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Protein

Thymidylate synthase

Gene

thyA

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei25dUMPUniRule annotation1
Active sitei200NucleophileUniRule annotation1
Binding sitei2235,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei231dUMPUniRule annotation1
Binding sitei3175,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi180 – 181dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi220 – 223dUMPUniRule annotation4
Nucleotide bindingi261 – 263dUMPUniRule annotation3

GO - Molecular functioni

  • thymidylate synthase activity Source: TIGR

GO - Biological processi

  • 2'-deoxyribonucleotide biosynthetic process Source: TIGR
  • dTMP biosynthetic process Source: UniProtKB-HAMAP
  • dTTP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

BioCyciANTHRA:THYA-MONOMER.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:BA_2236, GBAA_2236, BAS2082
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001409161 – 318Thymidylate synthaseAdd BLAST318

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi198094.BA_2236.

Structurei

3D structure databases

ProteinModelPortaliQ81R23.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V. Bacteria.
COG0207. LUCA.
HOGENOMiHOG000257899.
KOiK00560.
OMAiIVYELLW.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 2 hits.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81R23-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHAENEYLN LCRHVMEHGT KKEDRTGTGT VSVFGYQMRF DLSKGFPLLT
60 70 80 90 100
TKRVPFRLVA SELLWFMKGD TNIRYLLQHN NNIWNEWAFK SWVESDEYTG
110 120 130 140 150
PDMIDFGLRS QQDEEFKVQY DEQMELFKKN VLEDDEFSNK YGYLGDVYGK
160 170 180 190 200
QWRAWKTTAG ETLDQLKDVI EMIKKTPDSR RLIVSAWNPE DVPSMALPPC
210 220 230 240 250
HTLFQFYVAD GKLSCQLYQR SGDIFLGIPF NIASYSLLTH LIAHECGLEV
260 270 280 290 300
GEFVHTIGDA HIYTNHFEQV EKQLAREPRP FPKLTLNPDV KSVFDFEMED
310
LTIEGYDPHP AIKAPVAV
Length:318
Mass (Da):36,893
Last modified:June 1, 2003 - v1
Checksum:i6E1FD2DCD1811F18
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP26113.1.
AE017334 Genomic DNA. Translation: AAT31356.1.
AE017225 Genomic DNA. Translation: AAT54396.1.
RefSeqiNP_844627.1. NC_003997.3.
WP_000679592.1. NZ_LHUO01000023.1.
YP_028345.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP26113; AAP26113; BA_2236.
AAT31356; AAT31356; GBAA_2236.
AAT54396; AAT54396; BAS2082.
GeneIDi1085458.
2850789.
KEGGiban:BA_2236.
bar:GBAA_2236.
bat:BAS2082.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP26113.1.
AE017334 Genomic DNA. Translation: AAT31356.1.
AE017225 Genomic DNA. Translation: AAT54396.1.
RefSeqiNP_844627.1. NC_003997.3.
WP_000679592.1. NZ_LHUO01000023.1.
YP_028345.1. NC_005945.1.

3D structure databases

ProteinModelPortaliQ81R23.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198094.BA_2236.

Protocols and materials databases

DNASUi1085458.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP26113; AAP26113; BA_2236.
AAT31356; AAT31356; GBAA_2236.
AAT54396; AAT54396; BAS2082.
GeneIDi1085458.
2850789.
KEGGiban:BA_2236.
bar:GBAA_2236.
bat:BAS2082.

Phylogenomic databases

eggNOGiENOG4105C0V. Bacteria.
COG0207. LUCA.
HOGENOMiHOG000257899.
KOiK00560.
OMAiIVYELLW.

Enzyme and pathway databases

UniPathwayiUPA00575.
BioCyciANTHRA:THYA-MONOMER.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 2 hits.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYSY_BACAN
AccessioniPrimary (citable) accession number: Q81R23
Secondary accession number(s): Q6HZ93, Q6KT87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: June 1, 2003
Last modified: November 30, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.