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Protein

ATP-dependent helicase/nuclease subunit A

Gene

addA

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddA nuclease domain is required for chi fragment generation; this subunit has the helicase and 3' -> 5' nuclease activities.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi33 – 40ATPUniRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent DNA helicase activity Source: InterPro
  • DNA binding Source: UniProtKB-KW
  • exonuclease activity Source: TIGR

GO - Biological processi

  • DNA metabolic process Source: TIGR
  • double-strand break repair Source: InterPro

Keywordsi

Molecular functionDNA-binding, Exonuclease, Helicase, Hydrolase, Nuclease
Biological processDNA damage, DNA repair
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent helicase/nuclease subunit AUniRule annotation (EC:3.1.-.-UniRule annotation, EC:3.6.4.12UniRule annotation)
Alternative name(s):
ATP-dependent helicase/nuclease AddAUniRule annotation
Gene namesi
Name:addAUniRule annotation
Ordered Locus Names:BA_1142, GBAA_1142, BAS1061
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003792311 – 1241ATP-dependent helicase/nuclease subunit AAdd BLAST1241

Proteomic databases

PRIDEiQ81TW1

Interactioni

Subunit structurei

Heterodimer of AddA and AddB/RexB.UniRule annotation

Protein-protein interaction databases

IntActiQ81TW1, 13 interactors
STRINGi260799.BAS1061

Structurei

3D structure databases

ProteinModelPortaliQ81TW1
SMRiQ81TW1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 485UvrD-like helicase ATP-bindingUniRule annotationAdd BLAST474
Domaini505 – 805UvrD-like helicase C-terminalUniRule annotationAdd BLAST301

Sequence similaritiesi

Belongs to the helicase family. AddA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R2I Bacteria
COG1074 LUCA
HOGENOMiHOG000015621
KOiK16898
OMAiKQSIYRW

Family and domain databases

Gene3Di3.90.320.10, 1 hit
HAMAPiMF_01451 AddA, 1 hit
InterProiView protein in InterPro
IPR014152 DNA_helicase_suAddA
IPR014017 DNA_helicase_UvrD-like_C
IPR000212 DNA_helicase_UvrD/REP
IPR011604 Exonuc_phg/RecB_C
IPR027417 P-loop_NTPase
IPR038726 PDDEXK_AddAB-type
IPR011335 Restrct_endonuc-II-like
IPR014016 UvrD-like_ATP-bd
IPR034739 UvrD/AddA_N
PANTHERiPTHR11070 PTHR11070, 1 hit
PTHR11070:SF14 PTHR11070:SF14, 1 hit
PfamiView protein in Pfam
PF12705 PDDEXK_1, 1 hit
PF00580 UvrD-helicase, 1 hit
PF13361 UvrD_C, 1 hit
SUPFAMiSSF52540 SSF52540, 4 hits
SSF52980 SSF52980, 1 hit
TIGRFAMsiTIGR02785 addA_Gpos, 1 hit
PROSITEiView protein in PROSITE
PS51198 UVRD_HELICASE_ATP_BIND, 1 hit
PS51217 UVRD_HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

Q81TW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMENWPKKPE GSQWTDDQWK AVVATGRDIL VAAAAGSGKT AVLVERIIKK
60 70 80 90 100
IINEENPVDV DRLLVVTFTN AAAQEMKNRI GEALEKVLID EPGSQHVRKQ
110 120 130 140 150
LSLLNKASIS TIHSFCLQVI RGYYYMLDVD PRFRIANQTE NELLKEEVLD
160 170 180 190 200
DILEEEYGIE DNTIFFELVD RYTSDRSDDD LQRMILALHT ESRAHPNPEK
210 220 230 240 250
WLDKLVEAYD VEGKTIEDLV YASYLLEDVK FQLETAEQHI RKATELAMLP
260 270 280 290 300
DGPAPRIETL QADLALLGTL SAAARESWTS VYEAMQNVSW QTLKRIKKSD
310 320 330 340 350
YNEDIVKQVD SLRNKAKDEV KKLQEELFSR RPESFLRDFQ DMHPVLEKLV
360 370 380 390 400
QLVKVFTERF QAMKRDKGMV DFTDLEHFCL QILSEQSEDG EMKPSAVALQ
410 420 430 440 450
YRNKFAEVLV DEYQDTNFVQ ESIIKFVTKD SESEGNLFMV GDVKQSIYRF
460 470 480 490 500
RLAEPGLFLG KYKRFTQEGL GGGMKIDLAK NFRSRHEVLA GTNFIFKQIM
510 520 530 540 550
GEEVGEIDYD ADAELKLGAS YPEGEDVAAE LLCIQQTEEE VIDGEEGAEV
560 570 580 590 600
EKAQLEARLM AQRIKAMVDS GYEVYDRKTD SMRPVKYRDF VILLRSMPWA
610 620 630 640 650
PQIMEELKLQ GIPVYADLAT GYFEATEVNI MMNVFRVIDN PMQDIPLAAV
660 670 680 690 700
LRSPIVGLND EELATLRAHG KKGSFYEVMS SFLKGAPLEE EKELHDKLEW
710 720 730 740 750
FYNLLQGWRE FARQQSLSDL IWKVYGETGY YDFVGGLPAG KQRQANLRVL
760 770 780 790 800
YDRARQYEAT SFRGLFRFLR FIERILERGD DMGTARALGE QEDVVRIMTI
810 820 830 840 850
HKSKGLEFPV VFVAGLGRRF NTQDLMKRFL LHKDFGFGSQ FIDPRKRIKY
860 870 880 890 900
TTLSQLAIKR KMKMELIAEE MRVLYVALTR AKEKLILIGT VKDATKEMEK
910 920 930 940 950
WLDAREHSEW LLPDHVRAGA SCYLDWIAPS LYRHRDSEML LELGQGSIPD
960 970 980 990 1000
EIYGYDTSWK VEVVDGNTLL APEPVQEEKQ ELLEALREKK AVPLESERKE
1010 1020 1030 1040 1050
EVYDRLMWKY GYGEATSHRA KQSVTEIKRN YQSEEGSDNA FIKKLRAPIQ
1060 1070 1080 1090 1100
TRPRFMEKKG LTYAERGTAV HAVMQHVDLK KPITVEILQE QIAGMVNKEL
1110 1120 1130 1140 1150
LTFEQAEEIA IEKVISFFDS DLGKRVLAAK SVEREVPFTM MLAAEEAYQD
1160 1170 1180 1190 1200
WQGESGESIL VQGVIDCMIE EEDGITLIDF KTDTIEGKFP GGFEQAKPIL
1210 1220 1230 1240
ETRYKVQLSL YAKALEKSLQ HPVKEKCLYF FDGNHVIKVE E
Length:1,241
Mass (Da):142,623
Last modified:June 1, 2003 - v1
Checksum:iECD5097DCFE5D2C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA Translation: AAP25112.1
AE017334 Genomic DNA Translation: AAT30235.1
AE017225 Genomic DNA Translation: AAT53384.1
RefSeqiNP_843626.1, NC_003997.3
WP_000970458.1, NZ_PPES01000003.1
YP_027333.1, NC_005945.1

Genome annotation databases

EnsemblBacteriaiAAP25112; AAP25112; BA_1142
AAT30235; AAT30235; GBAA_1142
AAT53384; AAT53384; BAS1061
GeneIDi1089102
2848175
KEGGiban:BA_1142
bar:GBAA_1142
bat:BAS1061
PATRICifig|198094.11.peg.1123

Similar proteinsi

Entry informationi

Entry nameiADDA_BACAN
AccessioniPrimary (citable) accession number: Q81TW1
Secondary accession number(s): Q6I247, Q6KVY3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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