Q81WE4 (SYI1_BACAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase 1
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase 1
Short name=IleRS 1

Gene names

Name:	ileS1
Ordered Locus Names:	BA_4034, GBAA_4034, BAS3746

Organism

Bacillus anthracis

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	921 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02002
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02002
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_02002
Subunit structure	Monomer By similarity. HAMAP MF_02002
Subcellular location	Cytoplasm By similarity HAMAP MF_02002.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02002
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 921	921	Isoleucine--tRNA ligase 1 HAMAP MF_02002		PRO_0000098344
Regions
	Motif	57 – 67	11	"HIGH" region HAMAP MF_02002
	Motif	593 – 597	5	"KMSKS" region HAMAP MF_02002
Sites
	Metal binding	888	1	Zinc By similarity
	Metal binding	891	1	Zinc By similarity
	Metal binding	908	1	Zinc By similarity
	Metal binding	911	1	Zinc By similarity
	Binding site	552	1	Aminoacyl-adenylate By similarity
	Binding site	596	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81WE4 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 4B383BCCC606348B	FASTA	921	104,611
	10 20 30 40 50 60 MEYKNTLLMP KTEFPMRGNL PKREPAMQEK WAEMNIYETV QEHTKGRPLF VLHDGPPYAN 70 80 90 100 110 120 GDIHMGHALN KVLKDFIVRY KSMTGFCAPY VPGWDTHGLP IEQALTNKGV KRKEMTVAEF 130 140 150 160 170 180 RKLCAEYAYE QVERQREQFK RLGVRADWDN PYITLEPAYE AQQIKVFGDM AKKGYIYKGQ 190 200 210 220 230 240 KPVYWSPTSE SALAEAEIEY QDKKSASIYV AFSVKDGKNV LEGDEKYIIW TTTPWTLPAN 250 260 270 280 290 300 LGISVHPELE YAIVKVNDEK YIIASELFET VAKTLEWENA EVVKTVKGSE LEYTVAKHPF 310 320 330 340 350 360 YDRDSLVMLG DHVTTDAGTG CVHTAPGHGE DDFIVGKKYG LEVLCPVDDK GVLTEEAPGF 370 380 390 400 410 420 EGLFYDKANK PITEKLEEVG ALLKLTFITH SYPHDWRTKK PIIFRATAQW FASIEAFRKE 430 440 450 460 470 480 LLEAVAETKW VPAWGETRLH NMVRDRGDWC ISRQRAWGVP IPVFYAENGD PIITDETINH 490 500 510 520 530 540 VADLFREHGS NVWFEREAKD LLPEGFTHPG SPNGEFRKET DIMDVWFDSG SSHQAVLEER 550 560 570 580 590 600 DDLQRPADLY LEGSDQYRGW FNSSLSTAVA VTGKAPYKGV LSHGFVLDGE GRKMSKSIGN 610 620 630 640 650 660 IVVPKKIMDQ LGGDILRLWV SSVDYQSDVR ISDDILKQVA EVYRKIRNTF RFLLGNLDDF 670 680 690 700 710 720 KPSENTVAVA ELREVDRYML VKLNDLITKV KEAYETYDFA AVYHAIHNFC TIDLSSFYLD 730 740 750 760 770 780 FAKDILYIEG ANHEDRRAIQ TVLYDVLVAL TKLVTPILPH TADEVWPYIP GVTEESVQLT 790 800 810 820 830 840 DMPEAVQLDD AEALKTKWDA FMTLRDDVLK ALEVARNEKV IGKSLNASIT LYPTAEMKAM 850 860 870 880 890 900 LESINEDLKQ LFIVSEYKLG GMMEEAPADA PKYEHTAVVV AQATGETCER CWVVSETIGK 910 920 DAEHETLCER CATVVKENYV K

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP27760.1. AE017334 Genomic DNA. Translation: AAT33151.1. AE017225 Genomic DNA. Translation: AAT56048.1.
RefSeq	NP_846274.1. NC_003997.3. YP_020676.1. NC_007530.2. YP_029997.1. NC_005945.1.
3D structure databases
ProteinModelPortal	Q81WE4.
SMR	Q81WE4. Positions 2-883.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q81WE4. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000010132; EBBACP00000009888; EBBACG00000010124. EBBACT00000017895; EBBACP00000017516; EBBACG00000017886. EBBACT00000020216; EBBACP00000019701; EBBACG00000020207.
GeneID	1086630. 2818769. 2848181.
GenomeReviews	Gene locus BA_4034 in contig AE016879_GR. Gene locus BAS3746 in contig AE017225_GR. Gene locus GBAA_4034 in contig AE017334_GR.
KEGG	ban:BA_4034. bar:GBAA_4034. bat:BAS3746.
TIGR	BA_4034. GBAA_4034.
Phylogenomic databases
GeneTree	EBGT00070000031886.
HOGENOM	HBG577712.
OMA	KQVLTHG.
ProtClustDB	PRK05743.
Enzyme and pathway databases
BioCyc	BANT260799:BAS3746-MONOMER. BANT261594:GBAA4034-MONOMER.
Family and domain databases
HAMAP	MF_02002. Ile_tRNA_synth_type1. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-synt. IPR023585. Ile-tRNA-synt_type1. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. IPR010663. Znf_DNA_glyclase/IsotRNA_synth. [Graphical view]
Gene3D	G3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01870.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF06827. zf-FPG_IleRS. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. IleS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI1_BACAN

Accession

Primary (citable) accession number: Q81WE4
Secondary accession number(s): Q6HUJ1, Q6KNS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program