Q81TL7 (TRPA_BACAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan synthase alpha chain
EC=4.2.1.20

Gene names

Name:	trpA
Ordered Locus Names:	BA_1254, GBAA_1254, BAS1162

Organism

Bacillus anthracis [Reference proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate By similarity. HAMAP-Rule MF_00131
Catalytic activity	L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + glyceraldehyde 3-phosphate + H2O. HAMAP-Rule MF_00131
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5. HAMAP-Rule MF_00131
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Sequence similarities	Belongs to the TrpA family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	tryptophan synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Tryptophan synthase alpha chain HAMAP-Rule MF_00131		PRO_0000098732
Sites
	Active site	47	1	Proton acceptor By similarity
	Active site	58	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81TL7 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 78A7946B146004EF	FASTA	258	28,355
	10 20 30 40 50 60 MGVERIKAAF ENGKKAFIPY VMGGDGGLEI LKERIRFLDE AGASIVEIGI PFSDPVADGP 70 80 90 100 110 120 TIQRAGKRAL DSGVTVKGIF QALIEVRKEV QIPFVLMTYL NPVLAFGKER FIENCMEAGV 130 140 150 160 170 180 DGIIVPDLPY EEQDIIAPLL REANIALIPL VTVTSPIERI KKITSESEGF VYAVTVAGVT 190 200 210 220 230 240 GVRQNFKDEI HSYLEKVKSH THLPVVAGFG ISTKEHVEEM VTICDGVVVG SKVIELLENE 250 KREEICEFIQ ATKQKEEA

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"The complete genome sequence of Bacillus anthracis Ames 'Ancestor'." Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D., Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A. J. Bacteriol. 191:445-446(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP25212.1. AE017334 Genomic DNA. Translation: AAT30344.1. AE017225 Genomic DNA. Translation: AAT53484.1.
RefSeq	NP_843726.1. NC_003997.3. YP_017869.1. NC_007530.2. YP_027433.1. NC_005945.1.
3D structure databases
ProteinModelPortal	Q81TL7.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q81TL7. 1 interaction.
STRING	198094.BA_1254.
Protocols and materials databases
DNASU	1085329.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAP25212; AAP25212; BA_1254. AAT30344; AAT30344; GBAA_1254. AAT53484; AAT53484; BAS1162.
GeneID	1085329. 2814761. 2852220.
KEGG	ban:BA_1254. bar:GBAA_1254. bat:BAS1162.
Phylogenomic databases
eggNOG	COG0159.
HOGENOM	HOG000223816.
KO	K01695.
OMA	VFICPPN.
ProtClustDB	PRK13111.
Enzyme and pathway databases
BioCyc	BANT260799:GJAJ-1237-MONOMER. BANT261594:GJ7F-1291-MONOMER.
UniPathway	UPA00035; UER00044.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00131. Trp_synth_alpha.
InterPro	IPR013785. Aldolase_TIM. IPR011060. RibuloseP-bd_barrel. IPR018204. Trp_synthase_alpha_AS. IPR002028. Trp_synthase_suA. [Graphical view]
Pfam	PF00290. Trp_syntA. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00262. trpA. 1 hit.
PROSITE	PS00167. TRP_SYNTHASE_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPA_BACAN

Accession

Primary (citable) accession number: Q81TL7
Secondary accession number(s): Q6I1U7, Q6KVN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program