Reviewed, UniProtKB/Swiss-Prot Q81WF4 (PYRD_BACAN)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	BA_4023, GBAA_4023, BAS3735

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O2 = orotate + H2O2. HAMAP MF_00224
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6. HAMAP MF_00224
Subunit structure	Heterotetramer of 2 pyrK and 2 pyrD subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cytoplasm
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Dihydroorotate dehydrogenase HAMAP MF_00224		PRO_1000024123
Sites
	Active site	130	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81WF4-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 70E308F9028EAA27	FASTA	309	32,990
	10 20 30 40 50 60 MNRLQVELPG LSLKNPIIPA SGCFGFGREY AQFYDLSVLG SIMIKATTEQ PRYGNPTPRV 70 80 90 100 110 120 AETPGGMLNA IGLQNPGLEK VMNSELPWLE QFDLPIIANV AGSQAEDYVA VAKEISKAPN 130 140 150 160 170 180 VHALELNISC PNVKTGGIAF GTNPEIAADL TKRVKEVSEV PVYVKLSPNV ANIVEIAKAI 190 200 210 220 230 240 ENAGADGLTM INTLLGMRLD LKTAKPILAN RTGGLSGPAI KPVAIRMVHE VSQAVNIPII 250 260 270 280 290 300 GMGGIETAED VIEFFYAGAS AVAVGTANFI DPFVCPTIIE ELPALLDELG FDHISECQGR SWKQTCHSR

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.
[3]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016879 Genomic DNA. Translation: AAP27750.1. AE017334 Genomic DNA. Translation: AAT33140.1. AE017225 Genomic DNA. Translation: AAT56037.1.
RefSeq	NP_846264.1. YP_020665.1. YP_029986.1.
3D structure databases
HSSP	HSSP built from PDB template 1EP2 based on UniProtKB P54322.
SMR	Q81WF4. Positions 2-288.
ModBase	Search...
Genome annotation databases
GeneID	1086679. 2815297. 2850825.
GenomeReviews	Gene locus BA_4023 in contig AE016879_GR. Gene locus BAS3735 in contig AE017225_GR. Gene locus GBAA4023 in contig AE017334_GR.
KEGG	ban:BA4023. bar:GBAA4023. bat:BAS3735.
TIGR	BA_4023. GBAA_4023.
Phylogenomic databases
HOGENOM	Q81WF4.
OMA	Q81WF4. NSIGLQN.
Enzyme and pathway databases
BioCyc	BANT260799:BAS3735-MON. BANT261594:GBAA4023-MON.
BRENDA	1.3.3.1. 267517.
Family and domain databases
HAMAP	MF_00224. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005720. Dihydroorotate_DH_1_core. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01037. pyrD_sub1_fam. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_BACAN

Accession

Primary (citable) accession number: Q81WF4
Secondary accession number(s): Q6HUK2, Q6KNT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)