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Protein

Orotate phosphoribosyltransferase

Gene

pyrE

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).UniRule annotation

Catalytic activityi

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from orotate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF), Orotidine 5'-phosphate decarboxylase (pyrF), Orotidine 5'-phosphate decarboxylase (pyrF), Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei945-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei985-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei1005-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei124OrotateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciANTHRA:PYRE-MONOMER.
UniPathwayiUPA00070; UER00119.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotate phosphoribosyltransferaseUniRule annotation (EC:2.4.2.10UniRule annotation)
Short name:
OPRTUniRule annotation
Short name:
OPRTaseUniRule annotation
Gene namesi
Name:pyrEUniRule annotation
Ordered Locus Names:BA_4021, GBAA_4021, BAS3733
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001106651 – 210Orotate phosphoribosyltransferaseAdd BLAST210

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi198094.BA_4021.

Structurei

Secondary structure

1210
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 11Combined sources11
Beta strandi14 – 17Combined sources4
Beta strandi30 – 35Combined sources6
Helixi37 – 42Combined sources6
Helixi44 – 61Combined sources18
Beta strandi67 – 70Combined sources4
Turni72 – 75Combined sources4
Helixi76 – 86Combined sources11
Beta strandi90 – 93Combined sources4
Beta strandi106 – 108Combined sources3
Beta strandi115 – 126Combined sources12
Helixi127 – 138Combined sources12
Beta strandi142 – 151Combined sources10
Helixi155 – 163Combined sources9
Beta strandi168 – 172Combined sources5
Helixi174 – 183Combined sources10
Helixi189 – 200Combined sources12
Beta strandi202 – 204Combined sources3
Helixi205 – 209Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3M3HX-ray1.75A1-210[»]
4RV4X-ray2.65A/B/C1-210[»]
ProteinModelPortaliQ81WF6.
SMRiQ81WF6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ81WF6.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni120 – 1285-phosphoribose 1-diphosphate bindingUniRule annotation9

Sequence similaritiesi

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG41065E1. Bacteria.
COG0461. LUCA.
HOGENOMiHOG000037975.
KOiK00762.
OMAiYSVYKMR.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE. 1 hit.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81WF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKEIASHLL EIGAVFLQPN DPFTWSSGMK SPIYCDNRLT LSYPKVRQTI
60 70 80 90 100
AAGLEELIKE HFPTVEVIAG TATAGIAHAA WVSDRMDLPM CYVRSKAKGH
110 120 130 140 150
GKGNQIEGKA EKGQKVVVVE DLISTGGSAI TCVEALREAG CEVLGIVSIF
160 170 180 190 200
TYELEAGKEK LEAANVASYS LSDYSALTEV AAEKGIIGQA ETKKLQEWRK
210
NPADEAWITA
Length:210
Mass (Da):22,714
Last modified:June 1, 2003 - v1
Checksum:i0FB8FABEBB5E54B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP27748.1.
AE017334 Genomic DNA. Translation: AAT33138.1.
AE017225 Genomic DNA. Translation: AAT56035.1.
RefSeqiNP_846262.1. NC_003997.3.
WP_000711466.1. NZ_LHUO01000006.1.
YP_029984.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP27748; AAP27748; BA_4021.
AAT33138; AAT33138; GBAA_4021.
AAT56035; AAT56035; BAS3733.
GeneIDi1086652.
2853071.
KEGGiban:BA_4021.
bar:GBAA_4021.
bat:BAS3733.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP27748.1.
AE017334 Genomic DNA. Translation: AAT33138.1.
AE017225 Genomic DNA. Translation: AAT56035.1.
RefSeqiNP_846262.1. NC_003997.3.
WP_000711466.1. NZ_LHUO01000006.1.
YP_029984.1. NC_005945.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3M3HX-ray1.75A1-210[»]
4RV4X-ray2.65A/B/C1-210[»]
ProteinModelPortaliQ81WF6.
SMRiQ81WF6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198094.BA_4021.

Protocols and materials databases

DNASUi1086652.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP27748; AAP27748; BA_4021.
AAT33138; AAT33138; GBAA_4021.
AAT56035; AAT56035; BAS3733.
GeneIDi1086652.
2853071.
KEGGiban:BA_4021.
bar:GBAA_4021.
bat:BAS3733.

Phylogenomic databases

eggNOGiENOG41065E1. Bacteria.
COG0461. LUCA.
HOGENOMiHOG000037975.
KOiK00762.
OMAiYSVYKMR.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.
BioCyciANTHRA:PYRE-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ81WF6.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE. 1 hit.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYRE_BACAN
AccessioniPrimary (citable) accession number: Q81WF6
Secondary accession number(s): Q6HUK4, Q6KNT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.