Gene namesi
    Name:prkC
    Ordered Locus Names:BA_4000, GBAA_4000, BAS3713
    OrganismiBacillus anthracis
    Taxonomic identifieri1392 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
    ProteomesiUP000000427: Chromosome, UP000000594: Chromosome, UP000005639: Chromosome

    Subcellular locationi

    Spore core membrane; Single-pass type II membrane protein
    Note: Is associated with the inner membrane of the spore.By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Spores lacking this gene fail to germinate in the presence of peptidoglycan fragments.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 657657Serine/threonine-protein kinase PrkCPRO_0000398661Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei162 – 1621Phosphothreonine; by autocatalysisBy similarity
    Modified residuei163 – 1631Phosphothreonine; by autocatalysisBy similarity
    Modified residuei165 – 1651Phosphothreonine; by autocatalysisBy similarity
    Modified residuei167 – 1671Phosphothreonine; by autocatalysisBy similarity
    Modified residuei214 – 2141Phosphoserine; by autocatalysisBy similarity
    Modified residuei290 – 2901Phosphothreonine; by autocatalysisBy similarity

    Keywords - PTMi

    Phosphoprotein

    PTM databases

    PhosSiteiP1004930.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    IntActiQ81WH6. 1 interaction.
    STRINGi198094.BA_4000.

    Structurei

    3D structure databases

    ProteinModelPortaliQ81WH6.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 345345CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini367 – 657291ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei346 – 36621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini11 – 282272Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini365 – 43268PASTA 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini433 – 49967PASTA 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini500 – 56667PASTA 3PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The C-terminal extracellular domain containing the PASTA repeats binds peptidoglycan.By similarity

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 3 PASTA domains.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000037186.
    KOiK08884.
    OMAiRANEAPL.
    OrthoDBiEOG6B35XT.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR005543. PASTA_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF03793. PASTA. 3 hits.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00740. PASTA. 3 hits.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS51178. PASTA. 3 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q81WH6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLIGKRLNDR YKLLKMIGGG GMANVYLAHD DILGRDVAVK ILRLDYSNNE    50
    EFIKRFHREA QSVTTLSHPN IVNMYDVGEE DGIYYLVMEY VPGQTLKQYI 100
    IERGMLPIGE ALDIMEQLTS AMAHAHHFEI VHRDIKPHNI LIRADGVIKV 150
    TDFGIATATS ATTITHTNSV LGSVHYLSPE QARGGIANKQ SDIYSLGIVM 200
    FELLTGRQPF SGESAVAIAL KHLQSEIPSP KRWNENIPQS VENIILKATA 250
    KDPFHRYQSA NAMKRDIETA LYPERINEQP FYIPEDMEAT KAIPIIQQEQ 300
    LFENVTDETI VLKGSKVDEQ IRKEETDLSK KKKRSNKWLK ILITTFLLLA 350
    IGITLALTVI PGFFIPKDVK VPDVAGMKYT TAVNTLVEKG FEVTEPNIVY 400
    TDDVETGDVI KTDPVAGRVV KENSKITIYQ SGGKKKSKMI DFTGKDLESI 450
    RTELEEKYKQ VTVYYIEDDR PKGAIVEQIP TSDQMVVEAE QELKIWVSKG 500
    PYQIRPGDFS RWTENSVTGY LNERKLTPDI KREYSDTVDK GLVISQSPKP 550
    GTPLKEGDKV TIIISEGPKP KVTKTVKVDN ISIPYESSII GEKKPQTIEI 600
    YKEDMQQKMD RPIETRTISE SATISLEFVI QEDTKGRYKI VRDGVTIIDK 650
    EVPYPTQ 657
    Length:657
    Mass (Da):74,153
    Last modified:June 1, 2003 - v1
    Checksum:i20788151AFAF5503
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016879 Genomic DNA. Translation: AAP27728.1.
    AE017334 Genomic DNA. Translation: AAT33115.2.
    AE017225 Genomic DNA. Translation: AAT56015.1.
    RefSeqiNP_846242.1. NC_003997.3.
    YP_020640.2. NC_007530.2.
    YP_029964.1. NC_005945.1.

    Genome annotation databases

    EnsemblBacteriaiAAP27728; AAP27728; BA_4000.
    AAT33115; AAT33115; GBAA_4000.
    AAT56015; AAT56015; BAS3713.
    GeneIDi1086759.
    2815918.
    2848195.
    KEGGiban:BA_4000.
    bar:GBAA_4000.
    bat:BAS3713.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016879 Genomic DNA. Translation: AAP27728.1 .
    AE017334 Genomic DNA. Translation: AAT33115.2 .
    AE017225 Genomic DNA. Translation: AAT56015.1 .
    RefSeqi NP_846242.1. NC_003997.3.
    YP_020640.2. NC_007530.2.
    YP_029964.1. NC_005945.1.

    3D structure databases

    ProteinModelPortali Q81WH6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q81WH6. 1 interaction.
    STRINGi 198094.BA_4000.

    PTM databases

    PhosSitei P1004930.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP27728 ; AAP27728 ; BA_4000 .
    AAT33115 ; AAT33115 ; GBAA_4000 .
    AAT56015 ; AAT56015 ; BAS3713 .
    GeneIDi 1086759.
    2815918.
    2848195.
    KEGGi ban:BA_4000.
    bar:GBAA_4000.
    bat:BAS3713.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000037186.
    KOi K08884.
    OMAi RANEAPL.
    OrthoDBi EOG6B35XT.

    Enzyme and pathway databases

    BioCyci BANT260799:GJAJ-3771-MONOMER.
    BANT261594:GJ7F-3888-MONOMER.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR005543. PASTA_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF03793. PASTA. 3 hits.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00740. PASTA. 3 hits.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS51178. PASTA. 3 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
      Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
      , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
      Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ames / isolate Porton.
    2. "Complete genome sequence of Bacillus anthracis Sterne."
      Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
      Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sterne.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ames ancestor.
    4. "A eukaryotic-like Ser/Thr kinase signals bacteria to exit dormancy in response to peptidoglycan fragments."
      Shah I.M., Laaberki M.H., Popham D.L., Dworkin J.
      Cell 135:486-496(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN PEPTIDOGLYCAN-DEPENDENT GERMINATION, DISRUPTION PHENOTYPE.
      Strain: Sterne.

    Entry informationi

    Entry nameiPRKC_BACAN
    AccessioniPrimary (citable) accession number: Q81WH6
    Secondary accession number(s): Q6HUM4, Q6KNV8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    PubMed:18984160 shows that peptidoglycan fragments serve as a novel mechanism of interspecies bacterial signaling that likely indicates the presence of growing bacteria and thus serve as a signal for dormant cells that growth-promoting conditions exist.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3