Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NAD kinase 1

Gene

nadK1

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei45 – 451Proton acceptorUniRule annotation
Binding sitei148 – 1481NADUniRule annotation
Binding sitei150 – 1501NADUniRule annotation
Binding sitei185 – 1851NAD; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi45 – 462NADUniRule annotation
Nucleotide bindingi122 – 1232NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciBANT260799:GJAJ-1196-MONOMER.
BANT261594:GJ7F-1252-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinase 1UniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinase 1UniRule annotation
Gene namesi
Name:nadK1UniRule annotation
Ordered Locus Names:BA_1213, GBAA_1213, BAS1120
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000000427: Chromosome, UP000000594: Chromosome, UP000005639: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 265265NAD kinase 1PRO_0000120591Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi198094.BA_1213.

Structurei

3D structure databases

ProteinModelPortaliQ81TQ3.
SMRiQ81TQ3. Positions 1-262.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000275803.
KOiK00858.
OMAiHRYENQV.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

Q81TQ3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFTIMSKGD QSSDTLASTM KEYLLDFGFI MDEQEPDIVI SVGGDGTLLY
60 70 80 90 100
AFHRYYNRLD ETAFVGVHTG HLGFYADWLP TEVEKLVIAI AKTPFQVVEY
110 120 130 140 150
PLLEVIIRYM NGSKESQYLA MNEATVKSAE GTLVTEVEIR GEYFETFRGD
160 170 180 190 200
GLCISTPSGS TAYNKALGGA IIHPSIEAIQ IAEMASINNR VFRTVGSPLV
210 220 230 240 250
LPKHHTCVLK PTAGMNLQIT VDHLTMVHQD VKSIQYRVAN EKVRFVRFRP
260
FPFWKRVRDS FVADK
Length:265
Mass (Da):29,828
Last modified:June 1, 2003 - v1
Checksum:i1ABE86E26E84AB94
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP25174.1.
AE017334 Genomic DNA. Translation: AAT30299.1.
AE017225 Genomic DNA. Translation: AAT53443.1.
RefSeqiNP_843688.1. NC_003997.3.
YP_017824.1. NC_007530.2.
YP_027392.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP25174; AAP25174; BA_1213.
AAT30299; AAT30299; GBAA_1213.
AAT53443; AAT53443; BAS1120.
GeneIDi1088735.
2815246.
2848724.
KEGGiban:BA_1213.
bar:GBAA_1213.
bat:BAS1120.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP25174.1.
AE017334 Genomic DNA. Translation: AAT30299.1.
AE017225 Genomic DNA. Translation: AAT53443.1.
RefSeqiNP_843688.1. NC_003997.3.
YP_017824.1. NC_007530.2.
YP_027392.1. NC_005945.1.

3D structure databases

ProteinModelPortaliQ81TQ3.
SMRiQ81TQ3. Positions 1-262.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198094.BA_1213.

Protocols and materials databases

DNASUi1088735.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP25174; AAP25174; BA_1213.
AAT30299; AAT30299; GBAA_1213.
AAT53443; AAT53443; BAS1120.
GeneIDi1088735.
2815246.
2848724.
KEGGiban:BA_1213.
bar:GBAA_1213.
bat:BAS1120.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000275803.
KOiK00858.
OMAiHRYENQV.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciBANT260799:GJAJ-1196-MONOMER.
BANT261594:GJ7F-1252-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
    Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
    , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
    Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames / isolate Porton.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames ancestor.
  3. "Complete genome sequence of Bacillus anthracis Sterne."
    Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sterne.

Entry informationi

Entry nameiNADK1_BACAN
AccessioniPrimary (citable) accession number: Q81TQ3
Secondary accession number(s): Q6I1Y8, Q6KVS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: June 1, 2003
Last modified: January 7, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.