NH(3)-dependent NAD(+) synthetase - Bacillus anthracis

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Reviewed, UniProtKB/Swiss-Prot Q81RP3 (NADE_BACAN)

Last modified November 3, 2009. Version 46. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	BA_1998, GBAA_1998, BAS1855

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD⁺ + NH₃ = AMP + diphosphate + NAD⁺. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

272

NH(3)-dependent NAD(+) synthetase HAMAP MF_00193

PRO_0000152156

Regions

Nucleotide binding

8

ATP By similarity

Sites

Active site

1

By similarity

Secondary structure

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272

Helix Strand Turn

Sequences

Sequence

Length

Mass (Da)

Tools

Q81RP3-1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: B58A9792E8075B98
Show »

272

30,101

        10         20         30         40         50         60 
MTLQEQIMKA LHVQPVIDPK AEIRKRVDFL KDYVKKTGAK GFVLGISGGQ DSTLAGRLAQ 

        70         80         90        100        110        120 
LAVEEIRNEG GNATFIAVRL PYKVQKDEDD AQLALQFIQA DQSVAFDIAS TVDAFSNQYE 

       130        140        150        160        170        180 
NLLDESLTDF NKGNVKARIR MVTQYAIGGQ KGLLVIGTDH AAEAVTGFFT KFGDGGADLL 

       190        200        210        220        230        240 
PLTGLTKRQG RALLQELGAD ERLYLKMPTA DLLDEKPGQA DETELGITYD QLDDYLEGKT 

       250        260        270 
VPADVAEKIE KRYTVSEHKR QVPASMFDDW WK

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.
+	Additional computationally mapped references.

Cross-references

Sequence databases

AE016879 Genomic DNA. Translation: AAP25889.1.
AE017334 Genomic DNA. Translation: AAT31119.1.
AE017225 Genomic DNA. Translation: AAT54170.1.

RefSeq

NP_844403.1.
YP_018644.1.
YP_028119.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PZ8	X-ray	2.00	A/B	1-272	[»]
2PZA	X-ray	2.40	A/B	1-272	[»]
2PZB	X-ray	1.90	A/B/C/D	1-272	[»]

ModBase

Genome annotation databases

GeneID

1087061.
2817738.
2851218.

GenomeReviews

Gene locus BA_1998 in contig AE016879_GR.
Gene locus BAS1855 in contig AE017225_GR.
Gene locus GBAA_1998 in contig AE017334_GR.

KEGG

ban:BA1998.
bar:GBAA1998.
bat:BAS1855.

TIGR

BA_1998.
GBAA_1998.

Phylogenomic databases

HOGENOM

OMA

Enzyme and pathway databases

BioCyc

BANT260799:BAS1855-MON.
BANT261594:GBAA1998-MON.

BRENDA

6.3.1.5. 267517.

Family and domain databases

HAMAP

MF_00193.
[Tree]

InterPro

IPR003694. NAD_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]

Gene3D

G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.

Pfam

PF02540. NAD_synthase. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00552. nadE. 1 hit.

ProtoNet

Entry information

Entry name

NADE_BACAN

Accession

Primary (citable) accession number: Q81RP3
Secondary accession number(s): Q6HZW9, Q6KTU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents