Q81WE9 (PYRB_BACAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	BA_4028, GBAA_4028, BAS3740

Organism

Bacillus anthracis

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP MF_00001
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Aspartate carbamoyltransferase HAMAP MF_00001		PRO_0000113089

Sequences

	Sequence		Length	Mass (Da)
	Q81WE9 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 7E4FE09447EE773E	FASTA	304	34,722
	10 20 30 40 50 60 MSHLLTMSEL SEVEISEILK DAEDFANGKE SKTTEQTFVA NLFFENSTRT RFSFEVAEKR 70 80 90 100 110 120 LGLDVLNFSA DASSVQKGET LYDTIRTLES IGTKAVVIRH EQDRYFDELK DQVNIPILNA 130 140 150 160 170 180 GDGCGNHPTQ CLLDLLTIKQ EFGRFEGLKI AIVGDVRHSR VARSNAEALT KLGATIYFAS 190 200 210 220 230 240 PEEWKDEDNT FGTYKPLDEL VPEVDVMMLL RVQHERHDHY ETDIMKEYHE KHGLTVEREK 250 260 270 280 290 300 RMKEGSIIMH PAPVNRDVEI ASELVECERS RIFKQMENGV YVRMAVLKRA LPNVLGGMKH ELFV

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP27755.1. AE017334 Genomic DNA. Translation: AAT33145.1. AE017225 Genomic DNA. Translation: AAT56042.1.
RefSeq	NP_846269.1. NC_003997.3. YP_020670.1. NC_007530.2. YP_029991.1. NC_005945.1.
3D structure databases
ProteinModelPortal	Q81WE9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q81WE9. 5 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000010378; EBBACP00000010134; EBBACG00000010370. EBBACT00000016180; EBBACP00000015801; EBBACG00000016172. EBBACT00000024430; EBBACP00000023915; EBBACG00000024421.
GeneID	1086640. 2819233. 2852925.
GenomeReviews	Gene locus BA_4028 in contig AE016879_GR. Gene locus BAS3740 in contig AE017225_GR. Gene locus GBAA_4028 in contig AE017334_GR.
KEGG	ban:BA_4028. bar:GBAA_4028. bat:BAS3740.
TIGR	BA_4028. GBAA_4028.
Phylogenomic databases
GeneTree	EBGT00050000002562.
HOGENOM	HBG579429.
OMA	LQRERMS.
ProtClustDB	PRK00856.
Enzyme and pathway databases
BioCyc	BANT260799:BAS3740-MONOMER. BANT261594:GBAA4028-MONOMER.
Family and domain databases
HAMAP	MF_00001. Asp_carb_tr. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
KO	K00609.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00670. Asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_BACAN

Accession

Primary (citable) accession number: Q81WE9
Secondary accession number(s): Q6HUJ7, Q6KNT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2003
Last modified:	November 16, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program