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Protein
Submitted name:

Microcin C7 self-immunity protein mccF

Gene

mccF

Organism
Bacillus anthracis
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei112 – 1121NucleophileUniRule annotation
Binding sitei112 – 1121AMPCombined sources
Active sitei235 – 2351Charge relay systemUniRule annotation
Binding sitei269 – 2691AMPCombined sources
Active sitei303 – 3031Charge relay systemUniRule annotation
Binding sitei303 – 3031AMPCombined sources

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi85 – 862AMPCombined sources
Nucleotide bindingi177 – 1804AMPCombined sources

GO - Molecular functioni

  • carboxypeptidase activity Source: UniProtKB-KW
  • hydrolase activity Source: TIGR
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  • bacteriocin immunity Source: TIGR
Complete GO annotation...

Keywords - Ligandi

Nucleotide-bindingCombined sources

Enzyme and pathway databases

BioCyciBANT260799:GJAJ-1878-MONOMER.
BANT261594:GJ7F-1953-MONOMER.

Protein family/group databases

MEROPSiS66.003.

Names & Taxonomyi

Protein namesi
Submitted name:
Microcin C7 self-immunity protein mccFImported
Submitted name:
Microcin immunity protein MccFImported
Submitted name:
Peptidase S66Imported
Gene namesi
Name:mccFImported
Ordered Locus Names:GBAA_1949Imported
ORF Names:ADK18_09510Imported, BASH2_03908Imported
OrganismiBacillus anthracisImported
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000054520 Componenti: Unassembled WGS sequence
  • UP000000594 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi301 ↔ 302Combined sources

Interactioni

Protein-protein interaction databases

STRINGi198094.BA_1949.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GJZX-ray2.10A/B1-333[»]
3SR3X-ray1.50A/B1-333[»]
3T5MX-ray1.75A/B1-333[»]
3TYXX-ray2.04A/B1-333[»]
3U1BX-ray1.60A/B1-333[»]
4JVOX-ray1.30A/B1-333[»]
4MI1X-ray1.40A/B1-333[»]
4MJXX-ray1.40A/B1-333[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4105EBE. Bacteria.
COG1619. LUCA.
HOGENOMiHOG000025480.
OMAiHTHPMLT.
OrthoDBiEOG664CJC.

Family and domain databases

Gene3Di3.40.50.10740. 1 hit.
3.50.30.60. 1 hit.
InterProiIPR027461. Carboxypeptidase_A_C.
IPR029062. Class_I_gatase-like.
IPR027478. LdcA_N_dom.
IPR003507. S66_fam.
[Graphical view]
PfamiPF02016. Peptidase_S66. 1 hit.
[Graphical view]
PIRSFiPIRSF028757. LD-carboxypeptidase. 1 hit.
SUPFAMiSSF141986. SSF141986. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q81RT8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLPKSLKYG DTIGIYSPSS PVTYTSPKRF ERAKSYLLQK GFHILEGSLT
60 70 80 90 100
GRYDYYRSGS IQERAKELNA LIRNPNVSCI MSTIGGMNSN SLLPYIDYDA
110 120 130 140 150
FQNNPKIMIG YSDATALLLG IYAKTGIPTF YGPALVPSFG EFEPFVDDTY
160 170 180 190 200
KYFLETLLHD QALPYNIKQP LFWSDEFINW EEKTKEKELR PNNWISVTNG
210 220 230 240 250
QATGRVIGGN LNTIQGIWGS PYMPCIQEGD ILFIEDSSKD AATIERSFSF
260 270 280 290 300
LKINGVFDKV SGIILGKHEQ FDDCGTNRKP YEILLEVLQN QRIPLLADFD
310 320 330
CCHTHPMITM PIGVQVKMDA TNKTIHILEK WKI
Length:333
Mass (Da):37,712
Last modified:June 1, 2003 - v1
Checksum:i4F198ABC1BAFC4CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017334 Genomic DNA. Translation: AAT31068.1.
AP014833 Genomic DNA. Translation: BAR77315.1.
LGCD01000021 Genomic DNA. Translation: KOS29229.1.
RefSeqiWP_001130814.1. NZ_LHUO01000023.1.

Genome annotation databases

EnsemblBacteriaiAAT31068; AAT31068; GBAA_1949.
AJH87138; AJH87138; BF27_5605.
KOM62600; KOM62600; AB168_09475.
KOM65973; KOM65973; AB166_15330.
KOM73943; KOM73943; AB165_02915.
KOM79496; KOM79496; AB167_02840.
KOM85314; KOM85314; AB164_01525.
KOM90401; KOM90401; AB169_26665.
KON02478; KON02478; AB170_25605.
KON19396; KON19396; AB171_03825.
KON23716; KON23716; AB163_13970.
KOR56118; KOR56118; ADT21_23865.
KOR64375; KOR64375; ADT20_23255.
KOS29229; KOS29229; ADK18_09510.
KOS29362; KOS29362; ADK17_07400.
KEGGibanh:HYU01_09770.
bar:GBAA_1949.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017334 Genomic DNA. Translation: AAT31068.1.
AP014833 Genomic DNA. Translation: BAR77315.1.
LGCD01000021 Genomic DNA. Translation: KOS29229.1.
RefSeqiWP_001130814.1. NZ_LHUO01000023.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GJZX-ray2.10A/B1-333[»]
3SR3X-ray1.50A/B1-333[»]
3T5MX-ray1.75A/B1-333[»]
3TYXX-ray2.04A/B1-333[»]
3U1BX-ray1.60A/B1-333[»]
4JVOX-ray1.30A/B1-333[»]
4MI1X-ray1.40A/B1-333[»]
4MJXX-ray1.40A/B1-333[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198094.BA_1949.

Protein family/group databases

MEROPSiS66.003.

Protocols and materials databases

DNASUi1086086.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT31068; AAT31068; GBAA_1949.
AJH87138; AJH87138; BF27_5605.
KOM62600; KOM62600; AB168_09475.
KOM65973; KOM65973; AB166_15330.
KOM73943; KOM73943; AB165_02915.
KOM79496; KOM79496; AB167_02840.
KOM85314; KOM85314; AB164_01525.
KOM90401; KOM90401; AB169_26665.
KON02478; KON02478; AB170_25605.
KON19396; KON19396; AB171_03825.
KON23716; KON23716; AB163_13970.
KOR56118; KOR56118; ADT21_23865.
KOR64375; KOR64375; ADT20_23255.
KOS29229; KOS29229; ADK18_09510.
KOS29362; KOS29362; ADK17_07400.
KEGGibanh:HYU01_09770.
bar:GBAA_1949.

Phylogenomic databases

eggNOGiENOG4105EBE. Bacteria.
COG1619. LUCA.
HOGENOMiHOG000025480.
OMAiHTHPMLT.
OrthoDBiEOG664CJC.

Enzyme and pathway databases

BioCyciBANT260799:GJAJ-1878-MONOMER.
BANT261594:GJ7F-1953-MONOMER.

Family and domain databases

Gene3Di3.40.50.10740. 1 hit.
3.50.30.60. 1 hit.
InterProiIPR027461. Carboxypeptidase_A_C.
IPR029062. Class_I_gatase-like.
IPR027478. LdcA_N_dom.
IPR003507. S66_fam.
[Graphical view]
PfamiPF02016. Peptidase_S66. 1 hit.
[Graphical view]
PIRSFiPIRSF028757. LD-carboxypeptidase. 1 hit.
SUPFAMiSSF141986. SSF141986. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames ancestorImported and Ames AncestorImported.
  2. "Structural and functional characterization of microcin C resistance peptidase MccF from Bacillus anthracis."
    Nocek B., Tikhonov A., Babnigg G., Gu M., Zhou M., Makarova K.S., Vondenhoff G., Van Aerschot A., Kwon K., Anderson W.F., Severinov K., Joachimiak A.
    J. Mol. Biol. 420:366-383(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) IN COMPLEX WITH AMP, DISULFIDE BONDS.
  3. "Crystal structure of the double mutant (S112A, H303A) of B.anthracis mycrocine immunity protein (MccF) with alanyl sulfamoyl adenylates."
    Nocek B., Tikhonov A., Severinov K., Anderson W.F., Joachimiak A.
    Submitted (MAR-2013) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS).
  4. "Crystal structure of the double mutant (S112A, H303A) of B.anthracis mycrocine immunity protein (MccF) with aspartyl sulfamoyl adenylates."
    Nocek B., Severinov K., Anderson W.F., Joachimiak A.
    Submitted (AUG-2013) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS).
  5. "Crystal structure of the double mutant (S112A, H303A) of B.anthracis mycrocine immunity protein (MccF)."
    Nocek B., Anderson W.F., Severinov K., Joachimiak A.
    Submitted (SEP-2013) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS).
  6. "Bacillus anthracis whole genome sequence."
    Okutani A., Morikawa S., Inoue S.
    Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Shikan-NIIDImported.
  7. "Draft genome sequences of two South African Bacillus anthracis strains."
    Lekota K.E., Mafofo J., Madoroba E., Rees J., van Heerden H., Muchadeyi F.C.
    Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 3631_1CImported.

Entry informationi

Entry nameiQ81RT8_BACAN
AccessioniPrimary (citable) accession number: Q81RT8
Secondary accession number(s): E9R7Y8
, E9R7Y9, Q6I015, Q6KTZ4
Entry historyi
Integrated into UniProtKB/TrEMBL: June 1, 2003
Last sequence update: June 1, 2003
Last modified: July 6, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.