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Q81VF6

- Q81VF6_BACAN

UniProt

Q81VF6 - Q81VF6_BACAN

Protein

Alanine racemase

Gene

dal1

Organism
Bacillus anthracis
Status
Unreviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.UniRule annotation

    Catalytic activityi

    L-alanine = D-alanine.UniRule annotationSAAS annotation

    Cofactori

    Pyridoxal phosphate.UniRule annotationSAAS annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei41 – 411Proton acceptor; specific for D-alanineUniRule annotation
    Binding sitei41 – 411AcetateImported
    Binding sitei131 – 1311Chloride 2Imported
    Binding sitei138 – 1381Chloride 1Imported
    Binding sitei138 – 1381Chloride 2Imported
    Binding sitei138 – 1381SubstrateUniRule annotation
    Binding sitei216 – 2161Chloride 3; via amide nitrogenImported
    Active sitei270 – 2701Proton acceptor; specific for L-alanineUniRule annotation
    Binding sitei317 – 3171Acetate; via amide nitrogenImported
    Binding sitei317 – 3171Substrate; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    1. alanine racemase activity Source: UniProtKB-HAMAP
    2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. D-alanine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    IsomeraseUniRule annotationSAAS annotationImported

    Keywords - Ligandi

    Pyridoxal phosphateUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciBANT260799:GJAJ-260-MONOMER.
    BANT261594:GJ7F-268-MONOMER.
    UniPathwayiUPA00042; UER00497.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine racemaseUniRule annotation (EC:5.1.1.1UniRule annotation)
    Gene namesi
    Name:dal1Imported
    Ordered Locus Names:BA_0252Imported, BAS0238Imported, GBAA_0252Imported
    OrganismiBacillus anthracisImported
    Taxonomic identifieri1392 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
    ProteomesiUP000000427: Chromosome, UP000005639: Chromosome, UP000000594: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei41 – 411N6-(pyridoxal phosphate)lysineUniRule annotation

    Interactioni

    Protein-protein interaction databases

    STRINGi198094.BA_0252.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2VD8X-ray1.47A/B1-389[»]
    2VD9X-ray2.10A/B1-389[»]
    3HA1X-ray1.95A/B1-389[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ81VF6.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alanine racemase family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000031444.
    KOiK01775.
    OMAiRTEEWIA.
    OrthoDBiEOG6PP9NJ.

    Family and domain databases

    Gene3Di2.40.37.10. 1 hit.
    3.20.20.10. 1 hit.
    HAMAPiMF_01201. Ala_racemase.
    InterProiIPR000821. Ala_racemase.
    IPR009006. Ala_racemase/Decarboxylase_C.
    IPR011079. Ala_racemase_C.
    IPR001608. Ala_racemase_N.
    IPR020622. Ala_racemase_pyridoxalP-BS.
    IPR029066. PLP-binding_barrel.
    [Graphical view]
    PfamiPF00842. Ala_racemase_C. 1 hit.
    PF01168. Ala_racemase_N. 1 hit.
    [Graphical view]
    PRINTSiPR00992. ALARACEMASE.
    SMARTiSM01005. Ala_racemase_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF50621. SSF50621. 1 hit.
    SSF51419. SSF51419. 1 hit.
    TIGRFAMsiTIGR00492. alr. 1 hit.
    PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q81VF6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEAPFYRDT WVEVDLDAIY NNVTHIKEFI PSDVEIFAVV KGNAYGHDYV    50
    PVAKIALEAG ATRLAVAFLD EALVLRRAGI TAPILVLGPS PPRDINVAAE 100
    NDVALTVFQK EWVDEAIKLW DGSSTMKYHI NFDSGMGRIG IRERKELKGF 150
    LKSLEGAPFL ELEGVYTHFA TADEVETSYF DKQYNTFLEQ LSWLKEFGVD 200
    PKFVHTANSA ATLRFQGITF NAVRIGIAMY GLSPSVEIRP FLPFKLEPAL 250
    SLHTKVAHIK QVIKGDGISY NVTYRTKTEE WIATVAIGYA DGWLRRLQGF 300
    EVLVNGKRVP IVGRVTMDQF MIHLPCEVPL GTKVTLIGRQ GDEYISATEV 350
    AEYSGTINYE IITTISFRVP RIFIRNGKVV EVINYLNDI 389
    Length:389
    Mass (Da):43,662
    Last modified:June 1, 2003 - v1
    Checksum:i5B55B2C4920D6BA5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016879 Genomic DNA. Translation: AAP24291.1.
    AE017334 Genomic DNA. Translation: AAT29333.1.
    AE017225 Genomic DNA. Translation: AAT52574.1.
    RefSeqiNP_842805.1. NC_003997.3.
    YP_016858.1. NC_007530.2.
    YP_026523.1. NC_005945.1.

    Genome annotation databases

    EnsemblBacteriaiAAP24291; AAP24291; BA_0252.
    AAT29333; AAT29333; GBAA_0252.
    AAT52574; AAT52574; BAS0238.
    GeneIDi1087014.
    2819200.
    2848190.
    KEGGiban:BA_0252.
    bar:GBAA_0252.
    bat:BAS0238.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016879 Genomic DNA. Translation: AAP24291.1 .
    AE017334 Genomic DNA. Translation: AAT29333.1 .
    AE017225 Genomic DNA. Translation: AAT52574.1 .
    RefSeqi NP_842805.1. NC_003997.3.
    YP_016858.1. NC_007530.2.
    YP_026523.1. NC_005945.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2VD8 X-ray 1.47 A/B 1-389 [» ]
    2VD9 X-ray 2.10 A/B 1-389 [» ]
    3HA1 X-ray 1.95 A/B 1-389 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198094.BA_0252.

    Protocols and materials databases

    DNASUi 1087014.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP24291 ; AAP24291 ; BA_0252 .
    AAT29333 ; AAT29333 ; GBAA_0252 .
    AAT52574 ; AAT52574 ; BAS0238 .
    GeneIDi 1087014.
    2819200.
    2848190.
    KEGGi ban:BA_0252.
    bar:GBAA_0252.
    bat:BAS0238.

    Phylogenomic databases

    HOGENOMi HOG000031444.
    KOi K01775.
    OMAi RTEEWIA.
    OrthoDBi EOG6PP9NJ.

    Enzyme and pathway databases

    UniPathwayi UPA00042 ; UER00497 .
    BioCyci BANT260799:GJAJ-260-MONOMER.
    BANT261594:GJ7F-268-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q81VF6.

    Family and domain databases

    Gene3Di 2.40.37.10. 1 hit.
    3.20.20.10. 1 hit.
    HAMAPi MF_01201. Ala_racemase.
    InterProi IPR000821. Ala_racemase.
    IPR009006. Ala_racemase/Decarboxylase_C.
    IPR011079. Ala_racemase_C.
    IPR001608. Ala_racemase_N.
    IPR020622. Ala_racemase_pyridoxalP-BS.
    IPR029066. PLP-binding_barrel.
    [Graphical view ]
    Pfami PF00842. Ala_racemase_C. 1 hit.
    PF01168. Ala_racemase_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00992. ALARACEMASE.
    SMARTi SM01005. Ala_racemase_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50621. SSF50621. 1 hit.
    SSF51419. SSF51419. 1 hit.
    TIGRFAMsi TIGR00492. alr. 1 hit.
    PROSITEi PS00395. ALANINE_RACEMASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
      Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
      , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
      Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AmesImported and Ames ancestor.
    2. "Complete genome sequence of Bacillus anthracis Sterne."
      Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
      Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SterneImported.
    3. "Structures of an alanine racemase from Bacillus anthracis (BA0252) in the presence and absence of (R)-1-aminoethylphosphonic acid (L-Ala-P)."
      Au K., Ren J., Walter T.S., Harlos K., Nettleship J.E., Owens R.J., Stuart D.I., Esnouf R.M.
      Acta Crystallogr. F 64:327-333(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.47 ANGSTROMS).
    4. "Biochemical and structural characterization of alanine racemase from Bacillus anthracis (Ames)."
      Counago R.M., Davlieva M., Strych U., Hill R.E., Krause K.L.
      BMC Struct. Biol. 9:53-53(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ames / isolate PortonImported, Ames ancestorImported, Ames AncestorImported and Sterne.
    6. Joardar V., Shrivastava S., Brinkac L.M., Harkins D.M., Durkin A.S., Sutton G.
      Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: AmesImported.

    Entry informationi

    Entry nameiQ81VF6_BACAN
    AccessioniPrimary (citable) accession number: Q81VF6
    Secondary accession number(s): E9R9C7
    , E9R9C8, Q6I4F7, Q6KY59
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 1, 2003
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3