Q81VF6 (Q81VF6_BACAN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Alanine racemase EMBL AAT29333.1
EC=5.1.1.1 EMBL AAT29333.1

Submitted name:
Alanine racemase, spore-specific EMBL AAP24291.1
EC=5.1.1.1 EMBL AAP24291.1

Gene names

Name:	dal1 EMBL AAP24291.1
Ordered Locus Names:	BA_0252 EMBL AAP24291.1, BAS0238 EMBL AAT52574.1, GBAA_0252 EMBL AAT29333.1

Organism

Bacillus anthracis [Reference proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-alanine = D-alanine. SAAS SAAS020622
Cofactor	Pyridoxal phosphate By similarity. SAAS SAAS020622
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. SAAS SAAS020622 RuleBase RU004247
Sequence similarities	Belongs to the alanine racemase family. RuleBase RU004188

Ontologies

Keywords
Ligand	Pyridoxal phosphate SAAS SAAS020622
Molecular function	Isomerase SAAS SAAS020622 EMBL AAP24291.1
Technical term	3D-structure PDB 3HA1 PDB 2VD8 PDB 2VD9 Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	D-alanine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q81VF6 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 5B55B2C4920D6BA5	FASTA	389	43,662
	10 20 30 40 50 60 MEEAPFYRDT WVEVDLDAIY NNVTHIKEFI PSDVEIFAVV KGNAYGHDYV PVAKIALEAG 70 80 90 100 110 120 ATRLAVAFLD EALVLRRAGI TAPILVLGPS PPRDINVAAE NDVALTVFQK EWVDEAIKLW 130 140 150 160 170 180 DGSSTMKYHI NFDSGMGRIG IRERKELKGF LKSLEGAPFL ELEGVYTHFA TADEVETSYF 190 200 210 220 230 240 DKQYNTFLEQ LSWLKEFGVD PKFVHTANSA ATLRFQGITF NAVRIGIAMY GLSPSVEIRP 250 260 270 280 290 300 FLPFKLEPAL SLHTKVAHIK QVIKGDGISY NVTYRTKTEE WIATVAIGYA DGWLRRLQGF 310 320 330 340 350 360 EVLVNGKRVP IVGRVTMDQF MIHLPCEVPL GTKVTLIGRQ GDEYISATEV AEYSGTINYE 370 380 IITTISFRVP RIFIRNGKVV EVINYLNDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames EMBL AAP24291.1 and Ames / isolate Porton.
[2]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne EMBL AAT52574.1.
[3]	"Structures of an alanine racemase from Bacillus anthracis (BA0252) in the presence and absence of (R)-1-aminoethylphosphonic acid (L-Ala-P)." Au K., Ren J., Walter T.S., Harlos K., Nettleship J.E., Owens R.J., Stuart D.I., Esnouf R.M. Acta Crystallogr. F 64:327-333(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.47 ANGSTROMS).
[4]	"Biochemical and structural characterization of alanine racemase from Bacillus anthracis (Ames)." Counago R.M., Davlieva M., Strych U., Hill R.E., Krause K.L. BMC Struct. Biol. 9:53-53(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
[5]	"The complete genome sequence of Bacillus anthracis Ames "Ancestor"." Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D., Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A. J. Bacteriol. 191:445-446(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames Ancestor EMBL AAT29333.1 and Ames ancestor.
[6]	Joardar V., Shrivastava S., Brinkac L.M., Harkins D.M., Durkin A.S., Sutton G. Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: Ames EMBL AAP24291.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE016879 Genomic DNA. Translation: AAP24291.1.
AE017334 Genomic DNA. Translation: AAT29333.1.
AE017225 Genomic DNA. Translation: AAT52574.1.

RefSeq

NP_842805.1. NC_003997.3.
YP_016858.1. NC_007530.2.
YP_026523.1. NC_005945.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VD8	X-ray	1.47	A/B	1-389	[»]
2VD9	X-ray	2.10	A/B	1-389	[»]
3HA1	X-ray	1.95	A/B	1-389	[»]

SMR

Q81VF6. Positions 4-389.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q81VF6. 3 interactions.

STRING

198094.BA_0252.

Protocols and materials databases

DNASU

1087014.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAP24291; AAP24291; BA_0252.
AAT29333; AAT29333; GBAA_0252.
AAT52574; AAT52574; BAS0238.

GeneID

1087014.
2819200.
2848190.

KEGG

ban:BA_0252.
bar:GBAA_0252.
bat:BAS0238.

Phylogenomic databases

HOGENOM

HOG000031444.

KO

K01775.

OMA

DELGHHF.

ProtClustDB

CLSK915752.

Enzyme and pathway databases

BioCyc

BANT260799:GJAJ-260-MONOMER.
BANT261594:GJ7F-268-MONOMER.

UniPathway

UPA00042; UER00497.

Family and domain databases

Gene3D

2.40.37.10. 1 hit.

HAMAP

MF_01201. Ala_racemase.

InterPro

IPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
[Graphical view]

Pfam

PF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]

PRINTS

PR00992. ALARACEMASE.

SMART

SM01005. Ala_racemase_C. 1 hit.
[Graphical view]

SUPFAM

SSF50621. Racem_decarbox_C. 1 hit.

TIGRFAMs

TIGR00492. alr. 1 hit.

PROSITE

PS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q81VF6.

Entry information

Entry name

Q81VF6_BACAN

Accession

Primary (citable) accession number: Q81VF6
Secondary accession number(s): E9R9C7 , E9R9C8, Q6I4F7, Q6KY59

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2003
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)