Reviewed, UniProtKB/Swiss-Prot Q81RK6 (NAMA_BACAN)

Names and origin

Protein names

Recommended name:
    NADPH dehydrogenase
    EC=1.6.99.1
Alternative name(s):
    Xenobiotic reductase

Gene names

Name:	namA
Ordered Locus Names:	BA_2038, GBAA2038, BAS1891

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes By similarity.
Catalytic activity	NADPH + acceptor = NADP(+) + reduced acceptor.
Cofactor	FMN By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.

Ontologies

Keywords
Ligand	FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	NADPH dehydrogenase		PRO_0000216112
Regions
	Nucleotide binding	23 – 27	5	FMN By similarity
Sites
	Binding site	28	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	215	1	FMN By similarity
	Binding site	308	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81RK6-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: C6DF787B1CEAD97E	FASTA	345	38,459
	10 20 30 40 50 60 MNSELFSPYT IKDVTLKNRI VMSPMCMYSS ENEDGQVTNF HLIHYGTRAA GQVGLVMIEA 70 80 90 100 110 120 TAVLPEGRIS NKDLGIWDDS LIEGLHKTTT FIHDNGAKAA IQLAHAGRKA ELETDALAPS 130 140 150 160 170 180 AVPFNETMKI PVEMSIHQIK NTILAFQQAA IRSKQAGFDV IEIHGAHGYL INEFLSPLSN 190 200 210 220 230 240 KRTDEYGGSP ENRYRFLREI IDSINEVWNG PLFVRISAND YHPDGLTVQD YVQYTKWMKE 250 260 270 280 290 300 QGVDLIDCSS GAVVPARIDV YPGYQVQYAK HIKEHANIAT GAVGLITTGA QAEQILNNNE 310 320 330 340 ADLIFIGREL LRNPYFPRIA ANELGFELEE PHQYERAPGK ISTNK

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016879 Genomic DNA. Translation: AAP25926.1. AE017334 Genomic DNA. Translation: AAT31152.1. AE017225 Genomic DNA. Translation: AAT54206.1.
RefSeq	NP_844440.1. YP_018677.1. YP_028155.1.
3D structure databases
HSSP	HSSP built from PDB template 1GWJ based on UniProtKB Q51990.
ModBase	Search...
Genome annotation databases
GeneID	1085848. 2814876. 2852207.
GenomeReviews	Gene locus BA_2038 in contig AE016879_GR. Gene locus BAS1891 in contig AE017225_GR. Gene locus GBAA2038 in contig AE017334_GR.
KEGG	ban:BA2038. bar:GBAA2038. bat:BAS1891.
TIGR	BA_2038. GBAA2038.
Phylogenomic databases
HOGENOM	Q81RK6.
Enzyme and pathway databases
BioCyc	BANT260799:BAS1891-MON. BANT261594:GBAA2038-MON.
Family and domain databases
HAMAP	MF_01614. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR001155. OxRdtase_FMN_N. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00724. Oxidored_FMN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAMA_BACAN

Accession

Primary (citable) accession number: Q81RK6
Secondary accession number(s): Q6HZT3, Q6KTR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)