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Protein

ATP-dependent helicase/nuclease subunit A

Gene

addA

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddA nuclease domain is required for chi fragment generation; this subunit has the helicase and 3' -> 5' nuclease activities.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi33 – 40ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

  • DNA metabolic process Source: TIGR
  • double-strand break repair via homologous recombination Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciANTHRA:ADDA-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent helicase/nuclease subunit AUniRule annotation (EC:3.1.-.-UniRule annotation, EC:3.6.4.12UniRule annotation)
Alternative name(s):
ATP-dependent helicase/nuclease AddAUniRule annotation
Gene namesi
Name:addAUniRule annotation
Ordered Locus Names:BA_1142, GBAA_1142, BAS1061
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003792311 – 1241ATP-dependent helicase/nuclease subunit AAdd BLAST1241

Proteomic databases

PRIDEiQ81TW1.

Interactioni

Subunit structurei

Heterodimer of AddA and AddB/RexB.UniRule annotation

Protein-protein interaction databases

IntActiQ81TW1. 13 interactors.
STRINGi198094.BA_1142.

Structurei

3D structure databases

ProteinModelPortaliQ81TW1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 485UvrD-like helicase ATP-bindingUniRule annotationAdd BLAST474
Domaini505 – 805UvrD-like helicase C-terminalUniRule annotationAdd BLAST301

Sequence similaritiesi

Belongs to the helicase family. AddA subfamily.UniRule annotation
Contains 1 uvrD-like helicase ATP-binding domain.UniRule annotation
Contains 1 uvrD-like helicase C-terminal domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R2I. Bacteria.
COG1074. LUCA.
HOGENOMiHOG000015621.
KOiK16898.
OMAiENAFEFS.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
3.90.320.10. 1 hit.
HAMAPiMF_01451. AddA. 1 hit.
InterProiIPR014152. DNA_helicase_suAddA.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR011604. Exonuc_phg/RecB_C.
IPR027417. P-loop_NTPase.
IPR011335. Restrct_endonuc-II-like.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 4 hits.
PfamiPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
SSF52980. SSF52980. 1 hit.
TIGRFAMsiTIGR02785. addA_Gpos. 1 hit.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81TW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMENWPKKPE GSQWTDDQWK AVVATGRDIL VAAAAGSGKT AVLVERIIKK
60 70 80 90 100
IINEENPVDV DRLLVVTFTN AAAQEMKNRI GEALEKVLID EPGSQHVRKQ
110 120 130 140 150
LSLLNKASIS TIHSFCLQVI RGYYYMLDVD PRFRIANQTE NELLKEEVLD
160 170 180 190 200
DILEEEYGIE DNTIFFELVD RYTSDRSDDD LQRMILALHT ESRAHPNPEK
210 220 230 240 250
WLDKLVEAYD VEGKTIEDLV YASYLLEDVK FQLETAEQHI RKATELAMLP
260 270 280 290 300
DGPAPRIETL QADLALLGTL SAAARESWTS VYEAMQNVSW QTLKRIKKSD
310 320 330 340 350
YNEDIVKQVD SLRNKAKDEV KKLQEELFSR RPESFLRDFQ DMHPVLEKLV
360 370 380 390 400
QLVKVFTERF QAMKRDKGMV DFTDLEHFCL QILSEQSEDG EMKPSAVALQ
410 420 430 440 450
YRNKFAEVLV DEYQDTNFVQ ESIIKFVTKD SESEGNLFMV GDVKQSIYRF
460 470 480 490 500
RLAEPGLFLG KYKRFTQEGL GGGMKIDLAK NFRSRHEVLA GTNFIFKQIM
510 520 530 540 550
GEEVGEIDYD ADAELKLGAS YPEGEDVAAE LLCIQQTEEE VIDGEEGAEV
560 570 580 590 600
EKAQLEARLM AQRIKAMVDS GYEVYDRKTD SMRPVKYRDF VILLRSMPWA
610 620 630 640 650
PQIMEELKLQ GIPVYADLAT GYFEATEVNI MMNVFRVIDN PMQDIPLAAV
660 670 680 690 700
LRSPIVGLND EELATLRAHG KKGSFYEVMS SFLKGAPLEE EKELHDKLEW
710 720 730 740 750
FYNLLQGWRE FARQQSLSDL IWKVYGETGY YDFVGGLPAG KQRQANLRVL
760 770 780 790 800
YDRARQYEAT SFRGLFRFLR FIERILERGD DMGTARALGE QEDVVRIMTI
810 820 830 840 850
HKSKGLEFPV VFVAGLGRRF NTQDLMKRFL LHKDFGFGSQ FIDPRKRIKY
860 870 880 890 900
TTLSQLAIKR KMKMELIAEE MRVLYVALTR AKEKLILIGT VKDATKEMEK
910 920 930 940 950
WLDAREHSEW LLPDHVRAGA SCYLDWIAPS LYRHRDSEML LELGQGSIPD
960 970 980 990 1000
EIYGYDTSWK VEVVDGNTLL APEPVQEEKQ ELLEALREKK AVPLESERKE
1010 1020 1030 1040 1050
EVYDRLMWKY GYGEATSHRA KQSVTEIKRN YQSEEGSDNA FIKKLRAPIQ
1060 1070 1080 1090 1100
TRPRFMEKKG LTYAERGTAV HAVMQHVDLK KPITVEILQE QIAGMVNKEL
1110 1120 1130 1140 1150
LTFEQAEEIA IEKVISFFDS DLGKRVLAAK SVEREVPFTM MLAAEEAYQD
1160 1170 1180 1190 1200
WQGESGESIL VQGVIDCMIE EEDGITLIDF KTDTIEGKFP GGFEQAKPIL
1210 1220 1230 1240
ETRYKVQLSL YAKALEKSLQ HPVKEKCLYF FDGNHVIKVE E
Length:1,241
Mass (Da):142,623
Last modified:June 1, 2003 - v1
Checksum:iECD5097DCFE5D2C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP25112.1.
AE017334 Genomic DNA. Translation: AAT30235.1.
AE017225 Genomic DNA. Translation: AAT53384.1.
RefSeqiNP_843626.1. NC_003997.3.
WP_000970458.1. NZ_LHUN01000008.1.
YP_027333.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP25112; AAP25112; BA_1142.
AAT30235; AAT30235; GBAA_1142.
AAT53384; AAT53384; BAS1061.
GeneIDi1089102.
2848175.
KEGGiban:BA_1142.
bar:GBAA_1142.
bat:BAS1061.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP25112.1.
AE017334 Genomic DNA. Translation: AAT30235.1.
AE017225 Genomic DNA. Translation: AAT53384.1.
RefSeqiNP_843626.1. NC_003997.3.
WP_000970458.1. NZ_LHUN01000008.1.
YP_027333.1. NC_005945.1.

3D structure databases

ProteinModelPortaliQ81TW1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ81TW1. 13 interactors.
STRINGi198094.BA_1142.

Proteomic databases

PRIDEiQ81TW1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP25112; AAP25112; BA_1142.
AAT30235; AAT30235; GBAA_1142.
AAT53384; AAT53384; BAS1061.
GeneIDi1089102.
2848175.
KEGGiban:BA_1142.
bar:GBAA_1142.
bat:BAS1061.

Phylogenomic databases

eggNOGiENOG4107R2I. Bacteria.
COG1074. LUCA.
HOGENOMiHOG000015621.
KOiK16898.
OMAiENAFEFS.

Enzyme and pathway databases

BioCyciANTHRA:ADDA-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
3.90.320.10. 1 hit.
HAMAPiMF_01451. AddA. 1 hit.
InterProiIPR014152. DNA_helicase_suAddA.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR011604. Exonuc_phg/RecB_C.
IPR027417. P-loop_NTPase.
IPR011335. Restrct_endonuc-II-like.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 4 hits.
PfamiPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
SSF52980. SSF52980. 1 hit.
TIGRFAMsiTIGR02785. addA_Gpos. 1 hit.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADDA_BACAN
AccessioniPrimary (citable) accession number: Q81TW1
Secondary accession number(s): Q6I247, Q6KVY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.