Q81W03 (SYM1_BACAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase 1
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase 1
Short name=MetRS 1

Gene names

Name:	metG1
Synonyms:	metG, metS
Ordered Locus Names:	BA_0036, GBAA_0036, BAS0037

Organism

Bacillus anthracis

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	660 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_01228
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_01228
Subunit structure	Homodimer By similarity. HAMAP MF_01228
Subcellular location	Cytoplasm HAMAP MF_01228.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 660	660	Methionine--tRNA ligase 1 HAMAP MF_01228		PRO_0000139207
Regions
	Domain	560 – 660	101	tRNA-binding
	Motif	15 – 25	11	"HIGH" region HAMAP MF_01228
	Motif	310 – 314	5	"KMSKS" region HAMAP MF_01228
Sites
	Binding site	313	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81W03 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 5E9FB2B16EA70131	FASTA	660	75,125
	10 20 30 40 50 60 MTEENKSFYI TTPIYYPSGK LHIGHAYTTV AGDAMARYKC MQGYNVHYLT GTDEHGQKIQ 70 80 90 100 110 120 KKAEELNVTP QAYVDNIVAG IKELWEKMDI SYDDFIRTTE DRHKDVVEKI FKQLVDQGDI 130 140 150 160 170 180 YLDEYEGWYS VQDETFYTEH QLVDPIMEGD KVVGGKSPDS GHDVELVREE SYFFRMGKYV 190 200 210 220 230 240 DRLLKFYEDN PHFIQPESRK NEMINNFIKP GLEDLAVSRT SFDWGVRVPG NPKHVIYVWV 250 260 270 280 290 300 DALSNYITAL GYGTENEEMY KKFWPADVHL VGKEIVRFHT IYWPIILMAL DLPLPKKVFA 310 320 330 340 350 360 HGWILMKDGK MSKSKGNVVD PVILIDRYGL DALRYYLLRE VPFGSDGVFT PEGFVERINF 370 380 390 400 410 420 DLANDLGNLL NRTVAMIDKY FNGEIPAFKA NVTEFDETLV TFAKDTLKKV EEAMENMEFS 430 440 450 460 470 480 VALSSIWQLV SRTNKYIDET QPWVLAKDED DREKLASVMA HLAEVLRQTG IMLMPFLTVA 490 500 510 520 530 540 PSKMFAQLGL TDEAHTSWGS LSTIGCIPAG TKVEKGQPIF PRLEMDVEVA YIKEQMKASA 550 560 570 580 590 600 PKVEEKKEEE PKAEEITIDD FFKVELRVAE VLSAEPVKKA DKLLKIQLDL GTEKRQVVSG 610 620 630 640 650 660 IAKFYSPEDL KGKKVICVTN LKPVKLRGEL SQGMILAGEE NGVLSLASID QNIPNGTKIK

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP24091.1. AE017334 Genomic DNA. Translation: AAT29115.2. AE017225 Genomic DNA. Translation: AAT52375.1.
RefSeq	NP_842605.1. NC_003997.3. YP_016640.2. NC_007530.2. YP_026324.1. NC_005945.1.
3D structure databases
ProteinModelPortal	Q81W03.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q81W03. 5 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000012543; EBBACP00000012299; EBBACG00000012535. EBBACT00000014151; EBBACP00000013772; EBBACG00000014143. EBBACT00000024146; EBBACP00000023631; EBBACG00000024137.
GeneID	1084885. 2816080. 2848894.
GenomeReviews	Gene locus BA_0036 in contig AE016879_GR. Gene locus BAS0037 in contig AE017225_GR. Gene locus GBAA_0036 in contig AE017334_GR.
KEGG	ban:BA_0036. bar:GBAA_0036. bat:BAS0037.
TIGR	BA_0036. GBAA_0036.
Phylogenomic databases
GeneTree	EBGT00070000031891.
HOGENOM	HBG721667.
OMA	FDWGIKV.
ProtClustDB	PRK12267.
Enzyme and pathway databases
BioCyc	BANT260799:BAS0037-MONOMER. BANT261594:GBAA0036-MONOMER.
Family and domain databases
HAMAP	MF_01228. Met_tRNA_synth_type2. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR014758. Met-tRNA_synth. IPR023457. Met-tRNA_synth_2. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA-bd_dom. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01874.
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. MetG. 1 hit. TIGR00399. MetG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM1_BACAN

Accession

Primary (citable) accession number: Q81W03
Secondary accession number(s): Q6I506, Q6KYQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program