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Protein

Methionine--tRNA ligase 1

Gene

metG1

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.UniRule annotation

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei313 – 3131ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciANTHRA:METS-MONOMER.
BANT260799:GJAJ-44-MONOMER.
BANT261594:GJ7F-45-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase 1UniRule annotation (EC:6.1.1.10UniRule annotation)
Alternative name(s):
Methionyl-tRNA synthetase 1UniRule annotation
Short name:
MetRS 1UniRule annotation
Gene namesi
Name:metG1UniRule annotation
Synonyms:metG, metS
Ordered Locus Names:BA_0036, GBAA_0036, BAS0037
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 660660Methionine--tRNA ligase 1PRO_0000139207Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiQ81W03. 5 interactions.
STRINGi198094.BA_0036.

Structurei

3D structure databases

ProteinModelPortaliQ81W03.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini560 – 660101tRNA-bindingUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi15 – 2511"HIGH" regionAdd
BLAST
Motifi310 – 3145"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.UniRule annotation
Contains 1 tRNA-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0073. LUCA.
COG0143. LUCA.
HOGENOMiHOG000200401.
KOiK01874.
OMAiGHVMYVW.
OrthoDBiEOG6CVV9B.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR004495. Met-tRNA-synth_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81W03-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEENKSFYI TTPIYYPSGK LHIGHAYTTV AGDAMARYKC MQGYNVHYLT
60 70 80 90 100
GTDEHGQKIQ KKAEELNVTP QAYVDNIVAG IKELWEKMDI SYDDFIRTTE
110 120 130 140 150
DRHKDVVEKI FKQLVDQGDI YLDEYEGWYS VQDETFYTEH QLVDPIMEGD
160 170 180 190 200
KVVGGKSPDS GHDVELVREE SYFFRMGKYV DRLLKFYEDN PHFIQPESRK
210 220 230 240 250
NEMINNFIKP GLEDLAVSRT SFDWGVRVPG NPKHVIYVWV DALSNYITAL
260 270 280 290 300
GYGTENEEMY KKFWPADVHL VGKEIVRFHT IYWPIILMAL DLPLPKKVFA
310 320 330 340 350
HGWILMKDGK MSKSKGNVVD PVILIDRYGL DALRYYLLRE VPFGSDGVFT
360 370 380 390 400
PEGFVERINF DLANDLGNLL NRTVAMIDKY FNGEIPAFKA NVTEFDETLV
410 420 430 440 450
TFAKDTLKKV EEAMENMEFS VALSSIWQLV SRTNKYIDET QPWVLAKDED
460 470 480 490 500
DREKLASVMA HLAEVLRQTG IMLMPFLTVA PSKMFAQLGL TDEAHTSWGS
510 520 530 540 550
LSTIGCIPAG TKVEKGQPIF PRLEMDVEVA YIKEQMKASA PKVEEKKEEE
560 570 580 590 600
PKAEEITIDD FFKVELRVAE VLSAEPVKKA DKLLKIQLDL GTEKRQVVSG
610 620 630 640 650
IAKFYSPEDL KGKKVICVTN LKPVKLRGEL SQGMILAGEE NGVLSLASID
660
QNIPNGTKIK
Length:660
Mass (Da):75,125
Last modified:June 1, 2003 - v1
Checksum:i5E9FB2B16EA70131
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP24091.1.
AE017334 Genomic DNA. Translation: AAT29115.2.
AE017225 Genomic DNA. Translation: AAT52375.1.
RefSeqiNP_842605.1. NC_003997.3.
WP_000134128.1. NZ_LHUO01000018.1.
YP_026324.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP24091; AAP24091; BA_0036.
AAT29115; AAT29115; GBAA_0036.
AAT52375; AAT52375; BAS0037.
GeneIDi1084885.
2848894.
KEGGiban:BA_0036.
bar:GBAA_0036.
bat:BAS0037.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP24091.1.
AE017334 Genomic DNA. Translation: AAT29115.2.
AE017225 Genomic DNA. Translation: AAT52375.1.
RefSeqiNP_842605.1. NC_003997.3.
WP_000134128.1. NZ_LHUO01000018.1.
YP_026324.1. NC_005945.1.

3D structure databases

ProteinModelPortaliQ81W03.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ81W03. 5 interactions.
STRINGi198094.BA_0036.

Protocols and materials databases

DNASUi1084885.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP24091; AAP24091; BA_0036.
AAT29115; AAT29115; GBAA_0036.
AAT52375; AAT52375; BAS0037.
GeneIDi1084885.
2848894.
KEGGiban:BA_0036.
bar:GBAA_0036.
bat:BAS0037.

Phylogenomic databases

eggNOGiCOG0073. LUCA.
COG0143. LUCA.
HOGENOMiHOG000200401.
KOiK01874.
OMAiGHVMYVW.
OrthoDBiEOG6CVV9B.

Enzyme and pathway databases

BioCyciANTHRA:METS-MONOMER.
BANT260799:GJAJ-44-MONOMER.
BANT261594:GJ7F-45-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR004495. Met-tRNA-synth_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
    Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
    , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
    Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames / isolate Porton.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames ancestor.
  3. "Complete genome sequence of Bacillus anthracis Sterne."
    Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sterne.

Entry informationi

Entry nameiSYM1_BACAN
AccessioniPrimary (citable) accession number: Q81W03
Secondary accession number(s): Q6I506, Q6KYQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: June 1, 2003
Last modified: January 20, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.