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Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NH(3)-dependent NAD(+) synthetase (nadE), NH(3)-dependent NAD(+) synthetase (nadE), NH(3)-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei47 – 471UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi45 – 528ATPUniRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • NAD+ synthase (glutamine-hydrolyzing) activity Source: TIGR
  • NAD+ synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  • asexual sporulation Source: TIGR
  • NAD biosynthetic process Source: UniProtKB-HAMAP
  • NADH metabolic process Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciANTHRA:NADE-MONOMER.
BANT260799:GJAJ-1924-MONOMER.
BANT261594:GJ7F-1999-MONOMER.
BRENDAi6.3.1.5. 634.
UniPathwayiUPA00253; UER00333.

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.1.5UniRule annotation)
Gene namesi
Name:nadEUniRule annotation
Ordered Locus Names:BA_1998, GBAA_1998, BAS1855
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL5271.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 272272NH(3)-dependent NAD(+) synthetasePRO_0000152156Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi198094.BA_1998.

Chemistry

BindingDBiQ81RP3.

Structurei

Secondary structure

1
272
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 119Combined sources
Helixi19 – 3719Combined sources
Beta strandi41 – 455Combined sources
Helixi50 – 6819Combined sources
Beta strandi74 – 796Combined sources
Beta strandi82 – 843Combined sources
Helixi89 – 9810Combined sources
Beta strandi101 – 1055Combined sources
Helixi109 – 12214Combined sources
Helixi129 – 15123Combined sources
Beta strandi154 – 1563Combined sources
Helixi162 – 1665Combined sources
Turni171 – 1755Combined sources
Beta strandi178 – 1803Combined sources
Turni181 – 1844Combined sources
Helixi187 – 19610Combined sources
Helixi201 – 2033Combined sources
Beta strandi214 – 2163Combined sources
Helixi221 – 2255Combined sources
Helixi229 – 2368Combined sources
Helixi243 – 25513Combined sources
Helixi256 – 2605Combined sources
Helixi267 – 2726Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PZ8X-ray2.00A/B1-272[»]
2PZAX-ray2.40A/B1-272[»]
2PZBX-ray1.90A/B/C/D1-272[»]
ProteinModelPortaliQ81RP3.
SMRiQ81RP3. Positions 1-272.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ81RP3.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4K. Bacteria.
COG0171. LUCA.
HOGENOMiHOG000238070.
KOiK01916.
OMAiLVSMPES.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE. 1 hit.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q81RP3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLQEQIMKA LHVQPVIDPK AEIRKRVDFL KDYVKKTGAK GFVLGISGGQ
60 70 80 90 100
DSTLAGRLAQ LAVEEIRNEG GNATFIAVRL PYKVQKDEDD AQLALQFIQA
110 120 130 140 150
DQSVAFDIAS TVDAFSNQYE NLLDESLTDF NKGNVKARIR MVTQYAIGGQ
160 170 180 190 200
KGLLVIGTDH AAEAVTGFFT KFGDGGADLL PLTGLTKRQG RALLQELGAD
210 220 230 240 250
ERLYLKMPTA DLLDEKPGQA DETELGITYD QLDDYLEGKT VPADVAEKIE
260 270
KRYTVSEHKR QVPASMFDDW WK
Length:272
Mass (Da):30,101
Last modified:June 1, 2003 - v1
Checksum:iB58A9792E8075B98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP25889.1.
AE017334 Genomic DNA. Translation: AAT31119.1.
AE017225 Genomic DNA. Translation: AAT54170.1.
RefSeqiNP_844403.1. NC_003997.3.
WP_000174879.1. NZ_LHUO01000023.1.
YP_028119.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP25889; AAP25889; BA_1998.
AAT31119; AAT31119; GBAA_1998.
AAT54170; AAT54170; BAS1855.
GeneIDi1087061.
2851218.
KEGGiban:BA_1998.
bar:GBAA_1998.
bat:BAS1855.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP25889.1.
AE017334 Genomic DNA. Translation: AAT31119.1.
AE017225 Genomic DNA. Translation: AAT54170.1.
RefSeqiNP_844403.1. NC_003997.3.
WP_000174879.1. NZ_LHUO01000023.1.
YP_028119.1. NC_005945.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PZ8X-ray2.00A/B1-272[»]
2PZAX-ray2.40A/B1-272[»]
2PZBX-ray1.90A/B/C/D1-272[»]
ProteinModelPortaliQ81RP3.
SMRiQ81RP3. Positions 1-272.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198094.BA_1998.

Chemistry

BindingDBiQ81RP3.
ChEMBLiCHEMBL5271.

Protocols and materials databases

DNASUi1087061.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP25889; AAP25889; BA_1998.
AAT31119; AAT31119; GBAA_1998.
AAT54170; AAT54170; BAS1855.
GeneIDi1087061.
2851218.
KEGGiban:BA_1998.
bar:GBAA_1998.
bat:BAS1855.

Phylogenomic databases

eggNOGiENOG4105C4K. Bacteria.
COG0171. LUCA.
HOGENOMiHOG000238070.
KOiK01916.
OMAiLVSMPES.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.
BioCyciANTHRA:NADE-MONOMER.
BANT260799:GJAJ-1924-MONOMER.
BANT261594:GJ7F-1999-MONOMER.
BRENDAi6.3.1.5. 634.

Miscellaneous databases

EvolutionaryTraceiQ81RP3.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE. 1 hit.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADE_BACAN
AccessioniPrimary (citable) accession number: Q81RP3
Secondary accession number(s): Q6HZW9, Q6KTU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 2003
Last modified: September 7, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.