Reviewed, UniProtKB/Swiss-Prot Q81RS0 (KHSE_BACAN)

Names and origin

Protein names

Recommended name:
    Homoserine kinase
      Short name=HSK
      Short name=HK
    EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	BA_1970, GBAA_1970, BAS1827

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro threonine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP homoserine kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Homoserine kinase HAMAP MF_00384		PRO_0000156547
Regions
	Nucleotide binding	82 – 92	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q81RS0-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: C848C1B8FDBEE0D0	FASTA	297	32,134
	10 20 30 40 50 60 MIPLSIRVPA STANVGPGFD SVGIALSLYL HVVVKEKSDK WQVIHSFEDS IPTDDKNLIV 70 80 90 100 110 120 STACKVCPSL SPHIIEVTSN IPLTRGLGSS ASAIVAGIEL ANQLGKLNLT IDQKVQIATN 130 140 150 160 170 180 FEGHPDNVAA SILGGTVIGA LDGKNVSVVR IESKELGVIS LIPNEELNTD ESRSVLPDVF 190 200 210 220 230 240 PFHEAVKASA ISNVLVAALC QKKWKVVGEM MERDHFHEPY RLELVPLLPS IRKCAKEFGA 250 260 270 280 290 YGTALSGAGP SIFILTPYEK RQEIAEQLAR VFTSMKVCEL EIDHRGITVN KKEHIGL

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016879 Genomic DNA. Translation: AAP25862.1. AE017334 Genomic DNA. Translation: AAT31089.1. AE017225 Genomic DNA. Translation: AAT54142.1.
RefSeq	NP_844376.1. YP_018614.1. YP_028091.1.
3D structure databases
HSSP	HSSP built from PDB template 1H72 based on UniProtKB Q58504.
ModBase	Search...
Genome annotation databases
GeneID	1085942. 2816989. 2849936.
GenomeReviews	Gene locus BA_1970 in contig AE016879_GR. Gene locus BAS1827 in contig AE017225_GR. Gene locus GBAA_1970 in contig AE017334_GR.
KEGG	ban:BA1970. bar:GBAA1970. bat:BAS1827.
TIGR	BA_1970. GBAA_1970.
Phylogenomic databases
HOGENOM	Q81RS0.
OMA	FDCLGAA.
Enzyme and pathway databases
BioCyc	BANT260799:BAS1827-MON. BANT261594:GBAA1970-MON.
BRENDA	2.7.1.39. 267517.
Family and domain databases
HAMAP	MF_00384. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP_bd_CS. IPR000870. Homoserine_kin. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
TIGRFAMs	TIGR00191. thrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_BACAN

Accession

Primary (citable) accession number: Q81RS0
Secondary accession number(s): Q6HZZ7, Q6KTX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)