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Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NH(3)-dependent NAD(+) synthetase (nadE), NH(3)-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei47UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi45 – 52ATPUniRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • NAD+ synthase (glutamine-hydrolyzing) activity Source: TIGR
  • NAD+ synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  • asexual sporulation Source: TIGR
  • NAD biosynthetic process Source: UniProtKB-HAMAP
  • NADH metabolic process Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciANTHRA:NADE-MONOMER.
BRENDAi6.3.1.5. 634.
UniPathwayiUPA00253; UER00333.

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.1.5UniRule annotation)
Gene namesi
Name:nadEUniRule annotation
Ordered Locus Names:BA_1998, GBAA_1998, BAS1855
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5271.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001521561 – 272NH(3)-dependent NAD(+) synthetaseAdd BLAST272

Interactioni

Protein-protein interaction databases

STRINGi198094.BA_1998.

Chemistry databases

BindingDBiQ81RP3.

Structurei

Secondary structure

1272
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 11Combined sources9
Helixi19 – 37Combined sources19
Beta strandi41 – 45Combined sources5
Helixi50 – 68Combined sources19
Beta strandi74 – 79Combined sources6
Beta strandi82 – 84Combined sources3
Helixi89 – 98Combined sources10
Beta strandi101 – 105Combined sources5
Helixi109 – 122Combined sources14
Helixi129 – 151Combined sources23
Beta strandi154 – 156Combined sources3
Helixi162 – 166Combined sources5
Turni171 – 175Combined sources5
Beta strandi178 – 180Combined sources3
Turni181 – 184Combined sources4
Helixi187 – 196Combined sources10
Helixi201 – 203Combined sources3
Beta strandi214 – 216Combined sources3
Helixi221 – 225Combined sources5
Helixi229 – 236Combined sources8
Helixi243 – 255Combined sources13
Helixi256 – 260Combined sources5
Helixi267 – 272Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PZ8X-ray2.00A/B1-272[»]
2PZAX-ray2.40A/B1-272[»]
2PZBX-ray1.90A/B/C/D1-272[»]
ProteinModelPortaliQ81RP3.
SMRiQ81RP3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ81RP3.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4K. Bacteria.
COG0171. LUCA.
HOGENOMiHOG000238070.
KOiK01916.
OMAiLVSMPES.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE. 1 hit.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q81RP3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLQEQIMKA LHVQPVIDPK AEIRKRVDFL KDYVKKTGAK GFVLGISGGQ
60 70 80 90 100
DSTLAGRLAQ LAVEEIRNEG GNATFIAVRL PYKVQKDEDD AQLALQFIQA
110 120 130 140 150
DQSVAFDIAS TVDAFSNQYE NLLDESLTDF NKGNVKARIR MVTQYAIGGQ
160 170 180 190 200
KGLLVIGTDH AAEAVTGFFT KFGDGGADLL PLTGLTKRQG RALLQELGAD
210 220 230 240 250
ERLYLKMPTA DLLDEKPGQA DETELGITYD QLDDYLEGKT VPADVAEKIE
260 270
KRYTVSEHKR QVPASMFDDW WK
Length:272
Mass (Da):30,101
Last modified:June 1, 2003 - v1
Checksum:iB58A9792E8075B98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP25889.1.
AE017334 Genomic DNA. Translation: AAT31119.1.
AE017225 Genomic DNA. Translation: AAT54170.1.
RefSeqiNP_844403.1. NC_003997.3.
WP_000174879.1. NZ_LHUO01000023.1.
YP_028119.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP25889; AAP25889; BA_1998.
AAT31119; AAT31119; GBAA_1998.
AAT54170; AAT54170; BAS1855.
GeneIDi1087061.
2851218.
KEGGiban:BA_1998.
bar:GBAA_1998.
bat:BAS1855.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP25889.1.
AE017334 Genomic DNA. Translation: AAT31119.1.
AE017225 Genomic DNA. Translation: AAT54170.1.
RefSeqiNP_844403.1. NC_003997.3.
WP_000174879.1. NZ_LHUO01000023.1.
YP_028119.1. NC_005945.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PZ8X-ray2.00A/B1-272[»]
2PZAX-ray2.40A/B1-272[»]
2PZBX-ray1.90A/B/C/D1-272[»]
ProteinModelPortaliQ81RP3.
SMRiQ81RP3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198094.BA_1998.

Chemistry databases

BindingDBiQ81RP3.
ChEMBLiCHEMBL5271.

Protocols and materials databases

DNASUi1087061.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP25889; AAP25889; BA_1998.
AAT31119; AAT31119; GBAA_1998.
AAT54170; AAT54170; BAS1855.
GeneIDi1087061.
2851218.
KEGGiban:BA_1998.
bar:GBAA_1998.
bat:BAS1855.

Phylogenomic databases

eggNOGiENOG4105C4K. Bacteria.
COG0171. LUCA.
HOGENOMiHOG000238070.
KOiK01916.
OMAiLVSMPES.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.
BioCyciANTHRA:NADE-MONOMER.
BRENDAi6.3.1.5. 634.

Miscellaneous databases

EvolutionaryTraceiQ81RP3.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE. 1 hit.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADE_BACAN
AccessioniPrimary (citable) accession number: Q81RP3
Secondary accession number(s): Q6HZW9, Q6KTU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.