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Protein

tRNA(Ile)-lysidine synthase

Gene

tilS

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.UniRule annotation

Catalytic activityi

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 35ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processtRNA processing
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile)-lysidine synthaseUniRule annotation (EC:6.3.4.19UniRule annotation)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthaseUniRule annotation
tRNA(Ile)-lysidine synthetaseUniRule annotation
Gene namesi
Name:tilSUniRule annotation
Ordered Locus Names:BA_0062, GBAA_0062, BAS0062
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome
  • UP000000427 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001816411 – 476tRNA(Ile)-lysidine synthaseAdd BLAST476

Proteomic databases

PRIDEiQ81VX7

Interactioni

Protein-protein interaction databases

STRINGi260799.BAS0062

Structurei

3D structure databases

ProteinModelPortaliQ81VX7
SMRiQ81VX7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similaritiesi

Belongs to the tRNA(Ile)-lysidine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D3U Bacteria
COG0037 LUCA
HOGENOMiHOG000236506
KOiK04075
OMAiAHCNFNL

Family and domain databases

CDDicd01992 PP-ATPase, 1 hit
Gene3Di3.40.50.620, 1 hit
HAMAPiMF_01161 tRNA_Ile_lys_synt, 1 hit
InterProiView protein in InterPro
IPR012796 Lysidine-tRNA-synth_C
IPR014729 Rossmann-like_a/b/a_fold
IPR011063 TilS/TtcA_N
IPR012094 tRNA_Ile_lys_synt
IPR012795 tRNA_Ile_lys_synt_N
IPR015262 tRNA_Ile_lys_synt_subst-bd
PANTHERiPTHR43033:SF1 PTHR43033:SF1, 1 hit
PfamiView protein in Pfam
PF01171 ATP_bind_3, 1 hit
PF09179 TilS, 1 hit
PF11734 TilS_C, 1 hit
SMARTiView protein in SMART
SM00977 TilS_C, 1 hit
TIGRFAMsiTIGR02433 lysidine_TilS_C, 1 hit
TIGR02432 lysidine_TilS_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q81VX7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDTFVEKVD DFVRQHDVLK ERSTIVVGVS GGPDSLALLY YLLEKRAAKQ
60 70 80 90 100
FEIVVAHVDH MFRGDESHED LQFVQDLCKG LGVICETIRI NVSQYQKQYG
110 120 130 140 150
MNAQVAAREC RYAFLERIMK KYDARYVALG HHGDDQVETI LMRLVRGSTP
160 170 180 190 200
KGYAGIAVKR PFHNGYLIRP LLGVTKEEIV NYCNELKIIP RIDPSNKKEV
210 220 230 240 250
YTRNRLRKYV LPHLKEENPQ VHEKFQKFSM QMQEDEAYLQ ELAFEKMNKV
260 270 280 290 300
ITKKSDKQIS LSIPTFESMS MPLQRRGIQL ILNYLYEYKI PSSLSSIHID
310 320 330 340 350
KVIEFFKRTQ PSGSLDFPGD LKIVRAYEEC SFGFKQEIVS PFLQDLSVPG
360 370 380 390 400
TITLSNGDKL VTEVSEDIPS DMNETVFVAK YNDISYPIRI RSRENGDRMS
410 420 430 440 450
IQGMNGTKKI KAIFIEAKVP REKREEWPVV CDASGNIIWL PLLKRSAFAI
460 470
SKETAKKDKY MIIHYKSKES SGRIMK
Length:476
Mass (Da):54,891
Last modified:June 1, 2003 - v1
Checksum:i432F463C58C14018
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA Translation: AAP24117.1
AE017334 Genomic DNA Translation: AAT29140.1
AE017225 Genomic DNA Translation: AAT52400.1
RefSeqiNP_842631.1, NC_003997.3
WP_000655474.1, NZ_PPES01000032.1
YP_026349.1, NC_005945.1

Genome annotation databases

EnsemblBacteriaiAAP24117; AAP24117; BA_0062
AAT29140; AAT29140; GBAA_0062
AAT52400; AAT52400; BAS0062
GeneIDi1086418
2851687
KEGGiban:BA_0062
banh:HYU01_00360
bar:GBAA_0062
bat:BAS0062
PATRICifig|198094.11.peg.59

Similar proteinsi

Entry informationi

Entry nameiTILS_BACAN
AccessioniPrimary (citable) accession number: Q81VX7
Secondary accession number(s): Q6I4Y1, Q6KYM5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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