Q81WC7 (MURE_BACAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 72.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase EC=6.3.2.13 Alternative name(s): Meso-A2pm-adding enzyme Meso-diaminopimelate-adding enzyme UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase UDP-MurNAc-tripeptide synthetase UDP-N-acetylmuramyl-tripeptide synthetase | ||||
| Gene names |
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| Organism | Bacillus anthracis | ||||
| Taxonomic identifier | 1392 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group |
Protein attributes
| Sequence length | 491 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity. HAMAP MF_00208 |
| Catalytic activity | ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208 |
| Pathway | Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208 |
| Subcellular location | Cytoplasm By similarity HAMAP MF_00208. |
| Post-translational modification | Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity. HAMAP MF_00208 |
| Sequence similarities | Belongs to the MurCDEF family. MurE subfamily. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 491 | 491 | UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208 | PRO_0000101863 | |||||
Regions | |||||||||
| Nucleotide binding | 108 – 114 | 7 | ATP Potential | ||||||
| Region | 150 – 151 | 2 | UDP-MurNAc-L-Ala-D-Glu binding By similarity | ||||||
| Region | 407 – 410 | 4 | Meso-diaminopimelate binding By similarity | ||||||
| Motif | 407 – 410 | 4 | Meso-diaminopimelate recognition motif HAMAP MF_00208 | ||||||
Sites | |||||||||
| Binding site | 30 | 1 | UDP-MurNAc-L-Ala-D-Glu By similarity | ||||||
| Binding site | 149 | 1 | UDP-MurNAc-L-Ala-D-Glu By similarity | ||||||
| Binding site | 177 | 1 | UDP-MurNAc-L-Ala-D-Glu By similarity | ||||||
| Binding site | 185 | 1 | UDP-MurNAc-L-Ala-D-Glu By similarity | ||||||
| Binding site | 383 | 1 | Meso-diaminopimelate By similarity | ||||||
| Binding site | 457 | 1 | Meso-diaminopimelate; via carbonyl oxygen By similarity | ||||||
| Binding site | 461 | 1 | Meso-diaminopimelate By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 217 | 1 | N6-carboxylysine By similarity | ||||||
Sequences
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References
| [1] | "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. Fraser C.M.Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton. |
| [2] | "Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor. |
| [3] | "Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE016879 Genomic DNA. Translation: AAP27779.1. AE017334 Genomic DNA. Translation: AAT33170.1. AE017225 Genomic DNA. Translation: AAT56067.1. |
| RefSeq | NP_846293.1. NC_003997.3. YP_020695.1. NC_007530.2. YP_030016.1. NC_005945.1. |
3D structure databases | |
| ProteinModelPortal | Q81WC7. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBBACT00000008767; EBBACP00000008523; EBBACG00000008759. EBBACT00000016254; EBBACP00000015875; EBBACG00000016246. EBBACT00000023692; EBBACP00000023177; EBBACG00000023683. |
| GeneID | 1086243. 2815156. 2848185. |
| GenomeReviews | Gene locus BA_4053 in contig AE016879_GR. Gene locus BAS3765 in contig AE017225_GR. Gene locus GBAA_4053 in contig AE017334_GR. |
| KEGG | ban:BA_4053. bar:GBAA_4053. bat:BAS3765. |
| TIGR | BA_4053. GBAA_4053. |
Phylogenomic databases | |
| GeneTree | EBGT00050000000629. |
| HOGENOM | HBG602753. |
| OMA | IGTIANY. |
| ProtClustDB | PRK00139. |
Enzyme and pathway databases | |
| BioCyc | BANT260799:BAS3765-MONOMER. BANT261594:GBAA4053-MONOMER. |
Family and domain databases | |
| HAMAP | MF_00208. MurE. [Tree] |
| InterPro | IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view] |
| Gene3D | G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. |
| KO | K01928. |
| Pfam | PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view] |
| SUPFAM | SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit. |
| TIGRFAMs | TIGR01085. MurE. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | MURE_BACAN | ||||||||
| Accession | Primary (citable) accession number: Q81WC7 Secondary accession number(s): Q6HUH2, Q6KNQ8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with