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Q81R75

- SYI2_BACAN

UniProt

Q81R75 - SYI2_BACAN

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Protein
Isoleucine--tRNA ligase 2
Gene
ileS2, BA_2181, GBAA_2181, BAS2027
Organism
Bacillus anthracis
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei593 – 5931ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBANT260799:GJAJ-2095-MONOMER.
BANT261594:GJ7F-2173-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase 2 (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase 2
Short name:
IleRS 2
Gene namesi
Name:ileS2
Ordered Locus Names:BA_2181, GBAA_2181, BAS2027
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000000427: Chromosome, UP000000594: Chromosome, UP000005639: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10331033Isoleucine--tRNA ligase 2UniRule annotation
PRO_0000098515Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

IntActiQ81R75. 1 interaction.
STRINGi198094.BA_2181.

Structurei

3D structure databases

ProteinModelPortaliQ81R75.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionUniRule annotation
Add
BLAST
Motifi590 – 5945"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiSEGMEPN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81R75-1 [UniParc]FASTAAdd to Basket

« Hide

MKKVDVKESA VGREMRIRKQ WNEQNIFEQS IQNREGAQSF VFYEGPPTAN     50
GLPHVGHALG RTIKDVVARY KTMAGYKVLR KAGWDTHGLP VELGVEKQLG 100
ISGKHEIEEY GIEPFIKKCK ESVFTYEKQW REFTESIGYW VDMDDPYVTL 150
ENPYIESVWH ILGTIHEKGL LYKGHRVSPY CPSCQTSLSS HEVAQGYKTV 200
KDLSATVKFK VKDSENEYFL GWTTTPWTLP ANVALAVHPN MEYVKAKQEG 250
HVYIVAKERV QDVLKENYEV LSVHKGEELL NISYTAPFPM KEVTNGYRVI 300
GADFVTADSG TGLVHIAPAY GEDDYRVVQS EGLSFLHVVD EKGEYTEAVP 350
FLKGKFVKDC DVDIVRYLAK EDLLYHKEKY EHSYPHCWRC DSPLLYYAGE 400
SWLIRTTAIK DTFLQNNDTV TWYPDHMKHG RFGKFLENMV DWNISRNRYW 450
GTPLNVWECE RCDHQFAPKS IADLRKHSMK ETPEDLELHK PYVDEVQVCC 500
EKCGSTMNRT PEVIDVWFDS GSMPFAQYHY PFENKELFEE QFPADVIAEG 550
IDQTRGWFYS LLAVSALYTG KVPYKRVLSL GHVLDEEGQK MSKSKGNALD 600
PVDLVGKFGA DALRWALLVD SALWNAKRFS ERTVLEAKSK FVDTLVNVYS 650
FYVLYANLDE YNPNETYDVK RTKLDEWVLS RLHSTTKKVR TALDDYQFTN 700
AAREIAALVD EVSNWYVRRS RNRFWESGMN AEKAAAYETL HDVLVTISKL 750
IAPFTPFVAE DVHLNLTGSS VHLEDYPVVN ESLLQPKLEA EMDAVLQVVE 800
LGRSNRNQHS LKVKQPLAEL VLLEHNENDM DWESYRDIVM DELNVKAFHV 850
ELDETKYTSY QLKLNFKKAG PKFGKNVNAV NGWLKQLSQD EVQNFVSTER 900
AVYEVAPGEE IVVTTEDVLV EKVAKSGFSN TTNGQYTVML DTNVTEELLQ 950
EGVAREFIRA VQEYRKQLNL PVNLRVDVIL DTEEELQQTL TNHKELLEEN 1000
LLVKQFTFGH LTNEDDELSL GETKVRIKLS TAQ 1033
Length:1,033
Mass (Da):118,653
Last modified:June 1, 2003 - v1
Checksum:i4E393F801CC74A8B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016879 Genomic DNA. Translation: AAP26060.1.
AE017334 Genomic DNA. Translation: AAT31298.1.
AE017225 Genomic DNA. Translation: AAT54341.1.
RefSeqiNP_844574.1. NC_003997.3.
YP_018823.1. NC_007530.2.
YP_028290.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP26060; AAP26060; BA_2181.
AAT31298; AAT31298; GBAA_2181.
AAT54341; AAT54341; BAS2027.
GeneIDi1085720.
2815690.
2849029.
KEGGiban:BA_2181.
bar:GBAA_2181.
bat:BAS2027.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016879 Genomic DNA. Translation: AAP26060.1 .
AE017334 Genomic DNA. Translation: AAT31298.1 .
AE017225 Genomic DNA. Translation: AAT54341.1 .
RefSeqi NP_844574.1. NC_003997.3.
YP_018823.1. NC_007530.2.
YP_028290.1. NC_005945.1.

3D structure databases

ProteinModelPortali Q81R75.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q81R75. 1 interaction.
STRINGi 198094.BA_2181.

Protocols and materials databases

DNASUi 1085720.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP26060 ; AAP26060 ; BA_2181 .
AAT31298 ; AAT31298 ; GBAA_2181 .
AAT54341 ; AAT54341 ; BAS2027 .
GeneIDi 1085720.
2815690.
2849029.
KEGGi ban:BA_2181.
bar:GBAA_2181.
bat:BAS2027.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi SEGMEPN.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BANT260799:GJAJ-2095-MONOMER.
BANT261594:GJ7F-2173-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
    Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
    , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
    Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames / isolate Porton.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames ancestor.
  3. "Complete genome sequence of Bacillus anthracis Sterne."
    Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sterne.

Entry informationi

Entry nameiSYI2_BACAN
AccessioniPrimary (citable) accession number: Q81R75
Secondary accession number(s): Q6HZE8, Q6KTD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi