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Reviewed, UniProtKB/Swiss-Prot Q81TC4 (QUEF_BACAN)

Last modified February 9, 2010. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase
Gene names
Name: queF
Ordered Locus Names: BA_1362, GBAA_1362, BAS1260
OrganismBacillus anthracis [Complete proteome] [HAMAP]
Taxonomic identifier1392 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacillalesBacillaceaeBacillusBacillus cereus group

Protein attributes

Sequence length165 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00818

Catalytic activity

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00818

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00818

Subcellular location

Cytoplasm Probable HAMAP MF_00818.

Sequence similarities

Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 165165NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00818
PRO_0000162950

Sequences

Sequence LengthMass (Da)Tools
Q81TC4-1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: E08FBCCA7A7FFAE3

FASTA16519,528
        10         20         30         40         50         60 
MAGRLDEDLK DVTLLGNQNT KYLFEYSPEI LEVFDNNHPN RDYFVKFNCP EFTSLCPKTG 

        70         80         90        100        110        120 
QPDFATIYIS YIPEQRMVES KSLKLYLFSF RNHGDFHEDC MNVIMNDLIK LMDPRYIEVW 

       130        140        150        160 
GKFTPRGGIS IDPYCNYGRP GTKYEQMADY RMMNHDLYPE TIDNR 

« Hide

References

[1]"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. expand/collapse author list , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
Nature 423:81-86(2003) [PubMed: 12721629] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Ames / isolate Porton.
[2]"Bacillus anthracis comparative genomics."
Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Ames ancestor.
[3]"Complete genome sequence of Bacillus anthracis Sterne."
Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sterne.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016879 Genomic DNA. Translation: AAP25306.1.
AE017334 Genomic DNA. Translation: AAT30456.1.
AE017225 Genomic DNA. Translation: AAT53580.1.
RefSeqNP_843820.1.
YP_017981.1.
YP_027529.1.

3D structure databases

SMRQ81TC4. Positions 60-136.
ModBaseSearch...

Genome annotation databases

GeneID1086017.
2820070.
2850907.
GenomeReviewsGene locus BA_1362 in contig AE016879_GR.
Gene locus BAS1260 in contig AE017225_GR.
Gene locus GBAA_1362 in contig AE017334_GR.
KEGGban:BA1362.
bar:GBAA1362.
bat:BAS1260.
TIGRBA_1362.
GBAA_1362.

Phylogenomic databases

HOGENOMHBG294103.
OMAHESCINT.

Enzyme and pathway databases

BioCycBANT260799:BAS1260-MONOMER.
BANT261594:GBAA1362-MONOMER.
BRENDA1.7.1.13. 267517.

Family and domain databases

HAMAPMF_00818. QueF_type1.
[Tree]
InterProIPR016856. CN_OxRdtase_NADPH-dep_QueF.
IPR020602. GTP_CycHdrlase_I/CN_OxRdtase.
[Graphical view]
PfamPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
PIRSFPIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMsTIGR03139. QueF-II. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQUEF_BACAN
AccessionPrimary (citable) accession number: Q81TC4
Secondary accession number(s): Q6I1K1, Q6KVF0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: June 1, 2003
Last modified: February 9, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents