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Q81R75

- SYI2_BACAN

UniProt

Q81R75 - SYI2_BACAN

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Protein

Isoleucine--tRNA ligase 2

Gene

ileS2

Organism
Bacillus anthracis
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei593 – 5931ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBANT260799:GJAJ-2095-MONOMER.
BANT261594:GJ7F-2173-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase 2UniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetase 2UniRule annotation
Short name:
IleRS 2UniRule annotation
Gene namesi
Name:ileS2UniRule annotation
Ordered Locus Names:BA_2181, GBAA_2181, BAS2027
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000000427: Chromosome, UP000000594: Chromosome, UP000005639: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10331033Isoleucine--tRNA ligase 2PRO_0000098515Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

IntActiQ81R75. 1 interaction.
STRINGi198094.BA_2181.

Structurei

3D structure databases

ProteinModelPortaliQ81R75.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionAdd
BLAST
Motifi590 – 5945"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiSEGMEPN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81R75-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKVDVKESA VGREMRIRKQ WNEQNIFEQS IQNREGAQSF VFYEGPPTAN
60 70 80 90 100
GLPHVGHALG RTIKDVVARY KTMAGYKVLR KAGWDTHGLP VELGVEKQLG
110 120 130 140 150
ISGKHEIEEY GIEPFIKKCK ESVFTYEKQW REFTESIGYW VDMDDPYVTL
160 170 180 190 200
ENPYIESVWH ILGTIHEKGL LYKGHRVSPY CPSCQTSLSS HEVAQGYKTV
210 220 230 240 250
KDLSATVKFK VKDSENEYFL GWTTTPWTLP ANVALAVHPN MEYVKAKQEG
260 270 280 290 300
HVYIVAKERV QDVLKENYEV LSVHKGEELL NISYTAPFPM KEVTNGYRVI
310 320 330 340 350
GADFVTADSG TGLVHIAPAY GEDDYRVVQS EGLSFLHVVD EKGEYTEAVP
360 370 380 390 400
FLKGKFVKDC DVDIVRYLAK EDLLYHKEKY EHSYPHCWRC DSPLLYYAGE
410 420 430 440 450
SWLIRTTAIK DTFLQNNDTV TWYPDHMKHG RFGKFLENMV DWNISRNRYW
460 470 480 490 500
GTPLNVWECE RCDHQFAPKS IADLRKHSMK ETPEDLELHK PYVDEVQVCC
510 520 530 540 550
EKCGSTMNRT PEVIDVWFDS GSMPFAQYHY PFENKELFEE QFPADVIAEG
560 570 580 590 600
IDQTRGWFYS LLAVSALYTG KVPYKRVLSL GHVLDEEGQK MSKSKGNALD
610 620 630 640 650
PVDLVGKFGA DALRWALLVD SALWNAKRFS ERTVLEAKSK FVDTLVNVYS
660 670 680 690 700
FYVLYANLDE YNPNETYDVK RTKLDEWVLS RLHSTTKKVR TALDDYQFTN
710 720 730 740 750
AAREIAALVD EVSNWYVRRS RNRFWESGMN AEKAAAYETL HDVLVTISKL
760 770 780 790 800
IAPFTPFVAE DVHLNLTGSS VHLEDYPVVN ESLLQPKLEA EMDAVLQVVE
810 820 830 840 850
LGRSNRNQHS LKVKQPLAEL VLLEHNENDM DWESYRDIVM DELNVKAFHV
860 870 880 890 900
ELDETKYTSY QLKLNFKKAG PKFGKNVNAV NGWLKQLSQD EVQNFVSTER
910 920 930 940 950
AVYEVAPGEE IVVTTEDVLV EKVAKSGFSN TTNGQYTVML DTNVTEELLQ
960 970 980 990 1000
EGVAREFIRA VQEYRKQLNL PVNLRVDVIL DTEEELQQTL TNHKELLEEN
1010 1020 1030
LLVKQFTFGH LTNEDDELSL GETKVRIKLS TAQ
Length:1,033
Mass (Da):118,653
Last modified:June 1, 2003 - v1
Checksum:i4E393F801CC74A8B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016879 Genomic DNA. Translation: AAP26060.1.
AE017334 Genomic DNA. Translation: AAT31298.1.
AE017225 Genomic DNA. Translation: AAT54341.1.
RefSeqiNP_844574.1. NC_003997.3.
WP_000754882.1. NZ_JHDR01000084.1.
YP_018823.1. NC_007530.2.
YP_028290.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP26060; AAP26060; BA_2181.
AAT31298; AAT31298; GBAA_2181.
AAT54341; AAT54341; BAS2027.
GeneIDi1085720.
2815690.
2849029.
KEGGiban:BA_2181.
bar:GBAA_2181.
bat:BAS2027.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016879 Genomic DNA. Translation: AAP26060.1 .
AE017334 Genomic DNA. Translation: AAT31298.1 .
AE017225 Genomic DNA. Translation: AAT54341.1 .
RefSeqi NP_844574.1. NC_003997.3.
WP_000754882.1. NZ_JHDR01000084.1.
YP_018823.1. NC_007530.2.
YP_028290.1. NC_005945.1.

3D structure databases

ProteinModelPortali Q81R75.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q81R75. 1 interaction.
STRINGi 198094.BA_2181.

Protocols and materials databases

DNASUi 1085720.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP26060 ; AAP26060 ; BA_2181 .
AAT31298 ; AAT31298 ; GBAA_2181 .
AAT54341 ; AAT54341 ; BAS2027 .
GeneIDi 1085720.
2815690.
2849029.
KEGGi ban:BA_2181.
bar:GBAA_2181.
bat:BAS2027.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi SEGMEPN.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BANT260799:GJAJ-2095-MONOMER.
BANT261594:GJ7F-2173-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
    Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
    , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
    Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames / isolate Porton.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames ancestor.
  3. "Complete genome sequence of Bacillus anthracis Sterne."
    Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sterne.

Entry informationi

Entry nameiSYI2_BACAN
AccessioniPrimary (citable) accession number: Q81R75
Secondary accession number(s): Q6HZE8, Q6KTD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3