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Protein

Alanine racemase

Gene

dal1

Organism
Bacillus anthracis
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.UniRule annotation

Catalytic activityi

L-alanine = D-alanine.UniRule annotationSAAS annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei41 – 411Proton acceptor; specific for D-alanineUniRule annotation
Binding sitei41 – 411AcetateCombined sources
Binding sitei131 – 1311Chloride 2Combined sources
Binding sitei138 – 1381Chloride 1Combined sources
Binding sitei138 – 1381Chloride 2Combined sources
Binding sitei138 – 1381SubstrateUniRule annotation
Binding sitei216 – 2161Chloride 3; via amide nitrogenCombined sources
Active sitei270 – 2701Proton acceptor; specific for L-alanineUniRule annotation
Binding sitei317 – 3171Acetate; via amide nitrogenCombined sources
Binding sitei317 – 3171Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. alanine racemase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. D-alanine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

IsomeraseUniRule annotationSAAS annotation

Keywords - Ligandi

Pyridoxal phosphateUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciBANT260799:GJAJ-260-MONOMER.
BANT261594:GJ7F-268-MONOMER.
UniPathwayiUPA00042; UER00497.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemaseUniRule annotation (EC:5.1.1.1UniRule annotation)
Gene namesi
Name:dal1Imported
Synonyms:alrImported, dal-1Imported
Ordered Locus Names:BA_0252Imported, BAS0238Imported, GBAA_0252Imported
ORF Names:BACvac02_0282Imported, BAHan_0286Imported, DH23_0240Imported, DJ42_5167Imported, DJ43_5593Imported, DJ44_4088Imported, DJ45_38Imported, DJ48_3980Imported, DJ49_5271Imported, HYU01_01340Imported, NX97_12960Imported, OY21_22935Imported, OY22_03720Imported, OY23_12880Imported, OY24_22890Imported, OY25_22375Imported, OY26_00070Imported, OY27_18790Imported, OY28_16845Imported, OY29_27495Imported, OY30_20995Imported, OY32_04720Imported, OY34_17795Imported, OY35_07420Imported, OY36_28325Imported, OY37_06675Imported, OY38_03380Imported, OY39_27895Imported, OY40_29060Imported
OrganismiBacillus anthracisImported
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000000427: Chromosome, UP000000594: Chromosome, UP000005639: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei41 – 411N6-(pyridoxal phosphate)lysineUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi198094.BA_0252.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VD8X-ray1.47A/B1-389[»]
2VD9X-ray2.10A/B1-389[»]
3HA1X-ray1.95A/B1-389[»]
ProteinModelPortaliQ81VF6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ81VF6.

Family & Domainsi

Sequence similaritiesi

Belongs to the alanine racemase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000031444.
KOiK01775.
OMAiTLMAHFA.
OrthoDBiEOG6PP9NJ.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81VF6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEEAPFYRDT WVEVDLDAIY NNVTHIKEFI PSDVEIFAVV KGNAYGHDYV
60 70 80 90 100
PVAKIALEAG ATRLAVAFLD EALVLRRAGI TAPILVLGPS PPRDINVAAE
110 120 130 140 150
NDVALTVFQK EWVDEAIKLW DGSSTMKYHI NFDSGMGRIG IRERKELKGF
160 170 180 190 200
LKSLEGAPFL ELEGVYTHFA TADEVETSYF DKQYNTFLEQ LSWLKEFGVD
210 220 230 240 250
PKFVHTANSA ATLRFQGITF NAVRIGIAMY GLSPSVEIRP FLPFKLEPAL
260 270 280 290 300
SLHTKVAHIK QVIKGDGISY NVTYRTKTEE WIATVAIGYA DGWLRRLQGF
310 320 330 340 350
EVLVNGKRVP IVGRVTMDQF MIHLPCEVPL GTKVTLIGRQ GDEYISATEV
360 370 380
AEYSGTINYE IITTISFRVP RIFIRNGKVV EVINYLNDI
Length:389
Mass (Da):43,662
Last modified:June 1, 2003 - v1
Checksum:i5B55B2C4920D6BA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP24291.1.
AE017334 Genomic DNA. Translation: AAT29333.1.
AE017225 Genomic DNA. Translation: AAT52574.1.
CP008846 Genomic DNA. Translation: AIF54733.1.
CP007660 Genomic DNA. Translation: AIK06366.1.
CP007618 Genomic DNA. Translation: AIK31287.1.
CP008752 Genomic DNA. Translation: AIK49720.1.
CP007704 Genomic DNA. Translation: AIK59672.1.
CP008853 Genomic DNA. Translation: AIM04221.1.
CP008854 Genomic DNA. Translation: AIM09607.1.
JNOD01000008 Genomic DNA. Translation: KFJ83507.1.
JMPV01000005 Genomic DNA. Translation: KFL76891.1.
JMPU01000008 Genomic DNA. Translation: KFL80532.1.
JTAQ01000031 Genomic DNA. Translation: KGZ43031.1.
JTAS01000049 Genomic DNA. Translation: KGZ52702.1.
JTAR01000017 Genomic DNA. Translation: KGZ54716.1.
JSZQ01000046 Genomic DNA. Translation: KGZ60962.1.
JSZR01000029 Genomic DNA. Translation: KGZ64641.1.
JSZP01000039 Genomic DNA. Translation: KGZ65596.1.
JSZT01000044 Genomic DNA. Translation: KGZ76660.1.
JSZU01000032 Genomic DNA. Translation: KGZ80222.1.
JSZS01000001 Genomic DNA. Translation: KGZ90246.1.
JSZV01000041 Genomic DNA. Translation: KGZ90970.1.
JSZW01000041 Genomic DNA. Translation: KGZ96979.1.
JSZY01000023 Genomic DNA. Translation: KHA06001.1.
JTAA01000036 Genomic DNA. Translation: KHA11862.1.
JTAC01000089 Genomic DNA. Translation: KHA11995.1.
JTAB01000033 Genomic DNA. Translation: KHA16024.1.
JTAD01000011 Genomic DNA. Translation: KHA31775.1.
JTAF01000052 Genomic DNA. Translation: KHA31812.1.
JTAE01000012 Genomic DNA. Translation: KHA36193.1.
JTAG01000063 Genomic DNA. Translation: KHA39121.1.
RefSeqiNP_842805.1. NC_003997.3.
YP_016858.1. NC_007530.2.
YP_026523.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP24291; AAP24291; BA_0252.
AAT29333; AAT29333; GBAA_0252.
AAT52574; AAT52574; BAS0238.
GeneIDi1087014.
2819200.
2848190.
KEGGiban:BA_0252.
bar:GBAA_0252.
bat:BAS0238.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP24291.1.
AE017334 Genomic DNA. Translation: AAT29333.1.
AE017225 Genomic DNA. Translation: AAT52574.1.
CP008846 Genomic DNA. Translation: AIF54733.1.
CP007660 Genomic DNA. Translation: AIK06366.1.
CP007618 Genomic DNA. Translation: AIK31287.1.
CP008752 Genomic DNA. Translation: AIK49720.1.
CP007704 Genomic DNA. Translation: AIK59672.1.
CP008853 Genomic DNA. Translation: AIM04221.1.
CP008854 Genomic DNA. Translation: AIM09607.1.
JNOD01000008 Genomic DNA. Translation: KFJ83507.1.
JMPV01000005 Genomic DNA. Translation: KFL76891.1.
JMPU01000008 Genomic DNA. Translation: KFL80532.1.
JTAQ01000031 Genomic DNA. Translation: KGZ43031.1.
JTAS01000049 Genomic DNA. Translation: KGZ52702.1.
JTAR01000017 Genomic DNA. Translation: KGZ54716.1.
JSZQ01000046 Genomic DNA. Translation: KGZ60962.1.
JSZR01000029 Genomic DNA. Translation: KGZ64641.1.
JSZP01000039 Genomic DNA. Translation: KGZ65596.1.
JSZT01000044 Genomic DNA. Translation: KGZ76660.1.
JSZU01000032 Genomic DNA. Translation: KGZ80222.1.
JSZS01000001 Genomic DNA. Translation: KGZ90246.1.
JSZV01000041 Genomic DNA. Translation: KGZ90970.1.
JSZW01000041 Genomic DNA. Translation: KGZ96979.1.
JSZY01000023 Genomic DNA. Translation: KHA06001.1.
JTAA01000036 Genomic DNA. Translation: KHA11862.1.
JTAC01000089 Genomic DNA. Translation: KHA11995.1.
JTAB01000033 Genomic DNA. Translation: KHA16024.1.
JTAD01000011 Genomic DNA. Translation: KHA31775.1.
JTAF01000052 Genomic DNA. Translation: KHA31812.1.
JTAE01000012 Genomic DNA. Translation: KHA36193.1.
JTAG01000063 Genomic DNA. Translation: KHA39121.1.
RefSeqiNP_842805.1. NC_003997.3.
YP_016858.1. NC_007530.2.
YP_026523.1. NC_005945.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VD8X-ray1.47A/B1-389[»]
2VD9X-ray2.10A/B1-389[»]
3HA1X-ray1.95A/B1-389[»]
ProteinModelPortaliQ81VF6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198094.BA_0252.

Protocols and materials databases

DNASUi1087014.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP24291; AAP24291; BA_0252.
AAT29333; AAT29333; GBAA_0252.
AAT52574; AAT52574; BAS0238.
GeneIDi1087014.
2819200.
2848190.
KEGGiban:BA_0252.
bar:GBAA_0252.
bat:BAS0238.

Phylogenomic databases

HOGENOMiHOG000031444.
KOiK01775.
OMAiTLMAHFA.
OrthoDBiEOG6PP9NJ.

Enzyme and pathway databases

UniPathwayiUPA00042; UER00497.
BioCyciBANT260799:GJAJ-260-MONOMER.
BANT261594:GJ7F-268-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ81VF6.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
    Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
    , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
    Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AmesImported and Ames / isolate PortonImported.
  2. "Complete genome sequence of Bacillus anthracis Sterne."
    Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SterneImported.
  3. "Structures of an alanine racemase from Bacillus anthracis (BA0252) in the presence and absence of (R)-1-aminoethylphosphonic acid (L-Ala-P)."
    Au K., Ren J., Walter T.S., Harlos K., Nettleship J.E., Owens R.J., Stuart D.I., Esnouf R.M.
    Acta Crystallogr. F 64:327-333(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.47 ANGSTROMS).
  4. "Biochemical and structural characterization of alanine racemase from Bacillus anthracis (Ames)."
    Counago R.M., Davlieva M., Strych U., Hill R.E., Krause K.L.
    BMC Struct. Biol. 9:53-53(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames ancestorImported and Ames AncestorImported.
  6. Joardar V., Shrivastava S., Brinkac L.M., Harkins D.M., Durkin A.S., Sutton G.
    Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: AmesImported.
  7. Tong Y.G., Huang Y., An X.P., Li S.S., Mi Z.Q., Pei G.Q., Zhang Z.Y., Fan H.
    Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Cvac02Imported and HanImported.
  8. "Complete Genome Sequence of Bacillus anthracis HYU01, Isolated from Soil Samples in the Korean Peninsula."
    Kim S.K., Chung W.H., Kim S.H., Jung K.H., Kim N., Chai Y.G.
    Genome Announc. 2:e00769-14(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: HYU01Imported.
  9. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 2000031021Imported, A.Br.003Imported, Smith 1013Imported and Zimbabwe 89Imported.
  10. "Draft Genome Sequence of Bacillus anthracis STI."
    Okinaka R.T., Challacombe J.F., Drees K., Birdsell D.N., Janke N., Naumann A., Seymour M., O'Neill H.H., Schupp J., Sahl J., Foster J.T., Pearson T., Keim P.
    Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: STI-1Imported.
  11. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: BFVImported.
  12. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Delta SterneImported.
  13. "Centers for Disease Control and Prevention Bacillus anthracis whole genome sequencing."
    Hoffmaster A.R., Marston C.K., Gee J., Tiller R., Beesley C., Bell M., Elrod M., France M., Sammons S., Rodriguez-R L.M., Weigand M.R., Konstantinidis K.T.
    Submitted (NOV-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 2000031027Imported.
  14. "Centers for Disease Control and Prevention Bacillus anthracis whole genome sequencing."
    Hoffmaster A.R., Marston C.K., Gee J., Tiller R., Beesley C.A., Bell M., Elrod M., France M., Sammons S., Rodriguez-R L.M., Weigand M.R., Konstantinidis K.T.
    Submitted (NOV-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 2000031006Imported, 2000031023Imported, 2000031031Imported, 2000031038Imported, 2000031039Imported, 2000031052Imported, 2000031075Imported, 2000031709Imported, 2000031765Imported, 2000032819Imported, 2000032879Imported, 2000032951Imported, 2000032967Imported, 2000032968Imported, 2000032975Imported, 2000032979Imported, 2000032989Imported and 2002013094Imported.

Entry informationi

Entry nameiQ81VF6_BACAN
AccessioniPrimary (citable) accession number: Q81VF6
Secondary accession number(s): E9R9C7
, E9R9C8, Q6I4F7, Q6KY59
Entry historyi
Integrated into UniProtKB/TrEMBL: June 1, 2003
Last sequence update: June 1, 2003
Last modified: February 4, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.