Q81TQ0 (PRPE_BACAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical]
EC=3.6.1.17

Alternative name(s):
Ap4A hydrolase
Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase
Short name=Diadenosine tetraphosphatase

Gene names

Name:	prpE
Ordered Locus Names:	BA_1217, GBAA_1217, BAS1124

Organism

Bacillus anthracis

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Asymmetrically hydrolyzes Ap4p to yield AMP and ATP By similarity. HAMAP MF_01443
Catalytic activity	P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP. HAMAP MF_01443
Cofactor	Nickel By similarity. HAMAP MF_01443
Sequence similarities	Belongs to the prpE family.

Ontologies

Keywords
Ligand	GTP-binding Nickel Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity Inferred from electronic annotation. Source: EC nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] HAMAP MF_01443		PRO_0000297695

Sequences

	Sequence		Length	Mass (Da)
	Q81TQ0 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 6868D3223207DCA8	FASTA	246	28,269
	10 20 30 40 50 60 MKYDIIGDIH GCLQEFQNLT EKLGYNWSSG LPVHPDQRKL AFVGDITDRG PHSLRMIEIV 70 80 90 100 110 120 WELVIHKKVA YYAPGNHCNK LYRFFLGRNV TIAHGLETTV AEYEALPSHK QNMIKEKFIT 130 140 150 160 170 180 LYEQSPLYHI LDEKRLLVCH AGIRQDYIGR QDKKVQTFVL YGDITGEKHA DGSPVRRDWA 190 200 210 220 230 240 KEYKGTTWIV YGHTPVKEPR FVNHTVNIDT GAVFGGRLTA LRYPEMETVS VPSSLPFVPE KFRPIS

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP25177.1. AE017334 Genomic DNA. Translation: AAT30303.1. AE017225 Genomic DNA. Translation: AAT53447.1.
RefSeq	NP_843691.1. NC_003997.3. YP_017828.1. NC_007530.2. YP_027396.1. NC_005945.1.
3D structure databases
ProteinModelPortal	Q81TQ0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q81TQ0. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000009621; EBBACP00000009377; EBBACG00000009613. EBBACT00000018143; EBBACP00000017764; EBBACG00000018134. EBBACT00000023648; EBBACP00000023133; EBBACG00000023639.
GeneID	1087409. 2815189. 2849282.
GenomeReviews	Gene locus BA_1217 in contig AE016879_GR. Gene locus BAS1124 in contig AE017225_GR. Gene locus GBAA_1217 in contig AE017334_GR.
KEGG	ban:BA_1217. bar:GBAA_1217. bat:BAS1124.
TIGR	BA_1217. GBAA_1217.
Phylogenomic databases
GeneTree	EBGT00050000002430.
HOGENOM	HBG724457.
OMA	YVPGNHC.
ProtClustDB	PRK13625.
Enzyme and pathway databases
BioCyc	BANT260799:BAS1124-MONOMER. BANT261594:GBAA1217-MONOMER.
Family and domain databases
HAMAP	MF_01443. PrpE. [Tree]
InterPro	IPR023937. Bis(5'-nucleosyl)-tetraP_PrpE. IPR004843. Metallo_PEstase_dom. [Graphical view]
KO	K01090.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRPE_BACAN

Accession

Primary (citable) accession number: Q81TQ0
Secondary accession number(s): Q6I1Y4, Q6KVS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	June 1, 2003
Last modified:	November 16, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program