Reviewed, UniProtKB/Swiss-Prot Q81T69 (ILVC1_BACAN)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase 1
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase 1
    Alpha-keto-beta-hydroxylacil reductoisomerase 1

Gene names

Name:	ilvC1
Synonyms:	ilvC-1
Ordered Locus Names:	BA_1419, GBAA_1419, BAS1310

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Ketol-acid reductoisomerase 1 HAMAP MF_00435		PRO_0000151270
Sites
	Active site	107	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q81T69-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 9AC603888E73FF3E	FASTA	336	36,693
	10 20 30 40 50 60 MAKVYYEKDV TVNVLKEKKV AIIGYGSQGH AHAQNLRDNG FDVVVGLRKG KSWDKAKEDG 70 80 90 100 110 120 FSVYTVAEAA KQADVVMILL PDELQPEVYE EEIAPNLQAG NSLVFAHGFN VHFDQVKPPV 130 140 150 160 170 180 NVDVFLVAPK GPGHLVRRTF SEGGAVPALF AVYQDATGVA TEKALSYADG IGATRAGVLE 190 200 210 220 230 240 TTFKEETETD LFGEQAVLCG GVTALVKAGF ETLVDAGYQP ELAYFECLHE LKLIVDLMYE 250 260 270 280 290 300 GGLENMRYSV SDTAQWGDFV SGPRVVTEDT KKAMGTVLAE IQDGTFARGW IAEHKAGKPN 310 320 330 FYATNEKENE HEIEVVGRKL RGMMPFVQPR VKAGVK

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP25362.1. AE017334 Genomic DNA. Translation: AAT30515.1. AE017225 Genomic DNA. Translation: AAT53630.1.
RefSeq	NP_843876.1. YP_018040.1. YP_027579.1.
3D structure databases
SMR	Q81T69. Positions 3-327.
ModBase	Search...
Genome annotation databases
GeneID	1084747. 2816383. 2851032.
GenomeReviews	Gene locus BA_1419 in contig AE016879_GR. Gene locus BAS1310 in contig AE017225_GR. Gene locus GBAA_1419 in contig AE017334_GR.
KEGG	ban:BA1419. bar:GBAA1419. bat:BAS1310.
TIGR	BA_1419. GBAA_1419.
Phylogenomic databases
HOGENOM	HBG286525.
OMA	DSISNTA.
Enzyme and pathway databases
BioCyc	BANT260799:BAS1310-MONOMER. BANT261594:GBAA1419-MONOMER.
BRENDA	1.1.1.86. 267517.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC1_BACAN

Accession

Primary (citable) accession number: Q81T69
Secondary accession number(s): Q6I1F2, Q6KV98

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)