Q81VV1 (SYC_BACAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	BA_0089, GBAA_0089, BAS0089

Organism

Bacillus anthracis

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	Cysteine--tRNA ligase HAMAP MF_00041		PRO_0000159343
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	266 – 270	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	209	1	Zinc By similarity
	Metal binding	234	1	Zinc By similarity
	Metal binding	238	1	Zinc By similarity
	Binding site	269	1	ATP By similarity
Amino acid modifications
	Modified residue	270	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81VV1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 8C7E74D385444991	FASTA	465	53,937
	10 20 30 40 50 60 MTIHIYNTLT RQKEEFTPLE ENKVKMYVCG PTVYNYIHIG NARPPMVFDT VRRYLEYKGY 70 80 90 100 110 120 DVQYVSNFTD VDDKLIKAAN ELGEDVPTIA DRFVEAYFED VTALGCKHAT VHPRVTENMD 130 140 150 160 170 180 IIIEFIQELV NKGYAYESEG DVYFRTKEFE GYGKLSHQPI ADLRHGARIE VGEKKQDPLD 190 200 210 220 230 240 FALWKAAKEG EIFWESPWGQ GRPGWHIECS AMARKYLGDT IDIHAGGQDL AFPHHENEIA 250 260 270 280 290 300 QSEALTGKTF ARYWMHNGYI NINNEKMSKS LGNFILVHDI IKQYDPQLIR FFMLSVHYRH 310 320 330 340 350 360 PINFSEELLQ STNNGLERIK TAYGNLKHRM ESSTDLTDHN EKWLADLEKF QTAFEEAMND 370 380 390 400 410 420 DFNTANAITE LYNVANHANQ YLLEEHTSTV VIEAYVKQLE TLFDILGLEL AQEELLDEEI 430 440 450 460 EALIQKRIEA RKNRDFALSD QIRDDLKDRN IILEDTAQGT RWKRG

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP24143.1. AE017334 Genomic DNA. Translation: AAT29168.1. AE017225 Genomic DNA. Translation: AAT52426.1.
RefSeq	NP_842657.1. NC_003997.3. YP_016693.1. NC_007530.2. YP_026375.1. NC_005945.1.
3D structure databases
ProteinModelPortal	Q81VV1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000008203; EBBACP00000007959; EBBACG00000008195. EBBACT00000016398; EBBACP00000016019; EBBACG00000016390. EBBACT00000022610; EBBACP00000022095; EBBACG00000022601.
GeneID	1085927. 2814899. 2849752.
GenomeReviews	Gene locus BA_0089 in contig AE016879_GR. Gene locus BAS0089 in contig AE017225_GR. Gene locus GBAA_0089 in contig AE017334_GR.
KEGG	ban:BA_0089. bar:GBAA_0089. bat:BAS0089.
TIGR	BA_0089. GBAA_0089.
Phylogenomic databases
GeneTree	EBGT00050000000801.
HOGENOM	HBG327651.
OMA	DFDALNM.
ProtClustDB	PRK00260.
Enzyme and pathway databases
BioCyc	BANT260799:BAS0089-MONOMER. BANT261594:GBAA0089-MONOMER.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_BACAN

Accession

Primary (citable) accession number: Q81VV1
Secondary accession number(s): Q6I4V5, Q6KYJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program