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Protein

Thymidylate synthase

Gene

thyA

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei25dUMPUniRule annotation1
Active sitei200NucleophileUniRule annotation1
Binding sitei2235,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei231dUMPUniRule annotation1
Binding sitei3175,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi180 – 181dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi220 – 223dUMPUniRule annotation4
Nucleotide bindingi261 – 263dUMPUniRule annotation3

GO - Molecular functioni

  • thymidylate synthase activity Source: TIGR

GO - Biological processi

  • 2'-deoxyribonucleotide biosynthetic process Source: TIGR
  • dTMP biosynthetic process Source: InterPro
  • dTTP biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:BA_2236, GBAA_2236, BAS2082
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome
  • UP000000427 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001409161 – 318Thymidylate synthaseAdd BLAST318

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi260799.BAS2082

Structurei

3D structure databases

ProteinModelPortaliQ81R23
SMRiQ81R23
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V Bacteria
COG0207 LUCA
HOGENOMiHOG000257899
KOiK00560
OMAiKQYLDLC

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q81R23-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHAENEYLN LCRHVMEHGT KKEDRTGTGT VSVFGYQMRF DLSKGFPLLT
60 70 80 90 100
TKRVPFRLVA SELLWFMKGD TNIRYLLQHN NNIWNEWAFK SWVESDEYTG
110 120 130 140 150
PDMIDFGLRS QQDEEFKVQY DEQMELFKKN VLEDDEFSNK YGYLGDVYGK
160 170 180 190 200
QWRAWKTTAG ETLDQLKDVI EMIKKTPDSR RLIVSAWNPE DVPSMALPPC
210 220 230 240 250
HTLFQFYVAD GKLSCQLYQR SGDIFLGIPF NIASYSLLTH LIAHECGLEV
260 270 280 290 300
GEFVHTIGDA HIYTNHFEQV EKQLAREPRP FPKLTLNPDV KSVFDFEMED
310
LTIEGYDPHP AIKAPVAV
Length:318
Mass (Da):36,893
Last modified:June 1, 2003 - v1
Checksum:i6E1FD2DCD1811F18
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA Translation: AAP26113.1
AE017334 Genomic DNA Translation: AAT31356.1
AE017225 Genomic DNA Translation: AAT54396.1
RefSeqiNP_844627.1, NC_003997.3
WP_000679592.1, NZ_PPES01000017.1
YP_028345.1, NC_005945.1

Genome annotation databases

EnsemblBacteriaiAAP26113; AAP26113; BA_2236
AAT31356; AAT31356; GBAA_2236
AAT54396; AAT54396; BAS2082
GeneIDi1085458
2850789
KEGGiban:BA_2236
bar:GBAA_2236
bat:BAS2082
PATRICifig|198094.11.peg.2206

Similar proteinsi

Entry informationi

Entry nameiTYSY_BACAN
AccessioniPrimary (citable) accession number: Q81R23
Secondary accession number(s): Q6HZ93, Q6KT87
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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