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Protein

Orotate phosphoribosyltransferase

Gene

pyrE

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).UniRule annotation

Catalytic activityi

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from orotate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF), Orotidine 5'-phosphate decarboxylase (pyrF), Orotidine 5'-phosphate decarboxylase (pyrF), Orotidine 5'-phosphate decarboxylase (pyrF), Orotidine 5'-phosphate decarboxylase (pyrF), Orotidine 5'-phosphate decarboxylase (pyrF), Orotidine 5'-phosphate decarboxylase (pyrF), Orotidine 5'-phosphate decarboxylase (pyrF), Orotidine 5'-phosphate decarboxylase (pyrF), Orotidine 5'-phosphate decarboxylase (pyrF), Orotidine 5'-phosphate decarboxylase (pyrF), Orotidine 5'-phosphate decarboxylase (pyrF), Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei945-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei985-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei1005-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei124OrotateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPyrimidine biosynthesis
LigandMagnesium

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119

Names & Taxonomyi

Protein namesi
Recommended name:
Orotate phosphoribosyltransferaseUniRule annotation (EC:2.4.2.10UniRule annotation)
Short name:
OPRTUniRule annotation
Short name:
OPRTaseUniRule annotation
Gene namesi
Name:pyrEUniRule annotation
Ordered Locus Names:BA_4021, GBAA_4021, BAS3733
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome
  • UP000000427 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001106651 – 210Orotate phosphoribosyltransferaseAdd BLAST210

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi260799.BAS3733

Structurei

Secondary structure

1210
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 11Combined sources11
Beta strandi14 – 17Combined sources4
Beta strandi30 – 35Combined sources6
Helixi37 – 42Combined sources6
Helixi44 – 61Combined sources18
Beta strandi67 – 70Combined sources4
Turni72 – 75Combined sources4
Helixi76 – 86Combined sources11
Beta strandi90 – 93Combined sources4
Beta strandi106 – 108Combined sources3
Beta strandi115 – 126Combined sources12
Helixi127 – 138Combined sources12
Beta strandi142 – 151Combined sources10
Helixi155 – 163Combined sources9
Beta strandi168 – 172Combined sources5
Helixi174 – 183Combined sources10
Helixi189 – 200Combined sources12
Beta strandi202 – 204Combined sources3
Helixi205 – 209Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3M3HX-ray1.75A1-210[»]
4RV4X-ray2.65A/B/C1-210[»]
ProteinModelPortaliQ81WF6
SMRiQ81WF6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ81WF6

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni120 – 1285-phosphoribose 1-diphosphate bindingUniRule annotation9

Sequence similaritiesi

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG41065E1 Bacteria
COG0461 LUCA
HOGENOMiHOG000037975
KOiK00762
OMAiHAAWVSE

Family and domain databases

CDDicd06223 PRTases_typeI, 1 hit
HAMAPiMF_01208 PyrE, 1 hit
InterProiView protein in InterPro
IPR023031 OPRT
IPR004467 Or_phspho_trans_dom
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
PfamiView protein in Pfam
PF00156 Pribosyltran, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
TIGRFAMsiTIGR00336 pyrE, 1 hit
PROSITEiView protein in PROSITE
PS00103 PUR_PYR_PR_TRANSFER, 1 hit

Sequencei

Sequence statusi: Complete.

Q81WF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKEIASHLL EIGAVFLQPN DPFTWSSGMK SPIYCDNRLT LSYPKVRQTI
60 70 80 90 100
AAGLEELIKE HFPTVEVIAG TATAGIAHAA WVSDRMDLPM CYVRSKAKGH
110 120 130 140 150
GKGNQIEGKA EKGQKVVVVE DLISTGGSAI TCVEALREAG CEVLGIVSIF
160 170 180 190 200
TYELEAGKEK LEAANVASYS LSDYSALTEV AAEKGIIGQA ETKKLQEWRK
210
NPADEAWITA
Length:210
Mass (Da):22,714
Last modified:June 1, 2003 - v1
Checksum:i0FB8FABEBB5E54B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA Translation: AAP27748.1
AE017334 Genomic DNA Translation: AAT33138.1
AE017225 Genomic DNA Translation: AAT56035.1
RefSeqiNP_846262.1, NC_003997.3
WP_000711466.1, NZ_PPES01000027.1
YP_029984.1, NC_005945.1

Genome annotation databases

EnsemblBacteriaiAAP27748; AAP27748; BA_4021
AAT33138; AAT33138; GBAA_4021
AAT56035; AAT56035; BAS3733
GeneIDi1086652
2853071
KEGGiban:BA_4021
banh:HYU01_19655
bar:GBAA_4021
bat:BAS3733
PATRICifig|198094.11.peg.3992

Similar proteinsi

Entry informationi

Entry nameiPYRE_BACAN
AccessioniPrimary (citable) accession number: Q81WF6
Secondary accession number(s): Q6HUK4, Q6KNT9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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